Journal : Acta Chromatographica
Article : Application of HPLC, Gel electrophoresis and size exclusion chromatography for separation and characterization of storage proteins of soybean and peanut

Authors :
Bhushan, R.
Indian Institute of Technology Roorkee Department of Chemistry Roorkee 247667 India, rbushfcy@iitr.ernet.in,
Dixit, S.
Indian Institute of Technology Roorkee Department of Chemistry Roorkee 247667 India,
Abstract : Certain practical aspects in the separation of large biomolecules have been discussed with the chosen examples of storage proteins from two important crops, viz, soybean and peanut. Importance of seeds and storage proteins along with classification and nomenclature of plant proteins is briefly mentioned. The present paper describes an updated knowledge and status on structural features of glycinin (major storage protein of soybean) and arachin (major storage protein of peanut) with respect to their molecular species, subunit structure, and their association-dissociation behavior by using techniques of high-performance liquid chromatography (HPLC), electrophoresis and size exclusion chromatography (SEC) which supplemented each other.

Keywords : glycinin, arachin, molecular species and subunits, high-performance liquid chromatography, electrophoresis, size exclusion chromatography,
Publishing house : University of Silesia in Katowice
Publication date : 2012
Number : Vol. 24, no. 3
Page : 323 – 349

Bibliography
: 1 P.R. Shewry and R. Casey (Eds.) Seed Proteins, Kluwer Academic Publishers, London, 1999
2 T.B. Osborne and G.F. Campbell, The Vegetable Proteins, 2nd edn., New York: Longmans, Green and Co., London, 1924, p. 154
3 W.F. Lehnhardt, P.W. Gibson, and F.T. Orthoefer, US Patent 4, 470, 267 (1983)
4 K. Liu, Soybeans Chemistry, Technology and Utilization, Chapman & Hall, New York, 1997
5 W.J. Wolf, J. Agric. Food Chem., 18, 969 (1970)
6 I. Koshiyama, in: W. Gottschalk and H.P. Muller (Eds.), Proteins Biochemistry, Genetics, Nutritive Value, Martinus Nijoff/Dr W Junk Publisher, The Hague, 1983, p. 427
7 P.E. Staswick, M.A. Hermodson, and N.C. Nielsen, J. Biol. Chem., 256, 8752 (1981)
8 A.A. Badley, D. Atkinson, H. Hauser, D. Oldani, J.P. Green, and J.M. Stubbs, Biochem. Biophys. Acta, 412, 214 (1975)
9 W.J. Wolf and D.R. Briggs, Arch. Biochem. Biophys., 76, 377 (1958)
10 S. Utsumi, T. Nakamura, and T. Mori, Agric. Biol. Chem., 8, 2139 (1987)
11 C.M.M. Lakemond, H.H.J. de Jongh, M. Hessing, H. Gruppen, and A.G.J. Voragen, J. Food Agric. Chem., 48, 1985 (2000)
12 K.L. Stone and K.R. Williams, in: J.M. Walker (Ed.) The Protein Protocols Handbook, Humana Press, Totowa, 1996
13 R. Bhushan and U. Arya, Biomed. Chromatogr., 21, 1245 (2007)
14 O.H. Lowry, N.J. Rosebrough, A.L. Farr, and R.J. Randall, J. Biol. Chem., 193, 265 (1951)
15 M. Bradford, Anal. Biochem., 72, 248 (1976)
16 M.C. Garcia, M. Torre, F. Laborda, and M.L. Marina, J. Chromatogr. A, 758, 75 (1997)
17 H.P.J. Benett, C.A. Browne, and S. Solomon, Biochemistry, 20, 4530 (1981)
18 H.P.J. Benett, C.A. Browne, and S. Solomon, J. Liq. Chromatogr., 3, 1353 (1980)
19 N. Catsimpoolas, D.A. Rogers, S.J. Circle, and E.W. Meyer, Cereal Chem., 44, 631 (1967)
20 N. Catsimpoolas, FEBS Lett., 4, 259 (1969)
21 K. Kitamura and K. Shibasaki, Agric. Biol. Chem., 39, 1509 (1975)
22 M.A. Moreira, M.A. Hermodson, B.A. Larkins, and N.C. Nielsen, J. Biol. Chem., 19, 9921 (1979)
23 P.E. Staswick and N.C. Nielsen, Arch. Biochem. Biophys., 223, 1 (1982)
24 W.J. Wolf and D.R. Briggs, Arch. Biochem. Biophys., 85, 186 (1959)
25 I. Koshiyama, Int. J. Peptide Protein Res., 4, 167 (1972)
26 R. Bhushan and U. Arya, Biomed. Chromatogr., 22, 1296 (2008)
27 A. Darbre, Practical Protein Chemistry: A Handbook, John Wiley and Sons, Chichester, 1986, p. 14
28 B.D. Hames, in: B.D. Hames and D. Rickwood (Eds.) Gel Electrophoresis of Proteins, IRL Press, Oxford, 1985, p. 7
29 J.C. Arthur Jr., in: M.L. Anson, K. Bailey, and J.T. Edsall (Eds.), Advances in Protein Chemistry, Academic Press, New York, 1953, p. 393
30 A. St. Angelo and G.E. Mann, in: Peanuts: Culture and Uses, American Peanut research and Education Association Inc., 1973, p. 559
31 R. Bhushan, G.P. Reddy, and K.R.N. Reddy, Quarterly Chem. Rev., 2, 41 (1986)
32 R. Bhushan, G.P. Reddy, and K.R.N. Reddy, in: C.B. Anfinsen, J.T. Edsall, F.M. Richards, and D.S. Eisenberg (Eds.) Advances in Protein Chemistry, Academic Press, New York, Vol. 39, 1988, p. 235
33 R. Bhushan and J. Martens, in: P.R. Shewry and R. Casey (Eds.) Seed Proteins, Kluwer Academic Publishers, London, 1999, p. 355
34 C.O. Johns and D.B. Jones, J. Biol. Chem., 28, 77 (1916)
35 F.R. Eirich and E.K. Rideal, Nature, 146, 531 (1940)
36 M.P. Tombs and M. Lowe, Biochem. J., 105, 181 (1967)
37 T. Yamada, S. Aibara, and Y. Morita, Agric. Biol. Chem., 43, 2563 (1979)
38 J. Singh and J.W. Dieckert, Prep. Biochem., 3, 53 (1973)
39 P. Johnson and E.M. Shooter, Biochem. Biophys. Acta, 5, 361 (1950)
40 T. Yamada, S. Aibara, and Y. Morita, Agric. Biol. Chem., 43, 2549 (1979)
41 K. Yotsuhashi and K. Shibasaki, J. Japan Soc. Food Sci. Technol., 20, 321 (1973)
42 K.J. Shetty and M.S.N. Rao, Indian J. Biochem. Bio., 10, 149 (1973)
43 K.J. Shetty and M.S.N. Rao, Anal. Biochem., 62, 108 (1974)
44 R. Bhushan, R.N. Goyal, and A. Agarwal, Biochem. Int., 11, 477 (1985)
45 R. Bhushan, V.K. Mahesh, and A. Varma, Biochem. Mol. Biol. Int., 38, 81 (1996)
46 R. Bhushan and K.R.N. Reddy, Int. J. Peptide Protein Res., 35, 407 (1990)
47 R. Bhushan and K.R.N. Reddy, Int. J. Peptide Protein Res., 33, 313 (1989)
48 R. Bhushan and R. Agarwal, Biomed. Chromatogr., 20, 561 (2006)
49 R. Bhushan and R. Agarwal, Proc. Natl. Acad. Sci. India, 77(A) II, 71 (2007)
DOI :
Qute : Bhushan, R. ,Dixit, S. ,Dixit, S. , Application of HPLC, Gel electrophoresis and size exclusion chromatography for separation and characterization of storage proteins of soybean and peanut. Acta Chromatographica Vol. 24, no. 3/2012
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