Journal : Acta Chromatographica
Article : Determination of icariin in bushenqiangshen capsule in high-performance liquid chromatography with fluorescence detection by precolumn chelation with aluminum

Authors :
Sajewicz, M.
University of Silesia Department of General Chemistry and Chromatography 9 Szkolna Street 40-006 Katowice Poland,
Kowalska, T.
University of Silesia Department of General Chemistry and Chromatography 9 Szkolna Street 40-006 Katowice Poland, teresa.kowalska@us.edu.pl,
Szalka, M.
ICN Polfa Rzeszów S.A. Product Development Department 35-959 Rzeszów Poland,
Kostka, J.
Rzeszów University of Technology Department of Chemical and Process Engineering 35-959 Rzeszów,
Rokaszewski, E.
ICN Polfa Rzeszów S.A. Product Development Department 35-959 Rzeszów Poland,
Kaczmarski, K.
Rzeszów University of Technology Department of Chemical and Process Engineering 35-959 Rzeszów, kkaczmarski@prz.edu.pl,
Belal, F.
University of Mansoura Department of Analytical Chemistry, Faculty of Pharmacy 35516 Mansoura Egypt,
El-Brashy, A. M.
University of Mansoura Department of Analytical Chemistry, Faculty of Pharmacy 35516 Mansoura Egypt,
El-Enany, N.
University of Mansoura Department of Analytical Chemistry, Faculty of Pharmacy 35516 Mansoura Egypt,
Tolba, M. M.
University of Mansoura Department of Analytical Chemistry, Faculty of Pharmacy 35516 Mansoura Egypt,
Cielecka-Piontek, J.
Poznan University of Medical Sciences Department of Pharmaceutical Chemistry, Faculty of Pharmacy Grunwaldzka 6 60-780 Poznań Poland, jpiontek@ump.edu.pl,
Krause, A.
PozLab Contract Research Organization Poznań Poland,
Zalewski, P.
Poznan University of Medical Sciences Department of Pharmaceutical Chemistry, Faculty of Pharmacy Grunwaldzka 6 60-780 Poznań Poland,
Lunzer, A.
Tanaka Laboratory in Hokkaido University W 7, Kita-ku Sapporo 060-8638 Japan,
Jelińska, A.
Poznan University of Medical Sciences Department of Pharmaceutical Chemistry, Faculty of Pharmacy Grunwaldzka 6 60-780 Poznań Poland,
Sarju, N.
Universiti Teknologi Malaysia Department of Industrial Biotechnology, Faculty of Biosciences and Bioengineering 81310 Skudai, Johor Bahru, Johor Malaysia,
Abd Samad, A.
Universiti Teknologi Malaysia Department of Industrial Biotechnology, Faculty of Biosciences and Bioengineering 81310 Skudai, Johor Bahru, Johor Malaysia,
Abd Ghani, M.
Universiti Kebangsaan Malaysia School of Chemical Sciences and Food Technology 43600 UKM Bangi, Selangor Malaysia,
Ahmad, F.
Universiti Teknologi Malaysia Department of Chemistry, Faculty of Science 81310 Skudai, Johor Bahru, Johor Malaysia, farediah@kimia.fs.utm.my,
Yang, G. J.
China Pharmaceutical University School of Pharmacy Nanjing 210009 P. R. China, yanggongjun888@163.com,
Lv, L.
Yangzhou University College of Chemistry and Chemical Engineering Yangzhou 225002 P. R. China,
Abstract : A simple and sensitive method of high-performance liquid chromatography with fluorescence detection (HPLC-FLD) was developed for the determination of icariin in capsules by precolumn chelation with aluminum. In order to obtain a stable fluorescence signal, the reaction conditions of the fluorescent chelation complex between icariin and aluminum were investigated in detail. Chromatography was carried out on an Agilent Zorbax Extend C18 column (150 mm × 4.6 mm, 5.0 μm) using methanol as mobile phase at a flow rate of 1.0 mL min-1. The excitation and emission wavelengths were set at 430 and 480 nm, respectively. At optimum conditions, the calibration curve was linear in the concentration range from 0.010 to 100.0 μg mL-1with the limit of detection of 3.5 ng mL-1(S/N = 3). A comprehensive method was validated for precision and accuracy. The method described here has been successfully applied for the determination of the icariin content in a capsule with satisfactory results.

Keywords : high-performance liquid chromatography, fluorescence detection, icariin, AlCl3, capsule,
Publishing house : University of Silesia in Katowice
Publication date : 2012
Number : Vol. 24, no. 2
Page : 229 – 239

Bibliography
: 1 D.Y. Zhu, L.H. Qu, X.N. Zhang, and Y.J. Lou, Eur. J. Pharmacol., 514, 99 (2005)
2 Q.Z. Du, M. Xia, and Y. Ito, J. Chromatogr. A, 962, 239 (2002)
3 J.Q. Zhang, X.M. Wang, Z.Q. Lu, H.L. Huang, G.T. Chen, R.X. Liu, K.S. Bi, and D.A. Guo, Chromatographia, 66, 267 (2007)
4 J. Liu and Y.J. Lou, J. Pharm. Biomed. Anal., 36, 365 (2004)
5 Y.P. Zhang, W. Xu, N. Li, H.Y. Li, Z.Y. Shen, X.M. Zhang, M. Yang, W.D. Zhang, and C. Zhang, Chromatographia, 68, 245 (2008)
6 Y. Tu, L. Zhu, G.J. Wang, L.H. Zhao, and B.R. Xiang, Chromatographia, 67, 591 (2008)
7 W. Xu, Y.P. Zhang, M. Yang, Z.Y. Shen, X.M. Zhang, W.D. Zhang, and H.Y. Li, J. Pharm. Biomed. Anal., 45, 667 (2007)
8 M. Liu, H.P. Liu, X.M. Lu, C. Li, Z.L. Xiong, and F.M. Li, J. Chromatogr. B, 860, 113 (2007)
9 G.F. Deng, D.L. Wang, M.X. Meng, F. Hu, and T.W. Yao, J. Chromatogr. B, 877, 2113 (2009)
10 P.C.H. Hollman, J.M.P. Trijp, and M.N.C.P. Buysman, Anal. Chem., 68, 3511 (1996)
11 R.H. Wang and N.Q. Li, Chinese J. Anal. Chem., 22, 808 (1994)
DOI :
Qute : Sajewicz, M. ,Kowalska, T. ,Szalka, M. ,Kostka, J. ,Rokaszewski, E. ,Kaczmarski, K. ,Belal, F. ,El-Brashy, A. M. ,El-Enany, N. ,Tolba, M. M. ,Cielecka-Piontek, J. ,Krause, A. ,Zalewski, P. ,Lunzer, A. ,Jelińska, A. ,Sarju, N. ,Abd Samad, A. ,Abd Ghani, M. ,Ahmad, F. ,Yang, G. J. ,Lv, L. ,Lv, L. , Determination of icariin in bushenqiangshen capsule in high-performance liquid chromatography with fluorescence detection by precolumn chelation with aluminum. Acta Chromatographica Vol. 24, no. 2/2012
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