A validated liquid chromatography and tandem mass spectrometric method for simultaneous quantitation of tenofovir, emtricitabine, and efavirenz in human plasma and its pharmacokinetic application

Czasopismo : Acta Chromatographica
Tytuł artykułu : A validated liquid chromatography and tandem mass spectrometric method for simultaneous quantitation of tenofovir, emtricitabine, and efavirenz in human plasma and its pharmacokinetic application

Autorzy :
Gwarda, R. Ł.
Medical University of Lublin Department of Physical Chemistry, Chair of Chemistry 4a Chodźki Street 20-093 Lublin Poland, radoslaw.gwarda@umlub.pl,,
Tomczyszyn, A.
University of Warsaw Faculty of Chemistry 1 Pasteura Street 02-093 Warsaw Poland,
Misicka, A.
University of Warsaw Faculty of Chemistry 1 Pasteura Street 02-093 Warsaw Poland,
Dzido, T. H.
Medical University of Lublin Department of Physical Chemistry, Chair of Chemistry 4a Chodźki Street 20-093 Lublin Poland,
Xue, X. D.
Yunnan Normal University Department of Chemistry Kunming 650092 P.R. China,
Zhang, M.
East China Normal University Department of Chemistry Shanghai 200241 P.R. China,
Xie, S. M.
Yunnan Normal University Department of Chemistry Kunming 650092 P.R. China,
Yuan, L. M.
Yunnan Normal University Department of Chemistry Kunming 650092 P.R. China, yuan_limingpd@yahoo.com.cn,
Matta, M. K.
Jawaharlal Nehru Technological University University College of Pharmaceutical Sciences Hyderabad 500 085 India,
Pilli, N. R.
Jawaharlal Nehru Technological University University College of Pharmaceutical Sciences Hyderabad 500 085 India,
Rao, J. V. L. N. S.
Yalamarty College of Pharmaceutical Sciences Visakhapatnam 530 052 India, jvlnsrao@rediffmail.com,
Abstrakty : A novel, rapid, and sensitive liquid chromatography-tandem mass spectrometric method was developed and validated for the simultaneous quantification of tenofovir, emtricitabine, and efavirenz in human plasma. Nevirapine was used as an internal standard. The analytes and the internal standard were extracted from human plasma sample by solid-phase extraction technique (SPE). The reconstituted samples were chromatographed on a Chromolith ROD C18 column (50 × 4.6 mm; 5 μ) by gradient elution using a mixture of ammonium acetate buffer (5 mM) and 0.1% formic acid in acetonitrile as the mobile phase at a flow rate of 1.0 mL min-1. The calibration curve obtained was linear (r2 ≥ 0.9990) over the concentration range of 2.5–650 ng mL-1for tenofovir and 10–4000 ng mL-1for emtricitabine and efavirenz. The results of the intra- and inter-day precision and accuracy studies were well within the acceptable limits. A run time of 2.5 min for each sample made it possible to analyze more than 300 plasma samples per day. The proposed method was found to be applicable to clinical studies, and the authenticity in the measurement of clinical data is demonstrated through incurred samples reanalysis (ISR).

Słowa kluczowe : tenofovir, emtricitabine, efavirenz, solid-phase extraction (SPE), LC-MS/MS,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2015
Numer : Vol. 27, no. 1
Strony : 27 – 39
Bibliografia : 1 US Department of Health and Human Services, 2008
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DOI :
Cytuj : Gwarda, R. Ł. ,Tomczyszyn, A. ,Misicka, A. ,Dzido, T. H. ,Xue, X. D. ,Zhang, M. ,Xie, S. M. ,Yuan, L. M. ,Matta, M. K. ,Pilli, N. R. ,Rao, J. V. L. N. S. , A validated liquid chromatography and tandem mass spectrometric method for simultaneous quantitation of tenofovir, emtricitabine, and efavirenz in human plasma and its pharmacokinetic application. Acta Chromatographica Vol. 27, no. 1/2015
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