Journal : Acta Chromatographica
Article : Assessing the efficacies of phenolic compounds in pomegranate juice using thin-layer chromatography

Authors :
Fatemi, M. H.
University of Mazandaran Faculty of Chemistry Babolsar Iran,
Hadjmohammadi, M. R.
University of Mazandaran Faculty of Chemistry Babolsar Iran, hdjmr@umz.ac.ir,
Shakeri, P.
University of Mazandaran Faculty of Chemistry Babolsar Iran,
Bhushan, R.
Indian Institute of Technology Roorkee Department of Chemistry Roorkee 247667 India, rbushfcy@iitr.ernet.in,
Lal, M.
Indian Institute of Technology Roorkee Department of Chemistry Roorkee 247667 India,
Thomas, S.
Jubilant Life Sciences Ltd. Analytical Research Department R&D Centre, C-26, Sector-59 Noida Uttar Pradesh 201 301 India, saji_thomas@jubl.com,
Paul, S. K.
Jubilant Life Sciences Ltd. Analytical Research Department R&D Centre, C-26, Sector-59 Noida Uttar Pradesh 201 301 India,
Agarwal, A.
Jubilant Life Sciences Ltd. Analytical Research Department R&D Centre, C-26, Sector-59 Noida Uttar Pradesh 201 301 India,
Mathela, C. S.
Kumaun University Department of Chemistry DSB Campus Nainital Uttarakhand 263 001 India,
Elgizawy, S. M.
Assuit University Analytical Chemistry Department, Faculty of Pharmacy 71515 Assuit Egypt,
Hassan, N. Y.
Cairo University Analytical Chemistry Department, Faculty of Pharmacy Kasr-El Aini Street 11562 Cairo Egypt,
Lotfy, H. M.
Cairo University Analytical Chemistry Department, Faculty of Pharmacy Kasr-El Aini Street 11562 Cairo Egypt,
Saleh, S. S.
October University for Modern Sciences and Arts (MSA) Analytical Chemistry Department, Faculty of Pharmacy 11787 6th October City Egypt, drsarahsalah@gmail.com,
Shakya, A.
Al-Ahliyya Amman University Faculty of Pharmacy and Medical Sciences PO Box 263 Amman 19328 Jordan, ashokshakya@hotmail.com,
Arafat, T.
Petra University Faculty of Pharmacy PO Box 961343 Amman Jordan,
Hakooz, N. M.
Zarqa University Faculty of Pharmacy Zarqa 13132 Jordan,
Abuawwad, A. N.
Jordan Center for Pharmaceutical Research PO Box 950435 Amman 11495 Jordan,
Al-Hroub, H.
Jordan Center for Pharmaceutical Research PO Box 950435 Amman 11495 Jordan,
Melhim, M.
Jordan Center for Pharmaceutical Research PO Box 950435 Amman 11495 Jordan,
Haliński, Ł.
University of Gdańsk Department of Environmental Analysis, Institute for Environmental and Human Health Protection, Faculty of Chemistry Wita Stwosza 63 80-952 Gdańsk Poland, lukaszh@chem.univ.gda.pl,
Śmigiel, D.
University of Gdańsk Department of Environmental Analysis, Institute for Environmental and Human Health Protection, Faculty of Chemistry Wita Stwosza 63 80-952 Gdańsk Poland,
Czerwicka, M.
University of Gdańsk Department of Environmental Analysis, Institute for Environmental and Human Health Protection, Faculty of Chemistry Wita Stwosza 63 80-952 Gdańsk Poland,
Paszkiewicz, M.
University of Gdańsk Department of Environmental Analysis, Institute for Environmental and Human Health Protection, Faculty of Chemistry Wita Stwosza 63 80-952 Gdańsk Poland,
Kumirska, J.
University of Gdańsk Department of Environmental Analysis, Institute for Environmental and Human Health Protection, Faculty of Chemistry Wita Stwosza 63 80-952 Gdańsk Poland,
Stepnowski, P.
University of Gdańsk Department of Environmental Analysis, Institute for Environmental and Human Health Protection, Faculty of Chemistry Wita Stwosza 63 80-952 Gdańsk Poland,
Odeh, I.
Al-Quds University Chemistry and Chemical Technology Department P.O. Box 20002 Abu-Deis Palestine,
Abu-Lafi, S.
Al-Quds University Faculty of Pharmacy P.O. Box 20002 Abu-Deis Palestine, sabulafi@science.alquds.edu,
Al-Najjar, I.
Al-Quds University Chemistry and Chemical Technology Department P.O. Box 20002 Abu-Deis Palestine,
Znini, M.
University My Ismail Laboratoire des Substances Naturelles & Synthèse et Dynamique Moléculaire, Faculté des Sciences et Techniques Errachidia Morocco,
Majidi, L.
University My Ismail Laboratoire des Substances Naturelles & Synthèse et Dynamique Moléculaire, Faculté des Sciences et Techniques Errachidia Morocco, lmajidi@yahoo.fr,,
Desjobert, J. M.
Université de Corse UMR CNRS 6134, Laboratoire de Chimie des Produits Naturels, Faculté des Sciences et Techniques Corse France,
Paolini, J.
Université de Corse UMR CNRS 6134, Laboratoire de Chimie des Produits Naturels, Faculté des Sciences et Techniques Corse France,
Costa, J.
Université de Corse UMR CNRS 6134, Laboratoire de Chimie des Produits Naturels, Faculté des Sciences et Techniques Corse France,
Xie, J.
Beijing University of Chinese Medicine School of Chinese Materia Medica Beijing 100102 China,
Zhang, Y.
Tianjin University of Commerce Department of Pharmaceutical Engineering Tianjin 300134 China,
Wang, W.
Beijing University of Chinese Medicine School of Chinese Materia Medica Beijing 100102 China, wwq57@126.com,
Hou, J.
Beijing University of Chinese Medicine School of Chinese Materia Medica Beijing 100102 China,
Zhu, M.
The First Affiliated Hospital of Nanjing University of Chinese Medicine West Phoenix Street, 150-1 210036 Nanjing China,
Duan, J.
Nanjing University of Chinese Medicine Jiangsu Key Laboratory for High Technology Research of TCM Formulae Xianlin Road 138 210023 Nanjing China,
Tang, Y. P.
Nanjing University of Chinese Medicine Jiangsu Key Laboratory for High Technology Research of TCM Formulae Xianlin Road 138 210023 Nanjing China,
Guo, J. M.
Nanjing University of Chinese Medicine Jiangsu Key Laboratory for High Technology Research of TCM Formulae Xianlin Road 138 210023 Nanjing China,
Shang, E. X.
Nanjing University of Chinese Medicine Jiangsu Key Laboratory for High Technology Research of TCM Formulae Xianlin Road 138 210023 Nanjing China,
Zhu, Z. H.
Nanjing University of Chinese Medicine Jiangsu Key Laboratory for High Technology Research of TCM Formulae Xianlin Road 138 210023 Nanjing China, zhumin0902@163.com,
Wang, T.
Hebei Normal University of Science and Technology Hebei Chestnut Engineering Technology Research Center Qinhuangdao, Hebei 066600 China, trueyeoman2008@yahoo.com.cn,
Yang, Y. D.
Hebei Normal University of Science and Technology Hebei Chestnut Engineering Technology Research Center Qinhuangdao, Hebei 066600 China,
Du, B.
Hebei Normal University of Science and Technology Hebei Chestnut Engineering Technology Research Center Qinhuangdao, Hebei 066600 China, bindufood@yahoo.com.cn,
Yu, S.
Hebei Normal University of Science and Technology Hebei Chestnut Engineering Technology Research Center Qinhuangdao, Hebei 066600 China,
Hou, W. L.
Hebei Normal University of Science and Technology Hebei Chestnut Engineering Technology Research Center Qinhuangdao, Hebei 066600 China,
Bedi, R. S.
ISF College of Pharmacy Department of Pharmacognosy Moga 142001 India,
Gauttam, V. K.
ISF College of Pharmacy Department of Pharmacognosy Moga 142001 India,
Kalia, A. N.
ISF College of Pharmacy Department of Pharmacognosy Moga 142001 India, ankalia_47@rediffmail.com,
Fakhri, E.
Kingston University School of Life Sciences Kingston upon Thames London KT1 2EE UK,
Petróczi, A.
Kingston University School of Life Sciences Kingston upon Thames London KT1 2EE UK,
Naughton, D. P.
Kingston University School of Life Sciences Kingston upon Thames London KT1 2EE UK, D.Naughton@kingston.ac.uk,
Abstract : Pomegranates (Punica granatum) have been known for centuries for their healing properties. The phenolic components of pomegranate are believed to be responsible for their antioxidant activities, hence playing a major role in reducing oxidative stress-related disease, such as cardiovascular diseases, cancer, and neurodegenerative diseases. In this study, thin-layer chromatography (TLC) was used to, first, separate and identify the phenolic constituents of pomegranate juice and, second, to assess the antioxidant efficacies of the identified compounds. Different oxidant and hydroxyl radical generating systems (Fe3+, Cu2+, H2O2, Fe2+-H2O2, and Cu2+-H2O2) were used in assessing the efficacies of phenolic compounds found in pomegranate juice. A 10 × 10 cm and 20 × 20 cm sized silica gel 60 F254 TLC plates with toluene-ethyl acetate-formic acid (60:40:10 v/v/v) as a mobile phase were used for the chromatographic separation. Two compounds, ellagic acid and gallic acid, were separated and identified. When pomegranate juice was challenged with the oxidant systems, it was observed that the phenolic compounds slowly disappeared in a concentration- and time-dependent manner. From the results, it was concluded that gallic acid had a higher antioxidant efficacy than ellagic acid. TLC has been applied for the first time to outline the antioxidant profile of pomegranate juice and assess the efficacies of phenolics using different oxidant systems, including redox-active metals and H2O2.

Keywords : pomegranate juice, thin-layer chromatography, hydrogen peroxide, metal ions, antioxidants,
Publishing house : University of Silesia in Katowice
Publication date : 2014
Number : Vol. 26, no. 3
Page : 563 – 573

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DOI :
Qute : Fatemi, M. H. ,Hadjmohammadi, M. R. ,Shakeri, P. ,Bhushan, R. ,Lal, M. ,Thomas, S. ,Paul, S. K. ,Agarwal, A. ,Mathela, C. S. ,Elgizawy, S. M. ,Hassan, N. Y. ,Lotfy, H. M. ,Saleh, S. S. ,Shakya, A. ,Arafat, T. ,Hakooz, N. M. ,Abuawwad, A. N. ,Al-Hroub, H. ,Melhim, M. ,Haliński, Ł. ,Śmigiel, D. ,Czerwicka, M. ,Paszkiewicz, M. ,Kumirska, J. ,Stepnowski, P. ,Odeh, I. ,Abu-Lafi, S. ,Al-Najjar, I. ,Znini, M. ,Majidi, L. ,Desjobert, J. M. ,Paolini, J. ,Costa, J. ,Xie, J. ,Zhang, Y. ,Wang, W. ,Hou, J. ,Zhu, M. ,Duan, J. ,Tang, Y. P. ,Guo, J. M. ,Shang, E. X. ,Zhu, Z. H. ,Wang, T. ,Yang, Y. D. ,Du, B. ,Yu, S. ,Hou, W. L. ,Bedi, R. S. ,Gauttam, V. K. ,Kalia, A. N. ,Fakhri, E. ,Petróczi, A. ,Naughton, D. P. ,Naughton, D. P. , Assessing the efficacies of phenolic compounds in pomegranate juice using thin-layer chromatography. Acta Chromatographica Vol. 26, no. 3/2014
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