Determination of the phospholipid and sphingolipid content in the feces of uninfected BALB/c mice and those infected with Echinostoma caproni by high performance silica gel thin layer chromatography with densitometry

Czasopismo : Acta Chromatographica
Tytuł artykułu : Determination of the phospholipid and sphingolipid content in the feces of uninfected BALB/c mice and those infected with Echinostoma caproni by high performance silica gel thin layer chromatography with densitometry

Autorzy :
Bartnik, M.
Department of Pharmacognosy with Medicinal Plant Laboratory, Medical University, 1, Chodźki Str., 20-093 Lublin, Poland,
Kowalski, R.
Subdepartment of Food Quality Assessment and Central Apparatus Laboratory, Agricultural University, 20-950 Lublin, Akademicka 13, Poland,
Polak, B.
Department of Physical Chemistry, Faculty of Pharmacy, Medical University, Lublin, Poland,
Turło, J.
Department of Drug Technology, Medical University of Warsaw, 1, Banacha Street, 02-097 Warsaw, Poland,
Szyrwińska, K.
Department of Inorganic and Analytical Chemistry, Faculty of Pharmacy, Poznań University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, Poland,
Czauderna, M.
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland,
Bączek, T.
Medical University of Gdańsk, Department of Biopharmaceutics and Pharmacodynamics, Gdańsk, Poland,
Chilmonczyk, Z.
National Medicines Institute, 30/34, Chełmska Str., 00-725 Warsaw, Poland,
Hung, Ch. Y.
Department of Biotechnology, National Formosa University, Huwei, Yunlin, 632, Taiwan,
Baboota, S.
Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi-110062, India,
Sivakumar, T.
Department of Pharmacy, Annamalai University, Annamalai Nagar, Tamil Nadu-608 002, India,
Ivanović, D.
Faculty of Pharmacy, Department of Drug Analysis, Vojvode Stepe 450, 11000 Belgrade, Serbia,
Pavlova, V.
Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Saints Cyril and Methodius University, Arhimedova 5, P.O. Box 162, 1000 Skopje, Macedonia,
Ali, J.
Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi-110062, India,
Nabi, S. A.
Analytical Research Laboratory, Department of Chemistry, Faculty of Science, Aligarh Muslim University, Aligarh-202002, India,
Murray, K. E.
Department of Chemistry, Lafayette College, Easton, PA 18042, USA,
Abstrakty : High performance thin layer chromatography (HPTLC) was used to determine the phospholipid and sphingolipid profiles in the feces of BALB/c mice infected with Echinostoma caproni (Trematoda) versus uninfected controls. Lipids extracted from fecal samples with chloroform-methanol, 2:1, were separated on silica gel HPTLC plates developed in chloroformmethanol- water, 65:25:4. Densitometric analysis of phosphatidylcholine, phosphatidylethanol-amine, and cerebroside zones was performed to compare infected versus uninfected profiles. No significant changes were found in the phospholipid or sphingolipid profiles in the feces of mice at 1 to 7 weeks post infection compared to matched uninfected controls. Although, our findings suggest that fecal polar lipids are not useful biological markers to distinguish E. caproni infections in mice from uninfected controls, the new data presented are important for future comparison in future studies involving feces from mice with heavier levels of infection and urine from infected versus uninfected mice.

Słowa kluczowe :
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2007
Numer : No. 18
Strony : 190 – 198
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DOI :
Cytuj : Bartnik, M. ,Kowalski, R. ,Polak, B. ,Turło, J. ,Szyrwińska, K. ,Czauderna, M. ,Bączek, T. ,Chilmonczyk, Z. ,Hung, Ch. Y. ,Baboota, S. ,Sivakumar, T. ,Ivanović, D. ,Pavlova, V. ,Ali, J. ,Nabi, S. A. ,Murray, K. E. , Determination of the phospholipid and sphingolipid content in the feces of uninfected BALB/c mice and those infected with Echinostoma caproni by high performance silica gel thin layer chromatography with densitometry. Acta Chromatographica No. 18/2007
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