Application of micelle-mediated extraction in preparation of body fluids for HPLC-DAD screening of acidic and neutral drugs

Czasopismo : Acta Chromatographica
Tytuł artykułu : Application of micelle-mediated extraction in preparation of body fluids for HPLC-DAD screening of acidic and neutral drugs

Autorzy :
Petruczynik, A.
Medical University of Lublin Department of Inorganic Chemistry 4a Chodźki Str. 20-093 Lublin Poland, annapetruczynik@poczta.onet.pl,
Waksmundzka-Hajnos, M.
Medical University of Lublin Department of Inorganic Chemistry 4a Chodźki Str. 20-093 Lublin Poland,
Rykowska, I.
Adam Mickiewicz University Faculty of Chemistry Umultowska 89b 61-614 Poznań Poland, obstiwo@amu.edu.pl,
Wasiak, W.
Adam Mickiewicz University Faculty of Chemistry Umultowska 89b 61-614 Poznań Poland,
Inglot, T. W.
Medical University of Lublin Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland,
Gumieniczek, A.
anna.gumieniczek@am.lublin.pl,
Komsta, Ł.
Medical University of Lublin Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland,
Związek, R.
Medical University of Lublin Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland,
Sharaf El Din, M. K.
Mansoura University Department of Analytical Chemistry, Faculty of Pharmacy Mansoura 35516 Egypt,
Ibrahim, F.
Mansoura University Department of Analytical Chemistry, Faculty of Pharmacy Mansoura 35516 Egypt,
Eid, M. I.
Mansoura University Department of Analytical Chemistry, Faculty of Pharmacy Mansoura 35516 Egypt,
Wahba, M. E. K.
Mansoura University Department of Analytical Chemistry, Faculty of Pharmacy Mansoura 35516 Egypt, marywahba2004@yahoo.com,
El-Shaboury, S. R.
Assiut University Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Assiut 71526 Egypt,
Hussein, A. S.
Assiut University Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Assiut 71526 Egypt,
Mohamed, N. A.
Assiut University Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Assiut 71526 Egypt, mohamed_mounir2000@yahoo.com,
El-Sutohy, M. M.
Al-Azhar University, Assiut Branch Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Assiut 71524 Egypt,
Madej, K.
Jagiellonian University Department of Analytical Chemistry, Faculty of Chemistry Ingardena St. 3 30-060 Krakow Poland, madejk@chemia.uj.edu.pl,
Persona, K.
Jagiellonian University Department of Analytical Chemistry, Faculty of Chemistry Ingardena St. 3 30-060 Krakow Poland,
Nizio, M.
Jagiellonian University Department of Analytical Chemistry, Faculty of Chemistry Ingardena St. 3 30-060 Krakow Poland,
Abstrakty : Experimental conditions of cloud-point extraction (CPE) for the selected five acidic and neutral medicaments (salicylic acid, opipramol, carbamazepine, lorazepam, and alprazolam) in human plasma were studied and optimized. Separation and detection of the tested drugs were performed by the high-performance liquid chromatography with diode array detection (HPLC-DAD) method in an appropriate gradient mode using a column Nucleosil C8. Under the optimized conditions, main validation parameters were determined for all the compounds. The extraction yields (%) ranged from 54.12 to 82.17 with intra- and interday repeatability (RSD, %) 5.70–9.92 and 5.79–10.19, respectively. The detection limit was 0.5 μg mL-1for all the tested drugs with exception of salicylic acid (LOD = 2.5 μg mL-1). The linearity of the proposed method was examined for the four drugs: opipramol, carbamazepine, lorazepam, and alprazolam in the concentration range of 0.5–2.0 μg mL-1(correlation coefficient r2 = 0.995–0.999) and for salicylic acid in the concentration range of 2.5–10.0 μg mL-1(correlation coefficient r2 = 0.993). The analytical parameters for the medicaments tested in whole blood were unsatisfactory, especially in terms of extraction recovery and repeatability, and application of the developed procedure for this biological matrix requires further study.

Słowa kluczowe : micelle-mediated extraction, cloud-point extraction, screening drug analysis, acidic/neutral medicaments, body fluids,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 1
Strony : 97 – 110
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DOI :
Cytuj : Petruczynik, A. ,Waksmundzka-Hajnos, M. ,Rykowska, I. ,Wasiak, W. ,Inglot, T. W. ,Gumieniczek, A. ,Komsta, Ł. ,Związek, R. ,Sharaf El Din, M. K. ,Ibrahim, F. ,Eid, M. I. ,Wahba, M. E. K. ,El-Shaboury, S. R. ,Hussein, A. S. ,Mohamed, N. A. ,El-Sutohy, M. M. ,Madej, K. ,Persona, K. ,Nizio, M. , Application of micelle-mediated extraction in preparation of body fluids for HPLC-DAD screening of acidic and neutral drugs. Acta Chromatographica Vol. 25, no. 1/2013
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