Development and validation of RP-HPLC method for simultaneous analysis of andrographolide, phyllanthin, and hypophyllanthin from herbal hepatoprotective formulation

Czasopismo : Acta Chromatographica
Tytuł artykułu : Development and validation of RP-HPLC method for simultaneous analysis of andrographolide, phyllanthin, and hypophyllanthin from herbal hepatoprotective formulation

Autorzy :
Petruczynik, A.
Medical University of Lublin Department of Inorganic Chemistry 4a Chodźki Str. 20-093 Lublin Poland, annapetruczynik@poczta.onet.pl,
Waksmundzka-Hajnos, M.
Medical University of Lublin Department of Inorganic Chemistry 4a Chodźki Str. 20-093 Lublin Poland,
Rykowska, I.
Adam Mickiewicz University Faculty of Chemistry Umultowska 89b 61-614 Poznań Poland, obstiwo@amu.edu.pl,
Wasiak, W.
Adam Mickiewicz University Faculty of Chemistry Umultowska 89b 61-614 Poznań Poland,
Inglot, T. W.
Medical University of Lublin Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland,
Gumieniczek, A.
anna.gumieniczek@am.lublin.pl,
Komsta, Ł.
Medical University of Lublin Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland,
Związek, R.
Medical University of Lublin Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland,
Sharaf El Din, M. K.
Mansoura University Department of Analytical Chemistry, Faculty of Pharmacy Mansoura 35516 Egypt,
Ibrahim, F.
Mansoura University Department of Analytical Chemistry, Faculty of Pharmacy Mansoura 35516 Egypt,
Eid, M. I.
Mansoura University Department of Analytical Chemistry, Faculty of Pharmacy Mansoura 35516 Egypt,
Wahba, M. E. K.
Mansoura University Department of Analytical Chemistry, Faculty of Pharmacy Mansoura 35516 Egypt, marywahba2004@yahoo.com,
El-Shaboury, S. R.
Assiut University Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Assiut 71526 Egypt,
Hussein, A. S.
Assiut University Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Assiut 71526 Egypt,
Mohamed, N. A.
Assiut University Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Assiut 71526 Egypt, mohamed_mounir2000@yahoo.com,
El-Sutohy, M. M.
Al-Azhar University, Assiut Branch Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Assiut 71524 Egypt,
Madej, K.
Jagiellonian University Department of Analytical Chemistry, Faculty of Chemistry Ingardena St. 3 30-060 Krakow Poland, madejk@chemia.uj.edu.pl,
Persona, K.
Jagiellonian University Department of Analytical Chemistry, Faculty of Chemistry Ingardena St. 3 30-060 Krakow Poland,
Nizio, M.
Jagiellonian University Department of Analytical Chemistry, Faculty of Chemistry Ingardena St. 3 30-060 Krakow Poland,
Emami, J.
Isfahan University of Medical Sciences Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences Isfahan Iran, emami@pharm.mui.ac.ir,
Rezazadeh, M.
Isfahan University of Medical Sciences Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences Isfahan Iran,
Smolarz, H. D.
Medical University of Lublin Chair and Department of Pharmaceutical Botany Lublin Poland, h.smolarz@am.lublin.pl,
Hałka, A.
Medical University of Lublin Department of Physical Chemistry, Chair of Chemistry Lublin Poland,
Chabros, O.
Medical University of Lublin Chair and Department of Pharmaceutical Botany Lublin Poland,
Dzido, T. H.
Medical University of Lublin Department of Physical Chemistry, Chair of Chemistry Lublin Poland,
Rode, S.
Saraswati Vidya Bhawan’s College of Pharmacy Sonarpada, Dombivli (East) 421 204 Maharashtra India,
Bhujbal, P.
Saraswati Vidya Bhawan’s College of Pharmacy Sonarpada, Dombivli (East) 421 204 Maharashtra India,
Sandhya, P.
Saraswati Vidya Bhawan’s College of Pharmacy Sonarpada, Dombivli (East) 421 204 Maharashtra India, iamsandhyap@yahoo.com,
Zhu, F. X.
Jiangsu Provincial Academy of Chinese Medicine Key Laboratory of New Drug Delivery System of Chinese Meteria Medica No. 100 Shizi Road Nanjing 210028 China,
Jia, X. B.
Jiangsu Provincial Academy of Chinese Medicine Key Laboratory of New Drug Delivery System of Chinese Meteria Medica No. 100 Shizi Road Nanjing 210028 China,
Cai, Y.
Jiangsu Provincial Academy of Chinese Medicine Key Laboratory of New Drug Delivery System of Chinese Meteria Medica No. 100 Shizi Road Nanjing 210028 China,
Chen, Y.
Jiangsu Provincial Academy of Chinese Medicine Key Laboratory of New Drug Delivery System of Chinese Meteria Medica No. 100 Shizi Road Nanjing 210028 China,
Bhope, S. G.
MIDC Ranjangaon Analytical R&D, Tulip Lab Pvt. Ltd. F-20/21 Pune 412220 India, bshrinivas16@gmail.com,
Kuber, V. V.
MIDC Ranjangaon Analytical R&D, Tulip Lab Pvt. Ltd. F-20/21 Pune 412220 India,
Nagore, D. H.
MIDC Ranjangaon Analytical R&D, Tulip Lab Pvt. Ltd. F-20/21 Pune 412220 India,
Gaikwad, P. S.
MIDC Ranjangaon Analytical R&D, Tulip Lab Pvt. Ltd. F-20/21 Pune 412220 India,
Patil, M. J.
Marathwada Mitra Mandal College of Pharmacy Department of Pharmacognosy Pune India,
Abstrakty : A simple, sensitive, and accurate liquid chromatographic method with photodiode array detector was developed for the determination of andrographolide, phyllanthin, and hypophyllanthin. The separation was carried out on a reverse-phase 250 mm × 4.6 mm, 5μ symmetry C8 column (Waters). The gradient was prepared from 0.1% orthophosphoric acid (solvent A) and (1:1) acetonitrile:methanol (solvent B) as mobile phase delivered at a flow rate of 1 mL min-1. A linear behavior was observed between observed peak area response, and concentration of analytes was investigated, with good correlation coefficient. The method established was successfully applied to quantify andrographolide, phyllanthin, and hypophyllanthin from the herbal hepatoprotective formulation.

Słowa kluczowe : HPLC, validation, phyllanthin, hypophyllanthin, andrographolide,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 1
Strony : 159 – 169
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DOI :
Cytuj : Petruczynik, A. ,Waksmundzka-Hajnos, M. ,Rykowska, I. ,Wasiak, W. ,Inglot, T. W. ,Gumieniczek, A. ,Komsta, Ł. ,Związek, R. ,Sharaf El Din, M. K. ,Ibrahim, F. ,Eid, M. I. ,Wahba, M. E. K. ,El-Shaboury, S. R. ,Hussein, A. S. ,Mohamed, N. A. ,El-Sutohy, M. M. ,Madej, K. ,Persona, K. ,Nizio, M. ,Emami, J. ,Rezazadeh, M. ,Smolarz, H. D. ,Hałka, A. ,Chabros, O. ,Dzido, T. H. ,Rode, S. ,Bhujbal, P. ,Sandhya, P. ,Zhu, F. X. ,Jia, X. B. ,Cai, Y. ,Chen, Y. ,Bhope, S. G. ,Kuber, V. V. ,Nagore, D. H. ,Gaikwad, P. S. ,Patil, M. J. , Development and validation of RP-HPLC method for simultaneous analysis of andrographolide, phyllanthin, and hypophyllanthin from herbal hepatoprotective formulation. Acta Chromatographica Vol. 25, no. 1/2013
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