Journal : Acta Chromatographica
Article : Analysis of major urinary aminothiols by high-performance liquid chromatography with ultraviolet detection

Authors :
Komsta, Ł.
Medical University of Lublin Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland, lukasz.komsta@am.lublin.pl,
Zięba, A.
The Medical University of Silesia Department of Organic Chemistry ul. Jagiellońska 4 41-200 Sosnowiec Poland, zieba@edu.sum.pl,
Prus, W.
he University of Bielsko-Biała Institute of Environmental Protection and Engineering ul. Willowa 2 43-309 Bielsko-Biała Poland,
Pacenti, M.
University of Florence Occupational Health Division, Department of Public Health Viale Morgagni 48 50100 Florence Italy,
Dugheri, S.
University of Florence Occupational Health Division, Department of Public Health Viale Morgagni 48 50100 Florence Italy,
Gagliano-Candela, R.
University of Bari Forensic Toxicology Division, Department of Internal Medicine and Public Health P.zza G. Cesare 11 70124 Bari Italy,
Strisciullo, G.
University of Bari Forensic Toxicology Division, Department of Internal Medicine and Public Health P.zza G. Cesare 11 70124 Bari Italy,
Franchi, E.
University of Florence Organic Chemistry Department/ProtEra Srl (Spin Off of Magnetic Resonance Center) Via Lastruccia 13 50019 Sesto Fiorentino, Florence Italy,
Degli Esposti, F.
Chromline Srl Via Anita Garibaldi 40 59100 Prato Italy,
Perchiazzi, N.
Chromline Srl Via Anita Garibaldi 40 59100 Prato Italy,
Boccalon, P.
University of Florence Occupational Health Division, Department of Public Health Viale Morgagni 48 50100 Florence Italy,
Arcangeli, G.
University of Florence Occupational Health Division, Department of Public Health Viale Morgagni 48 50100 Florence Italy,
Cupelli, V.
University of Florence Occupational Health Division, Department of Public Health Viale Morgagni 48 50100 Florence Italy,
Szőke, É.
Semmelweis University Department of Pharmacognosy Budapest Hungary,
Petroianu, G.
Florida International University Department of Cell Biology Miami FL USA,
Tekes, K.
Semmelweis University Department of Pharmacodynamics Nagyvárad tér 4 1089 Budapest Hungary, drtekes@gmail.com,
Benkő, B.
Division of Pharmacology and Drug Safety Richter Gedeon Rt. Budapest Hungary,
Szegi, P.
Semmelweis University Department of Pharmacodynamics Nagyvárad tér 4 1089 Budapest Hungary,
Laufer, R.
Semmelweis University Department of Pharmacology and Pharmacotherapy Budapest Hungary,
Veress, G.
State Cardiology Hospital Balatonfüred Hungary,
Kuśmierek, K.
Military University of Technology Institute of Chemistry 2 Kaliskiego Str. 00-908 Warsaw Poland,
Bald, E.
University of Lodz Department of Environmental Chemistry 163 Pomorska Str. 90-236 Lodz Poland, ebald@uni.lodz.pl,
Abstract : High-performance liquid chromatography has been used for measurement of the concentrations of total cysteine and cysteinylglycine in human urine. The method involved conversion of disulfides to their reduced counterparts by use of tris(2-carboxyethyl)phosphine hydrochloride, derivatization with 1-benzyl-2-chloropyridinium bromide, and ion-pairing reversed-phase high-performance liquid chromatographic separation with ultraviolet detection at 315 nm. The linearity of the method was validated in the ranges 50–300 and 5–50 µmol L-1urine for cysteine and cysteinylglycine, respectively; regression coefficients were better than 0.999. The detection and quantitation limits were 0.2 and 0.5 µmol L-1, respectively, for both analytes. Intra-assay and inter-assay imprecision were below 6.0%, and accuracy was 98.95–100.80%. The method was applied to urine samples donated by apparently healthy volunteers.

Keywords : cysteine, cysteinylglycine, derivatization, HPLC, urine,
Publishing house : University of Silesia in Katowice
Publication date : 2009
Number : Vol. 21, no. 3
Page : 411 – 420

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DOI :
Qute : Komsta, Ł. ,Zięba, A. ,Prus, W. ,Pacenti, M. ,Dugheri, S. ,Gagliano-Candela, R. ,Strisciullo, G. ,Franchi, E. ,Degli Esposti, F. ,Perchiazzi, N. ,Boccalon, P. ,Arcangeli, G. ,Cupelli, V. ,Szőke, É. ,Petroianu, G. ,Tekes, K. ,Benkő, B. ,Szegi, P. ,Laufer, R. ,Veress, G. ,Kuśmierek, K. ,Bald, E. ,Bald, E. , Analysis of major urinary aminothiols by high-performance liquid chromatography with ultraviolet detection. Acta Chromatographica Vol. 21, no. 3/2009
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