Journal : Acta Chromatographica
Article : Application of LC and HPTLC-densitometry for the simultaneous determination of two multicomponent mixtures containing pyridoxine hydrochloride and cyanocobalamine

Authors :
Fatemi, M. H.
University of Mazandaran Laboratory of Chemometrics, Faculty of Chemistry Babolsar Iran, mhfatemi@umz.ac.ir,
Elyasi, M.
University of Mazandaran Laboratory of Chemometrics, Faculty of Chemistry Babolsar Iran,
Sowa, I.
Medical University of Lublin Department of Analytical Chemistry Chodźki 4a 20-093 Lublin Poland, i.sowa@umlub.pl,
Zajdel, D.
Medical University of Lublin Department of Analytical Chemistry Chodźki 4a 20-093 Lublin Poland,
Kocjan, R.
Medical University of Lublin Department of Analytical Chemistry Chodźki 4a 20-093 Lublin Poland,
Pizoń, M.
Medical University of Lublin Department of Analytical Chemistry Chodźki 4a 20-093 Lublin Poland,
Wójciak-Kosior, M.
Medical University of Lublin Department of Analytical Chemistry Chodźki 4a 20-093 Lublin Poland,
Hadad, G. M.
Suez Canal University Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Ismailia 41522 Egypt, ghhadad@hotmail.com,
Abdel-Salam, R. A.
Suez Canal University Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Ismailia 41522 Egypt, dr_rasalam@yahoo.com,
Abdel-Hameed, E. A.
Suez Canal University Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Ismailia 41522 Egypt,
Abstract : Two methods were presented for the simultaneous determination of two multicomponent mixtures containing pyridoxine hydrochloride (B6) and cyanocobalamine (B12) with benfotiamine (BN) [mix. 1], or thiamine nitrate (B1) and diclofenac sodium (DC) [mix. 2]. The first high-performance liquid chromatographic (HPLC) method depends on the use of a cyanopropyl column at a flow rate of 1.5 mL min-1with a mobile phase consisting of acetonitrile-25 mM ammonium acetate, pH 3.7 (10:90, v/v), for mix. 1 or acetonitrile-25 mM ammonium acetate, pH 5.0 by gradient elution, changing the proportion of the system linearly with a time schedule program, for mix. 2. Quantitation was achieved by UV detection at 220 nm. The second method was based on HPTLC separation followed by densitometric measurement of the spots at 254 nm. The separation was achieved on HPTLC silica gel F254 plates using chloroform-ethanol-water-acetic acid (5:8:2:0.5, v/v/v/v) for mix. 1 and chloroform-ethanol-water-acetic acid (1.5:8:2:0.5, v/v/v/v) for mix. 2. The proposed methods were successfully applied for the analysis of pharmaceutical formulations containing the two multicomponent combinations.

Keywords : HPLC, HPTLC-densitometry, pyridoxine hydrochloride, cyanocobalamine,
Publishing house : University of Silesia in Katowice
Publication date : 2013
Number : Vol. 25, no. 3
Page : 431 – 450

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DOI :
Qute : Fatemi, M. H. ,Elyasi, M. ,Sowa, I. ,Zajdel, D. ,Kocjan, R. ,Pizoń, M. ,Wójciak-Kosior, M. ,Hadad, G. M. ,Abdel-Salam, R. A. ,Abdel-Hameed, E. A. ,Abdel-Hameed, E. A. , Application of LC and HPTLC-densitometry for the simultaneous determination of two multicomponent mixtures containing pyridoxine hydrochloride and cyanocobalamine. Acta Chromatographica Vol. 25, no. 3/2013
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