Journal : Acta Chromatographica
Article : Chromatographic identification of two biologically important triterpenoids from the chloroform extract of Rhizophora mucronata

Authors :
Sajewicz, M.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Gontarska, M.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Kronenbach, D.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Berry, E.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Kowalska, T.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland, teresa.kowalska@us.edu.pl,
Deshpande, P. B.
A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 MH India, padmanabh77@yahoo.co.in,
Gandhi, S. V.
A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 MH India,
Gaikwad, N. V.
A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 MH India,
Khandagle, K. S.
A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 MH India,
Sateesh, J. N.
PRRM College of Pharmacy Department of Chemistry Kadapa Andhra Pradesh India, nagasateesh@gmail.com,
Subba Reedy, G. V.
JNTUA College of Engineering Department of Chemistry Pulivendula, Y.S.R (Kadapa) Andhra Pradesh India,
Jayaveera, K. N.
awaharlal Nehru Technological University Anantapur Oil Technology Research Institute Anantapur Andhra Pradesh India,
Dhayalamurthi, S.
Mother Theresa Post Graduate & Research Institute of Health Sciences Department of Chemistry, College of Pharmacy Gorimedu Puducherry India,
Anandakumar, K.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India, anandkarunakaran@gmail.com,
Jothieswari, D.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India,
Subathrai, R.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India,
Santhi, D. V.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India,
Vetrichelvan, T.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India,
Shah, D. A.
Indukaka Ipcowala College of Pharmacy P.B. No. 53 New Vallabh Vidyanagar 388 121 Dist. Anand Gujarat India, dimalgroup@yahoo.com,
Bhatt, K. K.
Indukaka Ipcowala College of Pharmacy P.B. No. 53 New Vallabh Vidyanagar 388 121 Dist. Anand Gujarat India,
Baldania, S. L.
Indukaka Ipcowala College of Pharmacy P.B. No. 53 New Vallabh Vidyanagar 388 121 Dist. Anand Gujarat India,
Tian, M.
Inha University Department of Chemical Engineering 253 Yonghyun-Dong, Nam-Ku Incheon 402-751 Korea,
Li, S.
Inha University Department of Chemical Engineering 253 Yonghyun-Dong, Nam-Ku Incheon 402-751 Korea,
Row, K. H.
Inha University Department of Chemical Engineering 253 Yonghyun-Dong, Nam-Ku Incheon 402-751 Korea, rowkho@inha.ac.kr,
Azar, P. A.
Islamic Azad University, Science and Research Branch Department of Chemistry P.O. Box 14155/4933 Tehran Iran, pabroomand@yahoo.com,
Porgham-Daryasari, A.
Islamic Azad University, Science and Research Branch Department of Chemistry P.O. Box 14155/4933 Tehran Iran,
Saber-Tehrani, M.
Islamic Azad University, Science and Research Branch Department of Chemistry P.O. Box 14155/4933 Tehran Iran,
Soleimani, M.
Islamic Azad University, Lahijan Branch Department of Chemistry, Faculty of Science P.O. Box 1616 Lahijan Iran,
Patel, M. B.
Atmiya Institute of Pharmacy Department of Pharmacognosy and Phytochemistry Yogidham Gurukul, Kalawad Road Rajkot Gujarat India, mayur.pharma@gmail.com,
Mishra, S. H.
The M.S. University of Baroda Pharmacy Department, Faculty of Technology and Engineering, Kalabhavan Vadodara 390 001 Gujarat India,
Hashemi, P.
Lorestan University Department of Chemistry, Faculty of Science Khoramabad Iran, payman_hashemi@yahoo.com,
Yarahmadi, A.
Lorestan University Department of Chemistry, Faculty of Science Khoramabad Iran,
Shamizadeh, M.
Lorestan University Department of Chemistry, Faculty of Science Khoramabad Iran,
Khademi, K.
Agriculture and Natural Resource Research Center P.O. Box 348 Khoramabad Iran,
Amir, M.
amia Hamdard Tissue Culture Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy New Delhi – 62 India,
Ahmad, A.
amia Hamdard Tissue Culture Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy New Delhi – 62 India,
Siddique, N. A.
amia Hamdard Tissue Culture Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy New Delhi – 62 India,
Mujeeb, M.
amia Hamdard Tissue Culture Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy New Delhi – 62 India, mujeeb_zaidi@yahoo.com,
Ahmad, S.
amia Hamdard Tissue Culture Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy New Delhi – 62 India,
Siddique, W. A.
Jamia Hamdard Department of Biochemistry, Faculty of Science New Delhi – 62 India,
Hridya, V. K.
Manonmaniam Sundaranar University Centre for GeoTechnology Abishekapatti, Tirunelveli Tamilnadu 627 012 India, hridyavk@yahoo.com,
Godson, P. S.
Manonmaniam Sundaranar University Centre for GeoTechnology Abishekapatti, Tirunelveli Tamilnadu 627 012 India,
Chandrasekar, N.
Manonmaniam Sundaranar University Centre for GeoTechnology Abishekapatti, Tirunelveli Tamilnadu 627 012 India,
Abstract : Mangroves are the source of several bioactive secondary metabolites and have proven activity against human, animal and plant pathogens. Rhizophora mucronata is a mangrove species belonging to the family Rhizophoraceae. Betulin and lupeol are two naturally occurring pentacyclic triterpenoids having excellent biological properties. Chloroform extract of R. mucronata, collected from Pitchavaram, Muthupett and Manakudy regions of Tamilnadu, was chromatographed on silica gel 60F254 plates with nhexane and ethyl acetate (80:20) (v/v) as the mobile phase. Derivatizations were done using anisaldehyde and sulfuric acid reagents. The triterpenoids such as betulin and lupeol were identified through HPTLC method. This is the first report for the thin layer chromatographic (TLC) identification of triterpenoids such as betulin and lupeol from R. mucronata, which will be the most valuable information to identify the antimalarial and antiviral activities.

Keywords : Mangroves, Rhizophora mucronata, betulin, lupeol, HPTLC,
Publishing house : University of Silesia in Katowice
Publication date : 2012
Number : Vol. 24, no. 1
Page : 123 – 129

Bibliography
: 1 R. Govindarajan, N. Sreevidya, M. Vijayakumar, M. Thakur, V.K. Dixit, S. Mehrotra, and P. Pushpangadan, Nat. Prod. Sci., 11, 165–169 (2005)
2 M. Thakur, S. Bhargava, and V.K. Dixit, Evid. Based Complement. Alternat. Med., 4, 419–423 (2007). doi: 10.1093/ecam/nel094
3 S.K. Chauhan, B.P. Singh, and S. Agrawal, Indian J. Pharm. Sci. 64, (2002)
4 W.M. Bandaranayake, Curr. Top. Phytochem., 14, 69–78 (1995)
5 M. Premanathan, K. Kathiresan, and K. Chandra, Int. J. Pharmacogn., 33, 75–77 (1995)
6 M. Premanathan, H. Nakashima, K. Kathiresan, N. Rajendran, and N. Yamamoto, Ind. J. Med. Res., 130, 276–279 (1996)
7 M. Premanathan, K. Chandra, S.K. Bajpai, and K. Kathiresan, Bot. Mar., 35, 321–324 (1992).
8 K. Kathiresan, T.S. Thangam, and M. Premanathan, Mangrove Halophytes: A Potential Source of Medicine. In: M.A. Khan and I.A. Ungar (Eds), Biology of Salt Tolerant Plants, University of Karachi, Pakistan, 1995, pp. 361–370
9 R.H. Cichewicz and S.A. Kouzi, Med. Res. Rev., 24, 90–114 (2004)
10 S. Fulda, I. Jeremias, H.H. Steiner, T. Pietsch, and K.M. Debatin, Int. J. Cancer, 82, 435–441 (1999)
11 R.B. Agarwal and V.D. Rangari, Indian J. Pharm., 35, 384–387 (2003)
12 T.M.D.A. Alves, T.J. Nagem, L.H. DeCarvalho, A.U. Krettli, and C.L. Zani, Planta Med., 63, 554–555 (1997)
13 S. Misra, A.K. Dutta, A. Ghosh, Phytochemistry, 24(8), 1725–1727 (1985)
14 S. Baba, K. Takara, and H. Iwasaki, J. Oceanogr., 63, 601–608 (2007)
15 H. Wagner, K. Seegert, H. Sonnenbichler, M. Ilyas, and K.P. Odenthal, Planta Med., 53, 444 (1987)
16 H. Wagner and S. Bladt, Plant Drug Analysis-A Thin Layer Chromatography Atlas, 2nd edn, Springer, Berlin, 1995
17 B. Fried and J. Sherma, Handbook of Thin-Layer Chromatography, 3rd edn, Revised and Expanded, Marcel Dekker, New York, 2003
DOI :
Qute : Sajewicz, M. ,Gontarska, M. ,Kronenbach, D. ,Berry, E. ,Kowalska, T. ,Deshpande, P. B. ,Gandhi, S. V. ,Gaikwad, N. V. ,Khandagle, K. S. ,Sateesh, J. N. ,Subba Reedy, G. V. ,Jayaveera, K. N. ,Dhayalamurthi, S. ,Anandakumar, K. ,Jothieswari, D. ,Subathrai, R. ,Santhi, D. V. ,Vetrichelvan, T. ,Shah, D. A. ,Bhatt, K. K. ,Baldania, S. L. ,Tian, M. ,Li, S. ,Row, K. H. ,Azar, P. A. ,Porgham-Daryasari, A. ,Saber-Tehrani, M. ,Soleimani, M. ,Patel, M. B. ,Mishra, S. H. ,Hashemi, P. ,Yarahmadi, A. ,Shamizadeh, M. ,Khademi, K. ,Amir, M. ,Ahmad, A. ,Siddique, N. A. ,Mujeeb, M. ,Ahmad, S. ,Siddique, W. A. ,Hridya, V. K. ,Godson, P. S. ,Chandrasekar, N. ,Chandrasekar, N. , Chromatographic identification of two biologically important triterpenoids from the chloroform extract of Rhizophora mucronata. Acta Chromatographica Vol. 24, no. 1/2012
facebook