Journal : Acta Chromatographica
Article : Dealing with charged-coupled device noise in thin-layer videodensitometry. Optimization of several image-denoising techniques

Authors :
Komsta, Ł.
Medical University of Lublin Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland,,
Abstract : Charged-coupled device (CCD) noise can be a serious problem during videoscanning, especially when scanning dark plates with weakly fluorescent spots. The proper denoising of videoscans inside mathematical environments is a critical part of any advanced chemometric processing. The paper reports comparison and optimization of representative videoscan denoising by different techniques. Several kind of filters (averaging, circular, Gaussian, Savitzky-Golay, median, Wiener, FIR) and wavelet shrinkage (twelve mother wavelets from the Daubechies, Symmlet, and Coiflet family, five decomposition levels, and soft/hard thresholding) were optimized against noise autocorrelation or mean-squared error to the reference image. The reference image was obtained by grabbing and averaging 256 CCD frames. The median filter is the winner of the competition; other filters except Gaussian and wavelet shrinkage at high decomposition level are also sufficient and good ways of videoscan denoising. The Gaussian filter and wavelet shrinkage at low decomposition level performed worst and could not be recommended.

Keywords : thin-layer chromatography, videoscanning, videodensitometry, denoising, image processing,
Publishing house : University of Silesia in Katowice
Publication date : 2009
Number : Vol. 21, no. 3
Page : 355 – 367

: 1 M. Prosek and I. Vovk, in: J. Sherma and B. Fried (eds) Handbook of Thin-Layer Chromatography, Dekker, New York, 1990, pp. 277–307
2 F. Soponar, A.C. Mot, and C. Sarbu, J. Chromatogr. A, 1188 , 295 (2008)
3 Y. Reibel, M. Jung, M. Bouhifd, B. Cunin, and C. Draman, EPJ Applied Physics, 21 , 75 (2003)
4 R.C. Gonzalez and R.E. Woods, Digital Image Processing, Prentice Hall, 2007, p. 150
5 S.G. Nikolov, H. Hutter, and M. Grasserbauer, Chemom. Intell. Lab. Syst., 34 , 263 (1996)
6 D. Tschumperlé, Int. J. Comp. Vision, 68 , 65 (2006)
7 G. Vivó-Truyols and P.J. Shoenmakers, Anal. Chem., 78 , 4598 (2006)
8 T. Nagata and Y. Ebizuka, Medicinal and Aromatic Plants, Springer, 2002, p. 44
9 M. Waksmundzka-Hajnos, J. Sherma, and T. Kowalska, Thin Layer Chromatography in Phytochemistry, CRC Press, Francis and Taylor, Boca Raton, USA, 2008
10 M. Daszykowski, B. Walczak, and D.L. Massart, Chemom. Intell. Lab. Syst., 65 , 97 (2003)
Qute : Komsta, Ł. ,Komsta, Ł. , Dealing with charged-coupled device noise in thin-layer videodensitometry. Optimization of several image-denoising techniques. Acta Chromatographica Vol. 21, no. 3/2009