Journal : Acta Chromatographica
Article : Chromatographic behaviour of a series of nitro and aza steroids

Authors :
Bartnik, M.
Department of Pharmacognosy with Medicinal Plant Laboratory, Medical University, 1, Chodźki Str., 20-093 Lublin, Poland,
Kowalski, R.
Subdepartment of Food Quality Assessment and Central Apparatus Laboratory, Agricultural University, 20-950 Lublin, Akademicka 13, Poland,
Polak, B.
Department of Physical Chemistry, Faculty of Pharmacy, Medical University, Lublin, Poland,
Turło, J.
Department of Drug Technology, Medical University of Warsaw, 1, Banacha Street, 02-097 Warsaw, Poland,
Szyrwińska, K.
Department of Inorganic and Analytical Chemistry, Faculty of Pharmacy, Poznań University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, Poland,
Czauderna, M.
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland,
Bączek, T.
Medical University of Gdańsk, Department of Biopharmaceutics and Pharmacodynamics, Gdańsk, Poland,
Chilmonczyk, Z.
National Medicines Institute, 30/34, Chełmska Str., 00-725 Warsaw, Poland,
Abstract : The chromatographic properties and mass spectra of several synthetic nitro and aza steroids have been examined. Reversed-phase chromatography was performed with methanol, acetonitrile, or mixtures of these with aqueous phosphate buffer as mobile phases. The relationship between calculated lipophilicity and chromatographic capacity factors of the solutes was evaluated. Mass spectra were studied using soft ionization techniques. Calculated lipophilicity was a linear function of retention when methanol and methanol–phosphate buffer were used as mobile phases but not when acetonitrile and acetonitrile–phosphate buffer were used. It did not seem possible to detect intramolecular hydrogen-bond formation by use of chromatographic retention factors. Intramolecular hydrogen-bond formation affected the mass spectra, suggesting it affected the stability of the molecular ions.

Keywords :
Publishing house : University of Silesia in Katowice
Publication date : 2007
Number : No. 18
Page : 93 – 105

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Qute : Bartnik, M. ,Kowalski, R. ,Polak, B. ,Turło, J. ,Szyrwińska, K. ,Czauderna, M. ,Bączek, T. ,Chilmonczyk, Z. ,Chilmonczyk, Z. , Chromatographic behaviour of a series of nitro and aza steroids. Acta Chromatographica No. 18/2007