Journal : Acta Chromatographica
Article : Determination of morphine and codeine in serum after poppy seed consumption using gas chromatography-mass spectrometry

Authors :
Bhushan, R.
Indian Institute of Technology Roorkee Department of Chemistry Roorkee 247667 India, rbushfcy@iitr.ernet.in,
Dixit, S.
Indian Institute of Technology Roorkee Department of Chemistry Roorkee 247667 India,
Mátyus, M.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Kocsis, G.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Boldis, O.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Karvaly, G.
Scientific Institute Military Hospital-State Health Centre P.O. Box 1 H-1553 Budapest Hungary,
Magyari, E.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Fűrész, J.
Scientific Institute Military Hospital-State Health Centre P.O. Box 1 H-1553 Budapest Hungary,
Gachályi, A.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Abstract : A sensitive method was developed for the determination of free morphine and codeine in blood. This method is suitable for detection of the ingestion of illicit drugs. This test is applicable for monitoring therapeutic doses as well as stand up in the court of law. For the test we use with deuterium tagged morphine and codeine. We tag the blood specimens with deuterium prior to testing. The active ingredients (morphine and codeine) are extracted by solid phase extraction, and eventually derivatized with pentafluoro propionic anhydride (PFPA). The resulting compounds are stable and have good chromatographic characteristics. The Gas-chromatograph coupled Mass-spectrometer (GC/MS) system is used to determine the concentration of the resulting compounds using or/in the selective ion mode (SIM). The results of the target and qualifier ions were 414, 577, 361 in the case of morphine, and 282, 445 in the case of codeine. During our measurements, the graph was linear between 1 and 100 ng / mL. All the results of the qualifications were in line with the German Standards DIN 32645, which were checked with the B.E.N. program.

Keywords : metabolites, illicit drugs, morphine, free morphine, codeine, poppy seed, GC-MS, blood,
Publishing house : University of Silesia in Katowice
Publication date : 2012
Number : Vol. 24, no. 3
Page : 351 – 365

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DOI :
Qute : Bhushan, R. ,Dixit, S. ,Mátyus, M. ,Kocsis, G. ,Boldis, O. ,Karvaly, G. ,Magyari, E. ,Fűrész, J. ,Gachályi, A. ,Gachályi, A. , Determination of morphine and codeine in serum after poppy seed consumption using gas chromatography-mass spectrometry. Acta Chromatographica Vol. 24, no. 3/2012
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