Journal : Acta Chromatographica
Article : Determination of quinolonones in food of animal origin by liquid chromatography coupled with fluorescence and mass spectrometric detection

Authors :
Kuc, J.
Cracow University of Technology Department of Chemical Engineering and Technology, Department of Analytical Chemistry Warszawska 24 31-155 Cracow Poland, jkuc@chemia.pk.edu.pl,
Grochowalski, A.
Cracow University of Technology Department of Chemical Engineering and Technology, Department of Analytical Chemistry Warszawska 24 31-155 Cracow Poland,
Mach, S.
Institute of Public Health Ostrava Centre of Hygienic Laboratories Dobrá 240 739 51 Frýdek-Místek Czech Republic,
Placha, D.
VŠB-Technical University of Ostrava Centrum Nanotechnology 17 Listopadu 15/2172 708 33 Ostrava Czech Republic,
Furusawa, N.
Osaka City University Graduate School of Human Life Science Osaka 558-8585 Japan,
Stoilova, N.
Bulgarian Food Safety Agency Central Laboratory of Veterinary Control and Ecology (CLVCE) 5 “Iskarsko shousse” str., 1528 Sofia Bulgaria, nadqstoilova@abv.bg,
Surleva, A.
University of Chemical Technology and Metallurgy Analytical Chemistry Department 8 “St. Kl. Ohridski” blvd., 1756 Sofia Bulgaria,
Stoev, G.
Bulgarian Food Safety Agency Central Laboratory of Veterinary Control and Ecology (CLVCE) 5 “Iskarsko shousse” str., 1528 Sofia Bulgaria,
Abstract : High-performance liquid chromatography coupled with fluorescence (HPLC-FD) and tandem mass spectrometric detection (LC-MS/MS) was studied as a versatile tool for fast and reliable determination of nine regulated quinolones in food of animal origin (Council Regulation 2377/90/ECC). The sample pre-treatment protocol includes double step extraction with acetonitrile followed by solid phase extraction (SPE) cleanup on hydrophobic-lipophilic balance (HLB) cartridge. The separation of quinolones in HPLC-FD determination was performed on C18 Zorbax column with a gradient mixture of aqueous formic acid, methanol, and acetonitrile. A multi-wavelength excitation/emission program was used for sensitive quinolones detection. The separation efficiency of newly available chromatographic columns: Gemini C18 and Synergi Polar RP (fully porous particles), as well as Kinetex PFP and Poroshell 120 EC-C18 (core-shell particles), was studied in liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) analysis. Appropriate gradient elution program was designed for each column. Multiple reaction monitoring was used for selective determination of each quinolone. LC-MS/MS allowed quinolones determination in less than 5 min. Both methods showed detection limits below maximum residue limits for quinolones residues in food commodities.

Keywords : quinolones, food analysis, fluorescence detection, HPLC, LC-MS/MS,
Publishing house : University of Silesia in Katowice
Publication date : 2014
Number : Vol. 26, no. 4
Page : 599 – 614

Bibliography
: 1 J.A. Hernandez-Arteseros, J. Barbosa, R. Compano, and M.D. Prat, J. Chromatogr., A, 945, 1 (2002)
2 V. Andreu, C. Blasco, and Y. Pico, Trends Anal. Chem., 26, 534 (2007)
3 C.S. Chang, W.H. Wang, and C.E. Tsai, J. Food Drug Anal., 18, 87 (2010)
4 V. Jimenez, R. Companyo, and J. Guiteras, Talanta, 85, 596 (2011)
5 A.A.M. Stolker and U.A.T. Brinkman, J. Chromatogr., A, 1067, 15 (2005)
6 Commission regulation Nr 37/2010 of 22 December 2009 on Pharmacologically active substances and their classification regarding maximum residue limits in foodstuffs of animal origin, Off. J. Eur. Union, L 15/1, 20.01.2010, 2010
7 M. Seifrtova, L. Novakova, C. Lino, A. Pena, and P. Solich, Anal. Chim. Acta, 649, 158 (2009)
8 M. Hassouan, O. Ballesteros, J. Taoufiki, J. Vilchez, M. Cabrera-Aguilera, and A. Navalon, J. Chromatogr., B, 852, 625 (2007)
9 M. Lombardo-Agui, L. Gamiz-Gracia, C. Cruces-Blanco, and A.M. Garcia-Campana, J. Chromatogr., A, 1218, 4966 (2011)
10 R. Galarini, L. Fioroni, F. Angelucci, G. Tovo, and E. Cristofani, J. Chromatogr., A, 1216, 8158 (2009)
11 H. Yan, H. Wang, X. Qin, B. Liu, and J. Du, J. Pharm. Biomed. Anal., 54, 53 (2011)
12 J.H. Choi, M.I.R. Mamun, A.M. Abd El-Aty, J.H. Park, E.H. Shin, J.Y. Park, S.K. Cho, S.C. Shin, K.B. Lee, and J.H. Shim, Food Chem., 127, 1878 (2011)
13 A. Rubies, R. Vaquerizo, F. Centrich, R. Compano, M. Granados, and M.D. Prat, Talanta, 72, 269 (2007)
14 A.V. Herrera-Herrera, J. Hernandez-Borges, M.A. Rodriguez-Delgado, M. Herrero, and A. Cifuentes, J. Chromatogr., A, 1218, 7608 (2011)
15 L. Kantiani, M. Farre, and D. Barcelo, J. Chromatogr., A, 1218, 9019 (2011)
16 V. Jimenez, A. Rubies, F. Centrich, R. Companyo, and J. Guiteras, J. Chromatogr., A, 1218, 1443 (2011)
17 M.P. Hermo, E. Nemutlu, S. Kir, D. Barron, and J. Barbosa, Anal. Chim. Acta, 613, 98 (2008)
18 M.P. Hermo, D. Barron, and J. Barbosa, J. Chromatogr., A, 1201, 1 (2008)
19 O. Núnez, H. Gallart-Ayala, C.P.B. Martins, and P. Lucci, J Chromatogr., A, 1228, 298 (2012)
20 M. Farré, L. Kantiani, M. Petrovic, S. Pérez, and D. Barceló, J. Chromatogr., A, 1259, 86 (2012)
21 D. Guillarme, J. Schappler, S. Rudaz, and J.L. Veuthey, Trends Anal. Chem., 29, 15 (2010)
22 V. Jimenez, A. Rubies, F. Centrich, R. Companyo, and J. Guiteras, J. Chromatogr., A, 1218, 1443 (2011)
23 J. Shen, L. Guo, F. Xu, Q. Rao, X. Xia, X. Li, and S. Ding, Chromatographia, 71, 383 (2010)
24 H. Zhang, Y. Ren, and X. Bao, J. Pharm. Biomed. Analysis, 49, 367 (2009)
25 A. Junza, R. Amatya, D. Barrón, and J. Barbosa, J. Chromatogr., B, 879, 2601 (2011)
26 R.P. Lopes, R.C. Reyes, R. Romero-González, A. Garrido-Frenich, and J.L. Martinez-Vidal, Talanta, 89, 201 (2012); J. Chromatogr., B, 895, 39 (2012)
27 M. Lombardo-Agüí, A.M. García-Campana, L. Gámiz-Gracia, and C. Cruces-Blanco, Talanta, 93, 193 (2012)
28 M.M. Aguilera-Luiz, J.L. Martínez Vidal, R. Romero-González, and A. Garrido Frenich, Food Chem., 132, 2171 (2012)
29 M.L. Gomez-Perez, P. Plaza-Bolanos, R. Romero-Gonzalez, J.L. Martinez-Vidal, and A. Garrido-Frenich, J. Chromatogr., A, 1248, 130 (2012)
30 A. Pena, D. Chmielova, C.M. Lino, and P. Solich, J. Sep. Sci., 30, 2924 (2007)
31 A. Chiaochan, U. Koesukwiwat, S. Yudthavorasit, and N. Leepipatpiboon, Anal. Chim. Acta, 682, 117 (2010)
32 V. Jimenez, R. Companyo, and J. Guiteras, Talanta, 85, 596 (2011)
33 A. Kaufmann, P. Butcher, K. Maden, S. Walker, and M. Widmer, Anal. Chim. Acta, 700, 86 (2011)
34 N. Stoilova, A. Surleva, and G. Stoev, Food Anal. Methods, DOI 10.1007/s12161-012-9488-1 (2012)
35 Commission Decision of 12 August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results, Off. J. Eur. Communities, L221/8/17.8.2002
36 A. Kaufmann, Anal. Chim. Acta, 637, 144 (2009)
37 A. Toussaint, M. Chedin, G. Bordin, and A.R. Rodriguez, J. Chromatogr., A, 1088, 32 (2005)
38 V. Samanidou, E. Evaggelopoulou, M. Trotzmuller, X. Guo, and E. Lankmayr, J. Chromatogr., A, 1203, 115 (2008)
39 Y. Xiao, H. Chang, A. Jia, and J. Hu, J. Chromatogr., A, 1214, 100 (2008)
40 European Council Regulation on Veterinary Drug residues: Regulation (EC) 470/2009 and 37/2010
DOI :
Qute : Kuc, J. ,Grochowalski, A. ,Mach, S. ,Placha, D. ,Furusawa, N. ,Stoilova, N. ,Surleva, A. ,Stoev, G. ,Stoev, G. , Determination of quinolonones in food of animal origin by liquid chromatography coupled with fluorescence and mass spectrometric detection. Acta Chromatographica Vol. 26, no. 4/2014
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