An RP-HPLC method for simultaneous analysis of, and interaction studies on, enalapril maleate and H 2 -receptor antagonists

Czasopismo : Acta Chromatographica
Tytuł artykułu : An RP-HPLC method for simultaneous analysis of, and interaction studies on, enalapril maleate and H 2 -receptor antagonists

Autorzy :
Daszykowski, M.
Silesian University Department of Chemometrics, Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Sajewicz, M.
Silesian University Department of General Chemistry and Chromatography 9 Szkolna Street 40-006 Katowice Poland,
Rzepa, J.
Silesian University Department of General Chemistry and Chromatography 9 Szkolna Street 40-006 Katowice Poland,
Hajnos, M.
Medical University of Lublin Department of Pharmacognosy 1 Chodźki Street 20-093 Lublin Poland,
Staszek, D.
Silesian University Department of General Chemistry and Chromatography 9 Szkolna Street 40-006 Katowice Poland,
Wojtal, Ł.
Silesian University Department of General Chemistry and Chromatography 9 Szkolna Street 40-006 Katowice Poland,
Kowalska, T.
Silesian University Department of General Chemistry and Chromatography 9 Szkolna Street 40-006 Katowice Poland,
Waksmundzka-Hajnos, M.
Medical University of Lublin Department of Inorganic Chemistry 6 Staszica Street 20-081 Lublin Poland,
Walczak, B.
Silesian University Department of Chemometrics, Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland, beata@us.edu.pl,
Skalicka-Woźniak, K.
Medical University of Lublin Department of Pharmacognosy with Medicinal Plant Laboratory 1 Chodzki Str. 20-093 Lublin Poland,
Markowski, W.
Medical University of Lublin Department of Physical Chemistry Staszica 6 20-081 Lublin Poland,
Świeboda, R.
Medical University of Lublin Department of Inorganic and Analytical Chemistry Staszica 6 20-081 Lublin Poland,
Głowniak, K.
Medical University of Lublin Department of Pharmacognosy with Medicinal Plant Laboratory 1 Chodzki Str. 20-093 Lublin Poland,
Sultana, N.
University of Karachi Research Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, Faculty of Pharmacy Karachi 75270 Pakistan,
Arayne, M. S.
University of Karachi Department of Chemistry Karachi 75270 Pakistan,
Naveed, S.
University of Karachi Research Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, Faculty of Pharmacy Karachi 75270 Pakistan,
Shamshad, H.
University of Karachi Research Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, Faculty of Pharmacy Karachi 75270 Pakistan,
Abstrakty : A simple, sensitive, and rapid RP-HPLC method for analysis of enalapril in the presence of H 2 -receptor antagonists has been developed and validated. Enalapril maleate was separated from H 2 -receptor antagonists by use of a 250 mm × 4.6 mm, 5-µm particle, C 18 column with 86:14 ( υ / υ ) methanol-water, pH adjusted to 3.5, as mobile phase, at a flow rate of 1.5 mL min-1. UV detection was performed at 227 nm. The retention times of enalapril maleate, ranitidine, cimetidine, and famotidine were 3, 5, 7, and 7.5 min, respectively. The detection limit for enalapril was 10 ng mL-1and the calibration plot was linear in the range 2.5–50 µg mL-1. In-vitro interaction of enalapril with the commonly administered H 2 -receptor antagonists cimetidine, ranitidine, and famotidine in simulated gastric juice at different pH and 37°C was also studied by use of this method. These studies clearly indicated that most of these H 2 -receptor antagonists bind to enalapril causing drastic changes in the availability of the drug. The HPLC method is accurate, selective, sensitive, and reproducible.

Słowa kluczowe : ACE inhibitors, enalapril maleate, H 2 -receptor antagonists, RP-HPLC, simultaneous analysis, interaction studies,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2009
Numer : Vol. 21, no. 4
Strony : 547 – 558
Bibliografia : 1 G.G. Alfred, S.G. Louis, W.R. Theodore, and M. Ferid, Goodman and Gilman’s The Pharmacological Basis of Therapeutics, McGraw-Hill, 904 (1996)
2 R.G. Pendleton, P.G. Cook, A. Shepherd-Rose, and A.W. Mangel, Pharmacol. Exp. Ther. 1 , 64–69 (1985)
3 H. Tajerzadeh and M. Hamidi, J. Pharm. Biomed. Anal., 4 , 675–680 (2001)
4 K.H. Yoon, W. Kim, J. Park, and H.J. Kim, Bull. Korean Chem. Soc., 6 , 878–880 (2004)
5 V.V. Pisarev, N.E. Moskaleva, Y.B. Zverkov, L.B. Smirnova, V.G. Belolipetskaya, and Y.V. Sukhanov, Pharm. Chem. J. 2 , 104–107 (2005)
6 W.C. Randolph, V.L. Osborne, S.S. Walkenstein, A.P. Intoccia, J. Pharm. Sci., 66 , 1148–1150 (1977)
7 G.M. Kochak, A. Rakhit, T.N. Thompson, and M.E. Hurley, J. Clin. Pharmacol., 28 , 222–227 (1988)
8 N. Sultana, M.S. Arayne, and R. Quresh, Pak. J. Pharm. Sci., 20 , 132–139 (2007)
9 T. Rosenthal, D. Ezra, Drug Saf., 13 , 157–187 (1995)
10 H. Shionoiri, Clin. Pharmacokinet., 25 , 20–58 (1993)
11 ICH harmonised tripartite guideline, Q3A(R2) (2006)
12 http://www.drugs.com/mtm/diltiazem-and-enalapril.html
13 A. Mushtaq, Essent. Med. Biochem. 4 , 176 (1982)
14 J.P. Mordes and W.E.C. Wasker, Pharmacol. Rev., 29 , 273 (1978)
15 R.K. Rude and F.R. Singer, Annu. Rev. Med., 32 , 245 (1981)
16 M.M. Pechet, E. Bobadilla, E.L. Carroll, and R.H. Hess, Am. J. Med., 43 , 696 (1967)
17 A. Beddall, Practitioner, 234 , 714 (1990)
18 E. Mayer and T.M. Adieles, World Health Stat. Q., 38 , 302 (1985)
19 R.G. Alfanso, Remington’s Pharmaceutical Science, 19th edn, Mack Publishing, Easton, Pennsylvania, pp 940–970 (1995)
20 J.R. Duffield, S.B. Hall, D.R. William, and M.I. Barnett, Safer Total Parenteral Nutrition Based on Speciation Analysis. In: G.P. Ellis and G.B. West (Eds) Progress in Medical Chemistry, Vol. 28, Elsevier, Amsterdam, 1991
21 P.R. Dallman, Semin. Hematol., 19 , 19 (1982)
22 N. Sultana, S. Arayne, A. Sana, Pak. J. Pharm. Sci., 20 , 19–25 (2007)
23 M. Cassimjee, Pharmaceutical Services, The kzn pharmacy and therapeutics bulletin, 10 (2006)
DOI :
Cytuj : Daszykowski, M. ,Sajewicz, M. ,Rzepa, J. ,Hajnos, M. ,Staszek, D. ,Wojtal, Ł. ,Kowalska, T. ,Waksmundzka-Hajnos, M. ,Walczak, B. ,Skalicka-Woźniak, K. ,Markowski, W. ,Świeboda, R. ,Głowniak, K. ,Sultana, N. ,Arayne, M. S. ,Naveed, S. ,Shamshad, H. , An RP-HPLC method for simultaneous analysis of, and interaction studies on, enalapril maleate and H 2 -receptor antagonists. Acta Chromatographica Vol. 21, no. 4/2009
facebook