Journal : Acta Chromatographica
Article : Development and validation of HPTLC-densitometric method for estimation of trigonelline in leaves of Abrus precatorius L. and its formulation

Authors :
Bhushan, R.
Indian Institute of Technology Roorkee Department of Chemistry Roorkee 247667 India, rbushfcy@iitr.ernet.in,
Dixit, S.
Indian Institute of Technology Roorkee Department of Chemistry Roorkee 247667 India,
Mátyus, M.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Kocsis, G.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Boldis, O.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Karvaly, G.
Scientific Institute Military Hospital-State Health Centre P.O. Box 1 H-1553 Budapest Hungary,
Magyari, E.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Fűrész, J.
Scientific Institute Military Hospital-State Health Centre P.O. Box 1 H-1553 Budapest Hungary,
Gachályi, A.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Mantovani, L.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil,
Sayago, C. T. M.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil,
Camargo, V. B.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil,
Silveira, V. F.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil,
Garcia, C. V.
Universidade Federal do Rio Grande do Sul Programa de Pós-graduação em Ciências Farmacêuticas Av. Ipiranga 2752 Lab 402 CEP 90610-000 Porto Alegre-RS Brazil,
Schapoval, E. E. S.
Universidade Federal do Rio Grande do Sul Programa de Pós-graduação em Ciências Farmacêuticas Av. Ipiranga 2752 Lab 402 CEP 90610-000 Porto Alegre-RS Brazil,
Mendez, A. S. L.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil, andreaslmendez@yahoo.com.br,
Cao, L.-W.
Jinan University Department of Chemistry Guangzhou 510632 People’s Republic of China, lwcao@126.com,
Li, C.
Jinan University Department of Chemistry Guangzhou 510632 People’s Republic of China,
Desai, D.
The Maharaja Sayajirao University of Baroda Quality Assurance Laboratory, Centre of Relevance and Excellence in Novel Drug Delivery System, Pharmacy Department, G. H. Patel Pharmacy Building, Donor’s Plaza Fatehgunj, Vadodara 390 002 Gujarat India,
Patel, G.
The Maharaja Sayajirao University of Baroda Quality Assurance Laboratory, Centre of Relevance and Excellence in Novel Drug Delivery System, Pharmacy Department, G. H. Patel Pharmacy Building, Donor’s Plaza Fatehgunj, Vadodara 390 002 Gujarat India,
Shukla, N.
The Maharaja Sayajirao University of Baroda Quality Assurance Laboratory, Centre of Relevance and Excellence in Novel Drug Delivery System, Pharmacy Department, G. H. Patel Pharmacy Building, Donor’s Plaza Fatehgunj, Vadodara 390 002 Gujarat India,
Rajput, S.
The Maharaja Sayajirao University of Baroda Quality Assurance Laboratory, Centre of Relevance and Excellence in Novel Drug Delivery System, Pharmacy Department, G. H. Patel Pharmacy Building, Donor’s Plaza Fatehgunj, Vadodara 390 002 Gujarat India, sjrajput@gmail.com,
Joshi, S. S.
National Institute of Pharmaceutical Education and Research (NIPER) Balanagar, Hyderabad 500037 India,
Nahire, R. R.
National Institute of Pharmaceutical Education and Research (NIPER) Balanagar, Hyderabad 500037 India,
Shastri, N. R.
National Institute of Pharmaceutical Education and Research (NIPER) Balanagar, Hyderabad 500037 India, svcphod@yahoo.co.in,
Surendranath, K. V.
ICICI Knowledge Park United States Pharmacopeia India Private Limited, Reference Standard Laboratory Shameerpet, Hyderabad 500078 India,
Satish, J.
ICICI Knowledge Park United States Pharmacopeia India Private Limited, Reference Standard Laboratory Shameerpet, Hyderabad 500078 India,
Kobyłka, M.
Medical University of Lublin Department of Medicinal Chemistry Jaczewskiego 4 20-090 Lublin Poland,
Komsta, Ł.
Medical University of Lublin Department of Medicinal Chemistry Jaczewskiego 4 20-090 Lublin Poland, lukasz.komsta@umlub.pl,
Adegoke, O. A.
University of Ibadan Department of Pharmaceutical Chemistry, Faculty of Pharmacy Ibadan Nigeria, ao.adegoke@mail.ui.edu.ng,
Xiang, L. L.
Chinese Academy of Sciences Laboratory of Pharmaceutical Analysis, Shanghai Institute of Materia Medica 555 Zuchongzhi Road Shanghai 201203 PR China,
Idowu, O. S.
University of Ibadan Department of Pharmaceutical Chemistry, Faculty of Pharmacy Ibadan Nigeria,
Chen, D.-Y.
Chinese Academy of Sciences Laboratory of Pharmaceutical Analysis, Shanghai Institute of Materia Medica 555 Zuchongzhi Road Shanghai 201203 PR China, dychen@mail.shcnc.ac.cn,
Omar, A. A.
Alexandria University Department of Pharmacognosy, Faculty of Pharmacy Alexandria 21521 Egypt,
Hadad, G. M.
Suez Canal University Department of Analytical Chemistry, Faculty of Pharmacy Ismailia 41522 Egypt,
Badr, J. M.
King Abdulaziz University Department of Natural Products and Alternative Medicine, Faculty of Pharmacy Jeddah 21589 Kingdom of Saudi Arabia, jihanbadr2010@hotmail.com,
Chen, Y.
Southern Medical University Key Laboratory of Chinese Drugs Pharmaceutics of Guang Dong Province, School of Traditional Chinese Medicine 510515 Guangzhou People’s Republic of China,
Li, X.
Chifeng Aike Pharmaceutical Technology Co., Ltd. 024001 Chifeng People’s Republic of China,
Chen, F.
Southern Medical University Key Laboratory of Chinese Drugs Pharmaceutics of Guang Dong Province, School of Traditional Chinese Medicine 510515 Guangzhou People’s Republic of China,
Zhu, Q.
Southern Medical University Key Laboratory of Chinese Drugs Pharmaceutics of Guang Dong Province, School of Traditional Chinese Medicine 510515 Guangzhou People’s Republic of China,
Chen, J.
Chinese Academy of Sciences Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P. R. China,
Shi, Y. P.
Chinese Academy of Sciences Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P. R. China, shiyp@licp.cas.cn,
Asmari, A.
Armed Forces Hospital Research Center Riyadh Saudi Arabia, Rkh_research@yahoo.com,
Khan, H. A.
King Saud University Prince Sultan Research Chair for Environment and Wildlife, Department of Botany and Microbiology, College of Science Riyadh Saudi Arabia,
Manthiri, R. A.
Armed Forces Hospital Research Center Riyadh Saudi Arabia,
Kumbhalkar, B. B.
Agharkar Research Institute Botany, Plant Science Division G. G. Agarkar Road Pune 411 004 India,
Rajopadhye, A. A.
Agharkar Research Institute Botany, Plant Science Division G. G. Agarkar Road Pune 411 004 India,
Upadhye, A. S.
Agharkar Research Institute Botany, Plant Science Division G. G. Agarkar Road Pune 411 004 India, upadhye.anuradha@gmail.com,
Abstract : A new, simple and reproducible HPTLC-densitometric method has been established and validated for estimation of trigonelline in leaves of Abrus precatorius L. and its herbal formulation Chatak® using ICH guidelines. Accelerated solvent extraction (ASE) as an alternative to convention techniques was also explored for the rapid extraction. The methanol extracts of leaves, its formulation, and standard solution of trigonelline were applied on silica gel F254 HPTLC plates and developed in twin chamber using mobile phase toluene-ethyl acetate-formic acid-methanol (2:3:1.8:3.8, v/v/v/v). The plates were scanned at 268 nm (λmax of trigonelline) using Camag TLC scanner 3 with CATS 4 software. A linear relationship was obtained between response (peak area) and amount of trigonelline in the range 40–200 ng spot−1; the correlation coefficient was 0.9957. ASE method has higher extraction efficiency in less time as compared to Soxhlet extraction. The HPTLC-densitometric method showed good linearity, recovery and high precision of compound. It is useful to analyze trigonelline in A. precatorius leaves and routine quality control of its marketed formulation.

Keywords : trigonelline, Abrus precatorius L, accelerated solvent extraction, HPTLC-densitometry, ICH guidelines,
Publishing house : University of Silesia in Katowice
Publication date : 2012
Number : Vol. 24, no. 3
Page : 511 – 518

Bibliography
: 1 The Wealth of India Raw Materials, Vol. 1: A, Publication and Information Directorate, Council of Scientific and Industrial Research, New Delhi, 1985, pp. 18–20
2 S. Sankaranarayanan, P. Bama, J. Ramachandran, P.T. Kalaichelvan, M. Deccaraman, M. Vijayalakshimi, R. Dhamotharan, B. Dananjeyan, and S. Sathya Bama, J. Med. Plants Res., 4, 1089–1101 (2010)
3 Y.A. Ahirrao and D.A. Patil, Indian J. Nat. Prod. Resour., 1, 85–88 (2010)
4 O.A. Georgewill and U.O. Georgewill, Eastern J. Med., 14, 23–25 (2009)
5 J. Parekh and S.V. Chanda, Turk. J. Biol., 31, 53–58 (2007)
6 C. Wambebe and S.L. Amosun, J. Ethnopharmacol., 11, 49–58 (1984)
7 A.A. Adedapo, O.A. Omoloye, and O.G. Ohore, Onderstepoort J. Vet. Res., 74, 31–36 (2007)
8 S. Ghosal and S.K. Dutta, Phytochemistry, 10, 195–198 (1971)
9 C.L. Gopu, S.S. Gilda, A.R. Paradkar, and K.R. Mahadik, Acta Chromatogr., 20(4), 709–719 (2008)
10 I.C.H. Guidelines on Analytical Method Validation, in: Proc. Int. Convention on Quality for the Pharmaceutical Industry, Toronto, 2002
DOI :
Qute : Bhushan, R. ,Dixit, S. ,Mátyus, M. ,Kocsis, G. ,Boldis, O. ,Karvaly, G. ,Magyari, E. ,Fűrész, J. ,Gachályi, A. ,Mantovani, L. ,Sayago, C. T. M. ,Camargo, V. B. ,Silveira, V. F. ,Garcia, C. V. ,Schapoval, E. E. S. ,Mendez, A. S. L. ,Cao, L.-W. ,Li, C. ,Desai, D. ,Patel, G. ,Shukla, N. ,Rajput, S. ,Joshi, S. S. ,Nahire, R. R. ,Shastri, N. R. ,Surendranath, K. V. ,Satish, J. ,Kobyłka, M. ,Komsta, Ł. ,Adegoke, O. A. ,Xiang, L. L. ,Idowu, O. S. ,Chen, D.-Y. ,Omar, A. A. ,Hadad, G. M. ,Badr, J. M. ,Chen, Y. ,Li, X. ,Chen, F. ,Zhu, Q. ,Chen, J. ,Shi, Y. P. ,Asmari, A. ,Khan, H. A. ,Manthiri, R. A. ,Kumbhalkar, B. B. ,Rajopadhye, A. A. ,Upadhye, A. S. ,Upadhye, A. S. , Development and validation of HPTLC-densitometric method for estimation of trigonelline in leaves of Abrus precatorius L. and its formulation. Acta Chromatographica Vol. 24, no. 3/2012
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