Development and validation of HPTLC method for the estimation of diosgenin in In Vitroculture and rhizome of Dioscorea deltoidea

Czasopismo : Acta Chromatographica
Tytuł artykułu : Development and validation of HPTLC method for the estimation of diosgenin in In Vitroculture and rhizome of Dioscorea deltoidea

Autorzy :
Sajewicz, M.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Gontarska, M.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Kronenbach, D.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Berry, E.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Kowalska, T.
University of Silesia Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland, teresa.kowalska@us.edu.pl,
Deshpande, P. B.
A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 MH India, padmanabh77@yahoo.co.in,
Gandhi, S. V.
A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 MH India,
Gaikwad, N. V.
A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 MH India,
Khandagle, K. S.
A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 MH India,
Sateesh, J. N.
PRRM College of Pharmacy Department of Chemistry Kadapa Andhra Pradesh India, nagasateesh@gmail.com,
Subba Reedy, G. V.
JNTUA College of Engineering Department of Chemistry Pulivendula, Y.S.R (Kadapa) Andhra Pradesh India,
Jayaveera, K. N.
awaharlal Nehru Technological University Anantapur Oil Technology Research Institute Anantapur Andhra Pradesh India,
Dhayalamurthi, S.
Mother Theresa Post Graduate & Research Institute of Health Sciences Department of Chemistry, College of Pharmacy Gorimedu Puducherry India,
Anandakumar, K.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India, anandkarunakaran@gmail.com,
Jothieswari, D.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India,
Subathrai, R.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India,
Santhi, D. V.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India,
Vetrichelvan, T.
Adhiparasakthi College of Pharmacy Department of Pharmaceutical Analysis Melmaruvathur 603 319 Tamil Nadu India,
Shah, D. A.
Indukaka Ipcowala College of Pharmacy P.B. No. 53 New Vallabh Vidyanagar 388 121 Dist. Anand Gujarat India, dimalgroup@yahoo.com,
Bhatt, K. K.
Indukaka Ipcowala College of Pharmacy P.B. No. 53 New Vallabh Vidyanagar 388 121 Dist. Anand Gujarat India,
Baldania, S. L.
Indukaka Ipcowala College of Pharmacy P.B. No. 53 New Vallabh Vidyanagar 388 121 Dist. Anand Gujarat India,
Tian, M.
Inha University Department of Chemical Engineering 253 Yonghyun-Dong, Nam-Ku Incheon 402-751 Korea,
Li, S.
Inha University Department of Chemical Engineering 253 Yonghyun-Dong, Nam-Ku Incheon 402-751 Korea,
Row, K. H.
Inha University Department of Chemical Engineering 253 Yonghyun-Dong, Nam-Ku Incheon 402-751 Korea, rowkho@inha.ac.kr,
Azar, P. A.
Islamic Azad University, Science and Research Branch Department of Chemistry P.O. Box 14155/4933 Tehran Iran, pabroomand@yahoo.com,
Porgham-Daryasari, A.
Islamic Azad University, Science and Research Branch Department of Chemistry P.O. Box 14155/4933 Tehran Iran,
Saber-Tehrani, M.
Islamic Azad University, Science and Research Branch Department of Chemistry P.O. Box 14155/4933 Tehran Iran,
Soleimani, M.
Islamic Azad University, Lahijan Branch Department of Chemistry, Faculty of Science P.O. Box 1616 Lahijan Iran,
Patel, M. B.
Atmiya Institute of Pharmacy Department of Pharmacognosy and Phytochemistry Yogidham Gurukul, Kalawad Road Rajkot Gujarat India, mayur.pharma@gmail.com,
Mishra, S. H.
The M.S. University of Baroda Pharmacy Department, Faculty of Technology and Engineering, Kalabhavan Vadodara 390 001 Gujarat India,
Hashemi, P.
Lorestan University Department of Chemistry, Faculty of Science Khoramabad Iran, payman_hashemi@yahoo.com,
Yarahmadi, A.
Lorestan University Department of Chemistry, Faculty of Science Khoramabad Iran,
Shamizadeh, M.
Lorestan University Department of Chemistry, Faculty of Science Khoramabad Iran,
Khademi, K.
Agriculture and Natural Resource Research Center P.O. Box 348 Khoramabad Iran,
Amir, M.
amia Hamdard Tissue Culture Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy New Delhi – 62 India,
Ahmad, A.
amia Hamdard Tissue Culture Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy New Delhi – 62 India,
Siddique, N. A.
amia Hamdard Tissue Culture Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy New Delhi – 62 India,
Mujeeb, M.
amia Hamdard Tissue Culture Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy New Delhi – 62 India, mujeeb_zaidi@yahoo.com,
Ahmad, S.
amia Hamdard Tissue Culture Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy New Delhi – 62 India,
Siddique, W. A.
Jamia Hamdard Department of Biochemistry, Faculty of Science New Delhi – 62 India,
Abstrakty : A new simple, accurate, selective, precise, economical and stability-indicating high-performance thin layer chromatographic method for the analysis of diosgenin in callus and rhizome of Dioscorea deltoidea was developed and validated. The method was developed on TLC aluminium plates precoated with silica gel 60F254 using solvent system petroleum ether-isopropanol (12:1, v/v), which gives a compact spot of diosgenin (RF value 0.76 ± 0.02). Densitometric analysis of diosgenin was carried out in the absorbance mode at 366 nm after spraying with methanolic sulphuric acid. The linear regression analysis data for the calibration plots showed good linear relationship with r2 = 0.991 and 0.995 for diosgenin with respect to peak height and peak area, respectively, in the concentration range of 100–1000 ng per spot. The limits of detection and quantification for diosgenin were 16.58 and 50.25 ng per spot. The proposed method was applied for determination of diosgenin in rhizome of D. deltoidea (0.047% w/w) as well as in in vitro culture (callus) (0.092% w/w). Statistical analysis proves that the method is repeatable, selective and accurate for the estimation of diosgenin in D. deltoidea. The developed method effectively resolved the diosgenin in D. deltoidea; hence, it can be employed for routine analysis as a stability indicating method.

Słowa kluczowe : TLC, stability-indicating method, diosgenin, Dioscorea deltoidea, in vitro culture,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2012
Numer : Vol. 24, no. 1
Strony : 111 – 121
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DOI :
Cytuj : Sajewicz, M. ,Gontarska, M. ,Kronenbach, D. ,Berry, E. ,Kowalska, T. ,Deshpande, P. B. ,Gandhi, S. V. ,Gaikwad, N. V. ,Khandagle, K. S. ,Sateesh, J. N. ,Subba Reedy, G. V. ,Jayaveera, K. N. ,Dhayalamurthi, S. ,Anandakumar, K. ,Jothieswari, D. ,Subathrai, R. ,Santhi, D. V. ,Vetrichelvan, T. ,Shah, D. A. ,Bhatt, K. K. ,Baldania, S. L. ,Tian, M. ,Li, S. ,Row, K. H. ,Azar, P. A. ,Porgham-Daryasari, A. ,Saber-Tehrani, M. ,Soleimani, M. ,Patel, M. B. ,Mishra, S. H. ,Hashemi, P. ,Yarahmadi, A. ,Shamizadeh, M. ,Khademi, K. ,Amir, M. ,Ahmad, A. ,Siddique, N. A. ,Mujeeb, M. ,Ahmad, S. ,Siddique, W. A. , Development and validation of HPTLC method for the estimation of diosgenin in In Vitroculture and rhizome of Dioscorea deltoidea. Acta Chromatographica Vol. 24, no. 1/2012
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