Journal : Acta Chromatographica
Article : Comparison between conventional solid phase extraction and its simplified method for HPLC determination of five flavonoids in orange, tangerine, and lime juice samples

Authors :
Komsta, Ł.
Medical University of Lublin Chair and Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland, lukasz.komsta@am.lublin.pl,
Gowin, E.
Medical University of Lublin Chair and Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland,
Djordjević Filijović, N.
Hemofarm AD Vrsac Serbia,
Pavlović, A.
Hemofarm AD Vrsac Serbia,
Nikolić, K.
University of Belgrade, Faculty of Pharmacy Department of Pharmaceutical Chemistry Vojvode Stepe 450 PO Box 146 11000 Belgrade Serbia,
Agbaba, D.
University of Belgrade, Faculty of Pharmacy Department of Pharmaceutical Chemistry Vojvode Stepe 450 PO Box 146 11000 Belgrade Serbia, danica@pharmacy.bg.ac.rs,
Sawant, S.
Sinhgad Technical Education Society’s Smt. Kashibai Navale College of Pharmacy, Kondhwa (Bk.) Pune 411 048 Maharashtra India, sawant99@rediffmail.com,
Barge, V.
PDEA’s Seth Govind Raghunath Sable College of Pharmacy Saswad, Tal-Purandar, Dist-Pune 412301 Maharashtra India,
Tiwari, R.
School of Pharmacy and Technology Department of Pharmaceutical Chemistry, SVKM’s NMIMS Management Shirpur Dist. Dhule 425405 Maharashtra India, ravisun4@rediffmail.com,
Bonde, C.
School of Pharmacy and Technology Department of Pharmaceutical Chemistry, SVKM’s NMIMS Management Shirpur Dist. Dhule 425405 Maharashtra India,
Bothara, K.
Sinhgad Institute of Pharmacy Department of Pharmaceutical Chemistry Narhe Road Pune 410041 Maharashtra India,
Nawaz, M.
United Arab Emirates University Department of Chemistry Al-Ain 17551 UAE, nawwaz@gmail.com,
Arayne, M. S.
University of Karachi Department of Chemistry Karachi 75270 Pakistan,
Sultana, N.
University of Karachi Research Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, Faculty of Pharmacy Karachi 75270 Pakistan,
Haider, A.
University of Karachi Department of Chemistry Karachi 75270 Pakistan,
Hisaindee, S.
United Arab Emirates University Department of Chemistry Al-Ain 17551 UAE,
Youssef, R. M.
University of Alexandria Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry El-Messalah, Alexandria 21521 Egypt, rmmy1973@yahoo.com,
Malesevic, M.
Medicines and Medical Devices Agency of Serbia Vojvode Stepe 458 11 152 Belgrade Serbia,
Zivanovic, L.
University of Belgrade Faculty of Pharmacy, Department of Drug Analysis Vojvode Stepe 450 11 152 Belgrade Serbia, ljzivan@pharmacy.bg.ac.rs,
Protic, A.
University of Belgrade Faculty of Pharmacy, Department of Drug Analysis Vojvode Stepe 450 11 152 Belgrade Serbia,
Radisic, M.
University of Belgrade Faculty of Technology and Metallurgy, Department of Analytical Chemistry 11000 Belgrade Serbia,
Lausevic, M.
University of Belgrade Faculty of Technology and Metallurgy, Department of Analytical Chemistry 11000 Belgrade Serbia,
Jovic, Z.
Medicines and Medical Devices Agency of Serbia Vojvode Stepe 458 11 152 Belgrade Serbia,
Jóźwiak, A.
Department of Inorganic Chemistry Medical University of Lublin 20-093 Lublin Poland,
Jóźwiak, G.
Department of Inorganic Chemistry Medical University of Lublin 20-093 Lublin Poland,
Waksmundzka-Hajnos, M.
Department of Inorganic Chemistry Medical University of Lublin 20-093 Lublin Poland, monika.hajnos@am.lublin.pl,
Djapic, N.
Mihajlo Pupin Djure Djakovica bb 23000 Zrenjanin Serbia, ninadjapic@yahoo.com,
Lv, X. H.
Beijing University of Chemical Technology State Key Laboratory of Chemical Resource Engineering Beijing 100029 China,
Kuang, P. Q.
Beijing University of Chemical Technology State Key Laboratory of Chemical Resource Engineering Beijing 100029 China,
Yuan, Q. P.
Beijing University of Chemical Technology State Key Laboratory of Chemical Resource Engineering Beijing 100029 China, yuanqp@mail.buct.edu.cn,
Liang, H.
Beijing University of Chemical Technology State Key Laboratory of Chemical Resource Engineering Beijing 100029 China,
Zheng, G. Q.
Beijing University of Chemical Technology State Key Laboratory of Chemical Resource Engineering Beijing 100029 China,
Wang, Y.
Dalian Medical University College of Pharmacy 9 Western Lvshun South Road Dalian 116044 China,
Yin, L.
Dalian Medical University College of Pharmacy 9 Western Lvshun South Road Dalian 116044 China,
Lv, G.
Central Laboratory of Dalian Medical University 9 Western Lvshun South Road Dalian 116044 China,
Xu, Y.
Dalian Medical University College of Pharmacy 9 Western Lvshun South Road Dalian 116044 China,
Xu, L.
Dalian Medical University College of Pharmacy 9 Western Lvshun South Road Dalian 116044 China,
Qi, Y.
Dalian Medical University College of Pharmacy 9 Western Lvshun South Road Dalian 116044 China,
Zheng, L.
The First Affiliated Hospital of Dalian Medical University Dalian China, Zheng_112009@126.com,
Peng, J.
Dalian Medical University College of Pharmacy 9 Western Lvshun South Road Dalian 116044 China, jinyongpeng2008@126.com,
Asghari, A.
Semnan University Department of Chemistry Semnan 35195-363 Iran,
Barfi, B.
Semnan University Department of Chemistry Semnan 35195-363 Iran, barfi.behrooz@yahoo.com,
Barfi, A.
Arak University Department of Biology Arak Iran,
Saeidi, I.
Institute of Standard and Industrial Research of Iran Mashhad Iran,
Ghollasi-Moud, F.
Institute of Standard and Industrial Research of Iran Mashhad Iran,
Peyrovi, M.
Mazandran University Department of Chemistry Babolsar Iran,
Abstract : Although conventional solid phase extraction (SPE) is an applicable routine method for extraction of different analytes from various matrices, it requires more time and volume of solvent and sample rather than other routine laboratory extraction methods. In this study, a rapid and simplified sample preparation method based on SPE was studied by eliminating some steps, such as conditioning and washing, for extraction of diosmin, eriocitrin, narirutin, naringin, and hesperidin in orange, tangerine, and lime juice samples. The separation of these flavonoids was achieved by using a C8 column with a mobile phase comprised of water-acetonitrile-acetic acid (78:21:1, v/v/v) at a flow rate of 0.85 mL min-1and UV detection at 280 nm. To examine the applicability of this method, effective parameters such as type of adsorbent, type and volume of elution solvent, ionic strength, and pH of the sample were studied and applied to the comparison between the conventional SPE and the simplified method. The best recoveries were obtained, using the proposed method, with a small volume of citrus fruit juice (0.5 mL), silica gel (0.5 g) as adsorbent, and 3 mL of methanol as elution solvent. Limits of detection, limits of quantification, intra-day and inter-day precision of the method for the analytes were 0.0244–0.0587 μg mL-1, 0.0739–0.178 μg mL-1, 2.5 3.1%, and 3.1–4.8%, respectively.

Keywords : citrus fruit juice, flavonoid, HPLC, SPE,
Publishing house : University of Silesia in Katowice
Publication date : 2014
Number : Vol. 26, no. 1
Page : 157 – 175

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DOI :
Qute : Komsta, Ł. ,Gowin, E. ,Djordjević Filijović, N. ,Pavlović, A. ,Nikolić, K. ,Agbaba, D. ,Sawant, S. ,Barge, V. ,Tiwari, R. ,Bonde, C. ,Bothara, K. ,Nawaz, M. ,Arayne, M. S. ,Sultana, N. ,Haider, A. ,Hisaindee, S. ,Youssef, R. M. ,Malesevic, M. ,Zivanovic, L. ,Protic, A. ,Radisic, M. ,Lausevic, M. ,Jovic, Z. ,Jóźwiak, A. ,Jóźwiak, G. ,Waksmundzka-Hajnos, M. ,Djapic, N. ,Lv, X. H. ,Kuang, P. Q. ,Yuan, Q. P. ,Liang, H. ,Zheng, G. Q. ,Wang, Y. ,Yin, L. ,Lv, G. ,Xu, Y. ,Xu, L. ,Qi, Y. ,Zheng, L. ,Peng, J. ,Asghari, A. ,Barfi, B. ,Barfi, A. ,Saeidi, I. ,Ghollasi-Moud, F. ,Peyrovi, M. ,Peyrovi, M. , Comparison between conventional solid phase extraction and its simplified method for HPLC determination of five flavonoids in orange, tangerine, and lime juice samples. Acta Chromatographica Vol. 26, no. 1/2014
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