Journal : Acta Chromatographica
Article : Determination of amoxicillin in poultry plasma by high-performance liquid chromatography after formaldehyde derivation

Authors :
Gwarda, R. Ł.
Medical University of Lublin Department of Physical Chemistry, Chair of Chemistry 4a Chodźki Street 20-093 Lublin Poland, radoslaw.gwarda@umlub.pl,,
Tomczyszyn, A.
University of Warsaw Faculty of Chemistry 1 Pasteura Street 02-093 Warsaw Poland,
Misicka, A.
University of Warsaw Faculty of Chemistry 1 Pasteura Street 02-093 Warsaw Poland,
Dzido, T. H.
Medical University of Lublin Department of Physical Chemistry, Chair of Chemistry 4a Chodźki Street 20-093 Lublin Poland,
Xue, X. D.
Yunnan Normal University Department of Chemistry Kunming 650092 P.R. China,
Zhang, M.
East China Normal University Department of Chemistry Shanghai 200241 P.R. China,
Xie, S. M.
Yunnan Normal University Department of Chemistry Kunming 650092 P.R. China,
Yuan, L. M.
Yunnan Normal University Department of Chemistry Kunming 650092 P.R. China, yuan_limingpd@yahoo.com.cn,
Matta, M. K.
Jawaharlal Nehru Technological University University College of Pharmaceutical Sciences Hyderabad 500 085 India,
Pilli, N. R.
Jawaharlal Nehru Technological University University College of Pharmaceutical Sciences Hyderabad 500 085 India,
Rao, J. V. L. N. S.
Yalamarty College of Pharmaceutical Sciences Visakhapatnam 530 052 India, jvlnsrao@rediffmail.com,
Abdelhay, M. H.
University of Alexandria Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Elmessalah, Alexandria 21521 Egypt,
Gazy, A. A.
University of Alexandria Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Elmessalah, Alexandria 21521 Egypt,
Shaalan, R. A.
rasha_shaalan@yahoo.com,
Ashour, H. K.
University of Alexandria Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Elmessalah, Alexandria 21521 Egypt,
Krasucka, D.
National Veterinary Research Institute — National Research Institute Department of Veterinary Pharmacy Al. Partyzantów 57 24-100 Pulawy Poland,
Kowalski, C. J.
University of Life Sciences in Lublin Department of Pharmacology, Faculty of Veterinary Medicine Akademicka 12 20-033 Lublin Poland,
Osypiuk, M.
University of Life Sciences in Lublin Department of Pharmacology, Faculty of Veterinary Medicine Akademicka 12 20-033 Lublin Poland, monika.osypiuk@up.lublin.pl,
Opielak, G.
Medical University School of Lublin Human Anatomy Department Jaczewskiego 4 20-090 Lublin Poland,
Abstract : A high-performance liquid chromatographic (HPLC) method with fluorescence detection after precolumn formaldehyde derivation was developed to detect concentrations of amoxicillin (AMX) in poultry plasma. Proteins in plasma samples spiked with AMX were precipitated with a phosphate buffer and trichloroacetic acid. After precolumn treatment of the extraction product of AMX with formaldehyde under acidic and heating conditions, HPLC analysis with fluorescence (FL) detection at an excitation wavelength of 355 nm and an emission wavelength of 450 nm was performed. A mobile phase comprising acetonitrile and a buffer solution (0.05 M KH2PO4 pH = 5.6), which yielded AMX retention time 8.58 min, was suitable for detection of AMX. The calculated standard curve of the reaction product was linear, and the correlation coefficient was greater than 0.999. The limit of detection and quantification, the accuracy, and the precision were evaluated. Recoveries of spiked amoxicillin were >92%, with a coefficient of variation in the range of 0.35–0.89%. This method has been successfully applied to a pharmacokinetic study after oral administration of amoxicillin to poultry.

Keywords : amoxicillin, HPLC, validation, poultry,
Publishing house : University of Silesia in Katowice
Publication date : 2015
Number : Vol. 27, no. 1
Page : 55 – 65

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DOI :
Qute : Gwarda, R. Ł. ,Tomczyszyn, A. ,Misicka, A. ,Dzido, T. H. ,Xue, X. D. ,Zhang, M. ,Xie, S. M. ,Yuan, L. M. ,Matta, M. K. ,Pilli, N. R. ,Rao, J. V. L. N. S. ,Abdelhay, M. H. ,Gazy, A. A. ,Shaalan, R. A. ,Ashour, H. K. ,Krasucka, D. ,Kowalski, C. J. ,Osypiuk, M. ,Opielak, G. ,Opielak, G. , Determination of amoxicillin in poultry plasma by high-performance liquid chromatography after formaldehyde derivation. Acta Chromatographica Vol. 27, no. 1/2015
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