Journal : Acta Chromatographica
Article : A simple HPLC method for determining 2-(3-chlorophenyloamino)-5-(2,4-dihydroxyphenyl)-1,3,4-thiadiazole in brain and plasma of animals: Application to a pharmacokinetic study

Authors :
Mansoor, S.
Georgia Institute of Technology School of Chemical and Biomolecular Engineering Atlanta GA 30332-0100 USA,
Tas, C.
Georgia Institute of Technology School of Chemical and Biomolecular Engineering Atlanta GA 30332-0100 USA, ctas@gata.edu.tr,
Patel, D. S.
Pramukh Swami Science and H.D. Patel Arts College Chemistry Department Sarva Vidyalaya Campus Kadi 382 715 Gujarat India,
Sharma, N.
BA Research India Ltd. Bio-Analytical Laboratory Bodakdev, Ahmedabad 380054 Gujarat India,
Patel, M. C.
Pramukh Swami Science and H.D. Patel Arts College Chemistry Department Sarva Vidyalaya Campus Kadi 382 715 Gujarat India,
Patel, B. N.
BA Research India Ltd. Bio-Analytical Laboratory Bodakdev, Ahmedabad 380054 Gujarat India, bhavinpatel27@rediffmail.com,
Shrivastav, P. S.
Gujarat University Department of Chemistry, School of Sciences Navrangpura, Ahmedabad 380009 Gujarat India, pranav_shrivastav@yahoo.com,
Sanyal, M.
St. Xavier’s College Department of Chemistry Navrangpura, Ahmedabad 380009 Gujarat India,
Wang, G. Y.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P. R. China,
Shi, Y. P.
Lanzhou University of Technology School of Petroleum and Chemical Engineering Lanzhou 730050 P. R. China, shiyp@licp.cas.cn,
Akowuah, G. A.
UCSI University Faculty of Applied Sciences No. 1, Jalan Menara Gading, UCSI Heights 56000 Kuala Lumpur Malaysia, wuahmy@yahoo.com,
Okechukwu, P. N.
UCSI University Faculty of Pharmaceutical Sciences No. 1, Jalan Menara Gading, UCSI Heights 56000 Kuala Lumpur Malaysia,
Chiam, N. C.
UCSI University Faculty of Pharmaceutical Sciences No. 1, Jalan Menara Gading, UCSI Heights 56000 Kuala Lumpur Malaysia,
Raszewski, G.
Institute of Agricultural Medicine Department of Pathophysiology Lublin Poland, grasz@galen.imw.lublin.pl,
Juszczak, M.
Institute of Agricultural Medicine Department of Medical Biology Lublin Poland,
Lemieszek, M. K.
Institute of Agricultural Medicine Department of Medical Biology Lublin Poland,
Matysiak, J.
University of Life Sciences Department of Chemistry Lublin Poland,
Niewiadomy, A.
University of Life Sciences Department of Chemistry Lublin Poland,
Rzeski, W.
Maria Curie-Sklodowska University Department of Virology and Immunology, Institute of Microbiology and Biotechnology Lublin Poland,
Abstract : The development, optimization, and validation of a new high-performance liquid chromatography ultraviolet (HPLC-UV) method is presented for determining 2-(3-chlorophenyloamino)-5-(2,4-dihydroxy-phenyl)-1,3,4-thiadiazole (ClABT) in biological samples of rat plasma and brain tissue. ClABT was extracted directly from a plasma supernatant fraction following protein precipitation with acetonitrile and high speed centrifugation. Reverse phase HPLC separation was performed using an ODS-2 Hypersil column with the mobile phase consisting of 0.05 M triethylammonium phosphate buffer solution in acetonitrile and methanol (120:280:600, v/v/v), at room temperature, 1.2 mL min-1flow rate, and UV-diode-array detection (DAD), at 335 nm. A linear response was obtained between 12.5 and 2000 ng mL-1at analytically acceptable levels of precision (intra/inter-day) and accuracy. Mean recoveries ranged from 92.7% to 107.9%. It was concluded that the method was specific and precise and thus suitable for quantitative analysis in clinical pharmacokinetic studies of ClABT.

Keywords : 2-(3-chlorophenyloamino)-5-(2 4-dihydroxy-phenyl)-1,3, 4-thiadiazole (ClABT), HPLC-UV, pharmacokinetic study,
Publishing house : University of Silesia in Katowice
Publication date : 2014
Number : Vol. 26, no. 2
Page : 255 – 266

Bibliography
: 1 N. Siddiqui, P. Ahujaa, W. Ahsana, S.N. Pandeyab, and M. Shamsher, J. Chem. Pharm. Res., 1, 19 (2009)
2 F. Vergne, P. Bernardelli, E. Lorthiois, N. Pham, E Proust, Ch. Oliveira, A. Mafroud, F. Roye, R. Wrigglesworth, J.K. Schellhaas, M.R. Barvian, F. Moreau, M. Idrissi, A. Tertre, B. Bertin, M. Coupe, P. Berna, and P. Soulard, Bioorg. Med. Chem. Lett., 14, 4607 (2004)
3 K.Y. Jung, S.K. Kim, Z.G. Gao, A.S. Gross, N. Melman, K.A. Jacobson, and Y. Ch. Kim, Bioorg. Med. Chem., 12, 613 (2004)
4 P. Bhattacharya, J. Leonard, and K. Roy, Bioorg. Med. Chem., 13, 1159 (2005)
5 J. Matysiak and A. Niewiadomy, Synth. Commun. Chem., 36, 1621 (2006)
6 W. Rzeski, J. Matysiak, and M. Kandefer-Szerszeń, Bioorg. Med. Chem., 15, 3201 (2007)
7 M. Juszczak, J. Matysiak, W. Brzana, A. Niewiadomy, and W. Rzeski, Arzneim. Forsch. Drug Res., 58, 353 (2008)
8 M.S. Chernov’yants, I.V. Burykin, and N.V. Aleshina, J. Anal. Chem., 66, 280 (2011)
9 J.A. Stewart, C.C. Ackerly, R.A. Newman, and I.H. Krakoff, Cancer Chemother. Pharmacol., 16, 287 (1986)
10 E. Tomori, E. Tormasi, M. Varga, and J. Borlak, J. Chromatogr. B Biomed. Sci. Appl., 705, 105 (1998)
11 J.F. Borzelleca, J.L. Egle Jr., G.R. Hennigar, H.H. Klein, E.J. Kuchar, R.W. Lane, and P.S. Larson, Toxicol. Appl. Pharmacol., 56, 164 (1980)
12 H. Wong, J. Lee, J.B. Camden, and S.D. Weitman, J. Chromatogr. B., 765, 55 (2001)
13 Guidance for Industry, Bioanalytical Method Validation, U.S. Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research (2001)
14 Y. Jiang, Y. Zhang, and Z.R. Zhang, J. Chromatogr. B., 791, 285 (2003)
15 J.I.D. Wibawa, D. Fowkes, P.N. Shaw, and D.A. Barrett, J. Chromatogr. B., 774, 141 (2002)
16 C.L. Cheng, C.H. Chou, and O.Y. Hu, J. Chromatogr. B., 769, 297 (2002)
17 M. Zaffaroni, C. Mucignat, T. Pecere, G. Zagotto, R. Frapolli, M D’Incalai, and M. Zucchetti, J. Chromatogr. B., 796, 113 (2003)
18 F. Wu, Z. Zhang, and H. Ding, J. Chromatogr. Sci., 44, 12 (2006)
19 H. Wong, L. Jia, J.B. Camden, and S.D. Weitman, J. Chromatogr. B., 765, 55 (2001)
20 B. Narola, A.S. Singh, P.R. Santhakumar, and T.G. Chandrashekhar, Anal. Chem. Insights., 5, 37 (2010)
DOI :
Qute : Mansoor, S. ,Tas, C. ,Patel, D. S. ,Sharma, N. ,Patel, M. C. ,Patel, B. N. ,Shrivastav, P. S. ,Sanyal, M. ,Wang, G. Y. ,Shi, Y. P. ,Akowuah, G. A. ,Okechukwu, P. N. ,Chiam, N. C. ,Raszewski, G. ,Juszczak, M. ,Lemieszek, M. K. ,Matysiak, J. ,Niewiadomy, A. ,Rzeski, W. ,Rzeski, W. , A simple HPLC method for determining 2-(3-chlorophenyloamino)-5-(2,4-dihydroxyphenyl)-1,3,4-thiadiazole in brain and plasma of animals: Application to a pharmacokinetic study. Acta Chromatographica Vol. 26, no. 2/2014
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