A simple and sensitive RP-HPLC method for simultaneous estimation of torsemide and spironolactone in combined tablet dosage form

Czasopismo : Acta Chromatographica
Tytuł artykułu : A simple and sensitive RP-HPLC method for simultaneous estimation of torsemide and spironolactone in combined tablet dosage form

Autorzy :
Sajewicz, M.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Gontarska, M.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Kronenbach, D.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Berry, E.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Kowalska, T.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland, teresa.kowalska@us.edu.pl,
Deshpande, P. B.
A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 MH India, padmanabh77@yahoo.co.in,
Gandhi, S. V.
A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 MH India,
Gaikwad, N. V.
A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 MH India,
Khandagle, K. S.
A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 MH India,
Abstrakty : Torsemide (TSM) is a loop diuretic used in the treatment of edema, and spironolactone (SPL) is a potassium-sparing diuretic used in hypokalemia. Both are potential diuretics used in combination to treat congestive heart failure. This paper describes a simple, sensitive, accurate, and validated reverse-phase high-performance liquid chromatographic (RP-HPLC) method for the simultaneous quantification of these compounds as the bulk drug and in tablet dosage forms. Separation was carried out on Jasco HPLC system equipped with Neosphere C8 column (150 × 4.6 mm i.d.) and UV/VIS detector using acetonitrile:10 mM potassium dihydrogen phosphate buffer in ratio of 60:40 (υ/υ) as the mobile phase, and detection was carried out at 240 nm. Results were linear in the range of 2–12 μg mL-1for TSM and 5–30 μg mL-1for SPL. The method was successfully applied for the analysis of drugs in pharmaceutical formulation. Results of the analysis were validated statistically and by recovery studies.

Słowa kluczowe : RP-HPLC, torsemide, spironolactone, tablet dosage form,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2012
Numer : Vol. 24, no. 1
Strony : 15 – 22
Bibliografia : 1 http://www.wikipedia.com/torsemide (accessed on 03/02/09)
2 http://www.wikipedia.com/spironolactone (accessed on 03/02/09)
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DOI :
Cytuj : Sajewicz, M. ,Gontarska, M. ,Kronenbach, D. ,Berry, E. ,Kowalska, T. ,Deshpande, P. B. ,Gandhi, S. V. ,Gaikwad, N. V. ,Khandagle, K. S. , A simple and sensitive RP-HPLC method for simultaneous estimation of torsemide and spironolactone in combined tablet dosage form. Acta Chromatographica Vol. 24, no. 1/2012
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