Journal : Acta Chromatographica
Article : Development and validation of a RP-HPLC method for simultaneous determination of salicin and eight flavonoids in leaves of Salix Matsudana Koidz

Authors :
Patil, A. N.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Nirmal, S. A.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India, nirmalsunil@rediffmail.com,
Chavan, A. K.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Zhou, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Xu, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Luan, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Ma, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Gong, Y.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Qin, D.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Pan, C.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Jinlong, M.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Guobin, J.
Dalian Nationalities University Environment and Resources College Dalian 11660 China,
Bo, J.
Dalian Nationalities University Life Science College Dalian 116600 China,
Hua, J.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Montejo, C.
Universidad CEU San Pablo Departamento de Ciencias Farmacéuticas y de la Alimentación, Área de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28668 Madrid Spain, montejo@ceu.es,
Civera, C.
Universidad Complutense de Madrid Departamento de Química Física II, Facultad de Farmacia 28040 Madrid Spain,
Barcia, E.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Negro, S.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Fernández-Carballido, A.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Czauderna, M.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland, mr.czauderna@gmail.com,
Marounek, M.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Duskova, D.
Prague-Uhříněves The Institute of Animal Science Prague Czech Republic,
Kowalczyk, J.
Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition 05-110 Jabłonna Poland,
Konda, R. K.
Hindu College of Pharmacy Department of Pharmaceutical Chemistry Amaravathi Road Guntur Andhrapradesh 522002 India, victory2ravi@yahoo.co.in,
Chandu, B. R.
Donbosco College of Pharmacy Department of Pharmaceutical Sciences Pulladigunta Guntur 522201 India,
Challa, B. R.
Nirmala College of Pharmacy Department of Pharmaceutical Analysis Madras Road Kadapa Andhrapradesh 516002 India, baluchalla_99@yahoo.com,
Chandrasekhar, K. B.
Jawaharlal Nehru Technological University Department of Chemistry Anantapur 515002 India,
Shen, Y.
Jiangsu University School of Pharmacy Zhenjiang China,
Yang, H.
Jiangsu University School of Pharmacy Zhenjiang China,
Xia, G.
Jiangsu University School of Pharmacy Zhenjiang China,
Wang, J.
Jiangsu University School of Pharmacy Zhenjiang China,
Cai, B.
Nanjing University of Chinese Medicine Jiangsu Key Laboratory of Chinese Medicine Processing, SATCM Key Laboratory of Standardization for Chinese Medicine Processing Nanjing China,
Jia, X.
Jiangsu University School of Pharmacy Zhenjiang China, jxiaobin2005@hotmail.com,
Nurulain, S. M.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Kalász, H.
Semmelweis University Department of Pharmacology and Pharmacotherapy Budapest Hungary, huba.kalasz@gmail.com,
Szegi, P.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Kuca, K.
University Hospital Biomedical Research Center Hradec Kralove Czech Republic,
Adem, A.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Hasan, M. Y. B.
United Arab Emirates University Department of Pharmacology and Pharmacotherapy Al Ain United Arab Emirates,
Hashemi, F.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Tekes, K.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Sangoi, M. S.
Federal University of Rio Grande do Sul Faculty of Pharmacy 90610-000 Porto Alegre-RS Brazil, maxsilvasangoi@yahoo.com.br,
Wrasse-Sangoi, M.
Federal University of Santa Maria Department of Industrial Pharmacy 97105-900 Santa Maria-RS Brazil,
Hurtado, F. K.
Federal University of Rio Grande do Sul Faculty of Pharmacy 90610-000 Porto Alegre-RS Brazil,
Rolim, C. M. B.
Federal University of Santa Maria Department of Industrial Pharmacy 97105-900 Santa Maria-RS Brazil,
Walorczyk, S.
Institute of Plant Protection — National Research Institute Department of Pesticide Residue Research Władysława Węgorka 20 60-318 Poznań Poland, s_walorczyk@tlen.pl,
Li, W.
Jilin Agricultural University College of Chinese Medicinal Material Changchun 130118 China,
Shi, L. L.
Jilin Agricultural University College of Chinese Medicinal Material Changchun 130118 China,
Han, L. Q.
Jilin Agricultural University College of Chinese Medicinal Material Changchun 130118 China,
Zhang, J.
Jilin Agricultural University College of Chinese Medicinal Material Changchun 130118 China, zhjing0701@163.com,
Abstract : A reversed-phase high-performance liquid chromatographic method was developed for the first time to simultaneously determine salicin and eight flavonoids in leaves of Salix matsudana, that is salicin, luteolin-7-O-glucoside, myricetin, apigenin-3′-oxyethyl-7-O-glucoside, rutin, quercetin, luteolin, kaempferol and apigenin. The separation of these compounds was achieved on a reversed phase C18 column (250 × 4.6 mm, 5 μm), with linear gradient of methanol in 0.2% phosphoric acid solution with a flow rate of 1.0 mL/min with UV detection at 246 nm. The calibration curves for the determination of all analytes showed good linearity over the investigated ranges (r > 0.999). The % relative standard deviation (% RSD) values were less than 0.34%, and the recoveries were between 95.79% and 99.94%. The values of luteolin-7-O-glucoside, salicin, myricetin, apigenin-3′-oxyethyl-7-O-glucoside, rutin, quercetin, luteolin, kaempferol, and apigenin were 1.0 μg g-1, 20.0 μg g-1, 32.9 μg g-1, 2.0 μg g-1, 29.5 μg g-1, 6.0 μg g-1, 1.0 μg g-1, 3.5 μg g-1, and apigenin was not found in the sample. This developed method can be used for evaluating the quality of different plant materials.

Keywords : high performance liquid chromatography (HPLC), quality control, salicin and flavonoid, Salix matsudana,
Publishing house : University of Silesia in Katowice
Publication date : 2013
Number : Vol. 25, no. 4
Page : 735 – 743

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DOI :
Qute : Patil, A. N. ,Nirmal, S. A. ,Chavan, A. K. ,Zhou, L. ,Xu, J. ,Luan, L. ,Ma, J. ,Gong, Y. ,Qin, D. ,Pan, C. ,Jinlong, M. ,Guobin, J. ,Bo, J. ,Hua, J. ,Montejo, C. ,Civera, C. ,Barcia, E. ,Negro, S. ,Fernández-Carballido, A. ,Czauderna, M. ,Marounek, M. ,Duskova, D. ,Kowalczyk, J. ,Konda, R. K. ,Chandu, B. R. ,Challa, B. R. ,Chandrasekhar, K. B. ,Shen, Y. ,Yang, H. ,Xia, G. ,Wang, J. ,Cai, B. ,Jia, X. ,Nurulain, S. M. ,Kalász, H. ,Szegi, P. ,Kuca, K. ,Adem, A. ,Hasan, M. Y. B. ,Hashemi, F. ,Tekes, K. ,Sangoi, M. S. ,Wrasse-Sangoi, M. ,Hurtado, F. K. ,Rolim, C. M. B. ,Walorczyk, S. ,Li, W. ,Shi, L. L. ,Han, L. Q. ,Zhang, J. ,Zhang, J. , Development and validation of a RP-HPLC method for simultaneous determination of salicin and eight flavonoids in leaves of Salix Matsudana Koidz. Acta Chromatographica Vol. 25, no. 4/2013
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