Journal : Acta Chromatographica
Article : Characterization and determination of trace alkaloids in seeds extracts from Peganum harmala linn. Using LC-ESI-MS and HPLC

Authors :
Huo, H. L.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Yu, S. H.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Liu, X. Z.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Meng, Y.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Ren, Y. P.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China,
Zhang, L. T.
Hebei Medical University Department of Pharmaceutical Analysis, School of Pharmacy Shijiazhuang 050017 China, zhanglantong@263.net,
Liu, L.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Zhao, T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Cheng, X. M.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China,
Wang, C. H.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wchcxm@hotmail.com,
Wang, Z. T.
Laboratory for New Resources and Quality Evaluation of Chinese Medicines The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key 1200 Cailun Road, Zhangjiang Hi-Tech Park Shanghai 201210 China, wangzt@hotmail.com,
Abstract : Harmaline and harmine accounted for more than 70% in composition in extracts of P. harmala. More attention, however, should be paid to the other alkaloids which would be favorable or unfavorable to the efficacy and safety of the products. It was necessary to determine these trace alkaloids in the extracts; thereafter, most of them have been characterized. Diglycoside vasicine, vasicine, vasicinone, harmalol, harmol, tetrahydroharmine, 8-hydroxy-harmine, ruine, harmaline, and harmine were separated and identified with reference substances and characteristic MS spectra in extracts by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) and high-performance liquid chromatography (HPLC). Three trace alkaloids, vasicine, harmalol, and harmol were determined using the developed chromatographic separation method subsequently. The average contents of vasicine, harmalol, and harmol in extracts of ten batches were 2.53 ± 0.73, 0.54 ± 0.19, and 0.077 ± 0.03%, respectively. The total content of the three alkaloids was 3.23 ± 0.90% (from 1.81 to 4.48%). For rough estimation of all the relative alkaloids except of harmaline and harmine, the average total areas of all peaks in extracts varied from 4.35 to 26.64% detected at 220, 254, 265, 280, and 380 nm, respectively. The results indicated that area normalization method was powerless for the quality evaluation for traditional herb medicine consisting of numerous compounds with highly differential features. It might be concluded that LC-MS or HPLC could be utilized as a qualitative and quantitative analytical method for quality control of the extracts from seeds of P. harmala L.

Keywords : Peganum harmala L, extracts, alkaloids, characterization, quality control, LC-ESI-MS,
Publishing house : University of Silesia in Katowice
Publication date : 2013
Number : Vol. 25, no. 2
Page : 221 – 240

Bibliography
: 1 L. EL-Bahri and R. Chemli, Vet. Hum. Toxicol., 33, 276–277 (1991)
2 M. Massoud, J. Hossein, and S. Porooz, Iran. J. Pharmacol. Ther., 1, 1–4 (2002)
3 C.H. Wang, J. Liu, L.M. Zheng, Y.M. Lin, X.G. Zou, M. Chen, and D.J. Sun, Chin. Pharm. J., 37, 211–215 (2002)
4 J.S. Glasby, Encyclopedia of the alkaloids, Plenum Press, New York, 1975, p. 658
5 O.Y. Khan, Studies in the chemical constituents of Peganum harmala, University of Karachi Research Institute of Chemistry, Doctor thesis, Pakistan, 1990, pp. 26–41
6 B. Blum, H. Weizmann, G.M. Simpson, D. Krasilowsky, I.S. Kulcsar, and H. Merskey, Psychopharmacologia, 6, 307–310 (1964)
7 N. Ginovart, J.H. Meyer, A. Boovariwala, D. Hussey, E.A. Rabiner, S. Houle, and A.A. Wilson, J. Cereb. Blood Flow Metab., 3, 330–344 (2006)
8 M. Iurlo, G. Leone, B. Schilström, L. Linnér, G. Nomikos, P. Hertel, B. Silvestrini, and H. Svensson, Psychopharmacology, 159, 98–104 (2001)
9 Y. Song, D. Kesuma, J. Wang, Y. Deng, J. Duan, J.H. Wang, and R.Z. Qi, Biochem. Biophys. Res. Commun., 317, 128–132 (2004)
10 E.E. Wright, J.L. Bird, J.M. Feldman, Res. Commun. Chem. Pathol. Pharmacol., 24, 259–272 (1979)
11 M. Canessa, E. Jaimovich, and M. de la Fuente, J. Membr. Biol., 13, 263–282 (1973)
12 Z.Y. Song, J.R. Liu, X.L. Lu, and L.J. Wang, Chin. Med. Mat., 29, 571–573 (2006)
13 J. Jiménez, L. Riverón-Negrete, F. Abdullaev, J. Espinosa-Aguirre, and R. Rodríguez-Arnaiz, Exp. Toxicol. Pathol., 60, 381–389 (2008)
14 E.N. Gaviraj, G.R. Babu, and U.D. Murthy, Indian Drugs, 35, 471–474 (1998)
15 A. Astulla, K. Zaima, Y. Matsuno, Y. Hirasawa, W. Ekasari, A. Widyawaruyanti, N.C. Zaini, and H. Morita, Nat. Med., 62, 470–472 (2008)
16 G.C. Di, F. Delmas, E. Ollivier, R. Elias, G. Balansard, and P. Timon-David, Exp. Parasitol., 106, 67–74 (2004)
17 D. Bian, G.H. Li, and H.Q. Zhang, Acta Pharmacol. Sin., 8, 477–480 (1987)
18 J. Bruinvels and T.L. Sourkes, Eur. J. Pharmacol., 4, 31–39 (1968)
19 D.H. Aarons, G.V. Rossi, and R.F. Orzechowski, J. Pharm. Sci., 66, 1244–1248 (1977)
20 X.Y. Zheng, Z.J. Zhang, G.X. Chou, T. Wu, X.M. Cheng, C.H. Wang, and Z.T. Wang, Arch. Pharm. Res., 32, 1245–1251 (2009)
21 X.M. Chen, Y.Q. Liu, H.D. Xie, C.H. Wang, and Z.T. Wang, Chin. J. Pharm., 39, 443–446 (2008)
22 O.P. Gupta, K.K. Anand, B.J. Ghatak, and C.K. Atal, Indian J. Exp. Biol., 16, 1075–1077 (1978)
23 C.H. Wang, J. Liu, and Y.M. Lin, Chin. Hosp. Pharm. J., 22, 139–141 (2002)
24 R. Zayed and M. Wink, Z. Naturforsch. C, 60 451–458 (2005)
25 R. Kikura-Hanajiri, M. Hayashi, K. Saisho, and Y. Goda, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 825, 29–37 (2005)
26 F. Bianchi, M. Careri, C. Corradini, I. Elviri, A. Mangia, and I. Zagnoni, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 825, 193–200 (2005)
27 M.T. Galceran, E. Moyano, L. Puignou, and P. Pais, J. Chromatogr. A, 730, 185–194 (1996)
28 J.A. Duan, R.H. Zhou, S.X. Zhao, M.S. Wang, and Z.T. Che, J. Chin. Pharm. Univ., 29, 21–23 (1998)
29 X.L. Tian, D.J. Sun, and N.S. Du, J. Xinjiang Med. Univ., 26, 113–116 (2003)
30 W. Schliemann, B. Schneider, V. Wray, J. Schmidt, M. Nimtz, A. Porzel, and H. Böhm, Phytochemistry, 67, 191–201 (2006)
DOI :
Qute : Huo, H. L. ,Yu, S. H. ,Liu, X. Z. ,Meng, Y. ,Ren, Y. P. ,Zhang, L. T. ,Liu, L. ,Zhao, T. ,Cheng, X. M. ,Wang, C. H. ,Wang, Z. T. ,Wang, Z. T. , Characterization and determination of trace alkaloids in seeds extracts from Peganum harmala linn. Using LC-ESI-MS and HPLC. Acta Chromatographica Vol. 25, no. 2/2013
facebook