Journal : Acta Chromatographica
Article : Determination of formaldehyde in aquatic products by micellar electrokinetic capillary chromatography with 2,4-dinitrophenylhydrazine derivatization

Authors :
Xu, L. N.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Gai, F. Y.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Mu, G. F.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Gao, Y.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Liu, C. J.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Luan, F.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Abstract : Formaldehyde in aquatic products was determined by micellar electrokinetic capillary chromatography (MEKC) after derivatization with 2,4-dinitrophenylhydrazine. Separation was carried out at 25 °C and 25 kV, using a fused silica capillary (75 µ internal diameter; 50.5 cm effective length) and an ultraviolet detector set at 360 nm. The optimal background electrolyte was 20 mM sodium tetraborate and 20 mM sodium dodecyl sulfate at pH 9.0 with 3 s hydrodynamic injection at 30 mbar. Electrophoretic analysis took approximately 6.5 min. The correlation coefficient of the calibration curve was 0.999 over the concentration range 2.0–100.0 mg L-1and the LOD and LOQ values were 0.57 and 1.89 µg mL-1, respectively. The recoveries were from 83.7% to 97.2% with steam distillation as the sample pretreatment method.

Keywords : formaldehyde, capillary electrophoresis, 2,4-dinitrophenylhydrazine, aquatic products,
Publishing house : University of Silesia in Katowice
Publication date : 2012
Number : Vol. 24, no. 4
Page : 519 – 528

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DOI :
Qute : Xu, L. N. ,Gai, F. Y. ,Mu, G. F. ,Gao, Y. ,Liu, C. J. ,Luan, F. ,Luan, F. , Determination of formaldehyde in aquatic products by micellar electrokinetic capillary chromatography with 2,4-dinitrophenylhydrazine derivatization. Acta Chromatographica Vol. 24, no. 4/2012
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