Journal : Acta Chromatographica
Article : A validated HPLC method for estimation of metoprolol in human plasma

Authors :
Sherma, J.
Department of Chemistry, Lafayette College, Easton, PA 18042, USA,
Vrchotová, N.
Institute of Systems Biology and Ecology AS CR, Branišovská 31, 370 05 České Budějovice, Czech Republic,
Sivakumar, T.
Department of Pharmacy, Faculty of Engineering and Technology, Annamalai University, Annamalainagar, Tamil Nadu-608 002, India,
Petruczynik, A.
Department of Inorganic Chemistry, Medical University, Staszica 6, 20-081 Lublin, Poland,
Benkő, B.
PhD School of Semmelweis University, 1085 Budapest, Üllői út 26, Hungary, Division of Pharmacology and Drug Safety, Richter Gedeon Rt., Gyömrői út 21, 1475 Budapest, P.O. Box 27, Hungary, Department of Chemistry and Biochemistry, University of Pécs, Péc,
Mori, M.
Department of Chemistry, Faculty of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu, 376-8515, Japan,
Solangi, A. R.
National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan,
Idris, A. M.
Department of Chemistry, College of Science, King Faisal University, Hofuf 31982, Saudi Arabia,
Milán-Segovia, R.
Laboratorio de Farmacia, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Ave. Manuel Nava No. 6, 78290 San Luis Potosí, S.L.P., Mexico,
Qutab, S. S.
Schazoo Laboratories (Pvt) Ltd, 45 G.T. Road, Lahore-54000, Pakistan,
Agil, M.
Faculty of Pharmacy, Hamdard University New Delhi, 110 062, India,
Abstract : A high-performance reversed-phase liquid chromatographic method for quantification of metoprolol tartrate (MT) in human plasma is presen-ted. A C18 column was used with acetonitrile–water–triethylamine 18:81:1 (v/v) as mobile phase and pinacidil monohydrate as internal standard (IS). UV detection was at 275 nm and MT and the IS were detected at retention times of 1.5 and 2.6 min, respectively. The method is sensitive with a li-mit of quantification of 20 ng mL-1. The calibration plot for MT in spiked plasma was linear in the concentration range 20–200 ng mL-1. Within-batch and total accuracy of the method ranged between 99.71% and 101.61%, and within-batch and total precision, expressed as the coefficient of varia-tion, was 0.20–2.13%. Recovery of MT from spiked plasma was 97.00% and the freeze–thaw and bench-top stability of samples ranged from 77.92 to 105.62%. The method can be successfully used for analysis of MT in human plasma during pharmacokinetic studies.

Keywords :
Publishing house : University of Silesia in Katowice
Publication date : 2007
Number : No. 19
Page : 130 – 140

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Qute : Sherma, J. ,Vrchotová, N. ,Sivakumar, T. ,Petruczynik, A. ,Benkő, B. ,Mori, M. ,Solangi, A. R. ,Idris, A. M. ,Milán-Segovia, R. ,Qutab, S. S. ,Agil, M. ,Agil, M. , A validated HPLC method for estimation of metoprolol in human plasma. Acta Chromatographica No. 19/2007