Chromatographic fractionation and analysis of the main components of eggplant (Solanum melongena L.) leaf cuticular waxes

Czasopismo : Acta Chromatographica
Tytuł artykułu : Chromatographic fractionation and analysis of the main components of eggplant (Solanum melongena L.) leaf cuticular waxes

Autorzy :
Turło, J.
Medical University of Warsaw Department of Drug Technology 1 Banacha Street 02-097 Warsaw Poland Medical University of Warsaw Faculty of Pharmacy 1 Banacha Street 02-097 Warsaw Poland, jadwiga.turlo@wum.edu.p,
Komsta, Ł.
Medical University of Lublin Chair and Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland Medical University of Lublin Chair and Department of Pharmaceutical Botany, Faculty of Pharmacy Chodźki 1 20-093 Lublin Pola, lukasz.komsta@am.lublin.pl,
Vasta, J. D.
Lafayette College Department of Chemistry Easton PA 18042-1782 USA Lafayette College Department of Biology Easton PA 18042-1782 USA, shermaj@mail.lafayette.edu,
Sajewicz, M.
Silesian University Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland, kowalska@us.edu.pl,
Raju, Ch. B. V. N.
Aurobindo Pharma Limited Srikakulam Dist. Pydibhimavaram 532 409 India Andhra University Department of Inorganic and Analytical Chemistry Visakhapatnam 530 003 India, chbvn_5@yahoo.co.in,
Shende, P.
NMIMS University School of Pharmacy and Technology Management V.L. Mehta Road, Vile Parle (W) Mumbai 400056 India, shendepravin94@yahoo.co.in,
Dhaneshwar, S. R.
Bharati Vidyapeeth University, Poona College of Pharmacy Department of Pharmaceutical Chemistry Pune 411038 India, sunil.dhaneshwar@gmail.com,
Thakur, A. K.
University Institute of Chemical Technology 1A-286, Medicinal Natural Products Research Laboratory, Pharmaceutical Division Nathalal Parikh Marg, Matunga Mumbai 400 019 Maharastra India Pharmanza Herbal Pvt. Ltd Plot no 214, Near Vadadia Patiya, Borsad-T, achalthakur@gmail.com,
Niranjan, A.
National Botanical Research Institute (Council of Scientific and Industrial Research) Central Instrumentation Facility Lucknow 226 001 India, lehrinbri@yahoo.com,
Kumar, T.
Indian Statistical Institute Agricultural and Ecological Research Unit 203 B.T. Road Kolkata 700 108 India University of Calcutta Department of Chemistry, College of Science 92 APC Road Kolkata 700 009 India, tanumikumar@yahoo.co.in,
Haliński, Ł. P.
University of Gdańsk Department of Environmental Analysis, Faculty of Chemistry Sobieskiego 18 80-952 Gdańsk Poland, lukaszh@chem.univ.gda.pl,
Abstrakty : The composition of eggplant ( Solanum melongena L.) leaf cuticular waxes was investigated. Chemical composition was determined as the first step in recognizing the importance to insect pest resistance of eggplant leaf surface chemistry. Waxes were extracted by dipping leaves into dichloromethane for 40 s. Relatively large samples were then fractionated by 'flash chromatography' on silica gel. Compounds were identified on the basis of their mass spectra from GC-MS analysis and retention data from GC-FID analysis, and quantified on the basis of peak areas from GC-FID analysis. Straight-chain alkanes and methyl-branched alkanes were the most abundant wax components. Minor quantities of esters of fatty acids with triterpene and aliphatic alcohols, free triterpene and aliphatic alcohols, sterols, and free fatty acids were also present on the eggplant leaf surface. This method is suitable for analysis of complex mixtures of plant cuticular lipids. It is also applicable to separation of samples for biological tests on insects.

Słowa kluczowe : eggplant, cuticular waxes, flash chromatography,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2009
Numer : Vol. 21, no. 1
Strony : 127 – 137
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DOI :
Cytuj : Turło, J. ,Komsta, Ł. ,Vasta, J. D. ,Sajewicz, M. ,Raju, Ch. B. V. N. ,Shende, P. ,Dhaneshwar, S. R. ,Thakur, A. K. ,Niranjan, A. ,Kumar, T. ,Haliński, Ł. P. , Chromatographic fractionation and analysis of the main components of eggplant (Solanum melongena L.) leaf cuticular waxes. Acta Chromatographica Vol. 21, no. 1/2009
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