Journal : Acta Chromatographica
Article : Development and validation of a gradient elution high-performance liquid chromatographic method for determination of talinolol in rat plasma: Application to a preclinical food-drug interaction study

Authors :
Gwarda, R. Ł.
Medical University of Lublin Department of Physical Chemistry, Chair of Chemistry 4a Chodźki Street 20-093 Lublin Poland, radoslaw.gwarda@umlub.pl,,
Tomczyszyn, A.
University of Warsaw Faculty of Chemistry 1 Pasteura Street 02-093 Warsaw Poland,
Misicka, A.
University of Warsaw Faculty of Chemistry 1 Pasteura Street 02-093 Warsaw Poland,
Dzido, T. H.
Medical University of Lublin Department of Physical Chemistry, Chair of Chemistry 4a Chodźki Street 20-093 Lublin Poland,
Xue, X. D.
Yunnan Normal University Department of Chemistry Kunming 650092 P.R. China,
Zhang, M.
East China Normal University Department of Chemistry Shanghai 200241 P.R. China,
Xie, S. M.
Yunnan Normal University Department of Chemistry Kunming 650092 P.R. China,
Yuan, L. M.
Yunnan Normal University Department of Chemistry Kunming 650092 P.R. China, yuan_limingpd@yahoo.com.cn,
Matta, M. K.
Jawaharlal Nehru Technological University University College of Pharmaceutical Sciences Hyderabad 500 085 India,
Pilli, N. R.
Jawaharlal Nehru Technological University University College of Pharmaceutical Sciences Hyderabad 500 085 India,
Rao, J. V. L. N. S.
Yalamarty College of Pharmaceutical Sciences Visakhapatnam 530 052 India, jvlnsrao@rediffmail.com,
Abdelhay, M. H.
University of Alexandria Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Elmessalah, Alexandria 21521 Egypt,
Gazy, A. A.
University of Alexandria Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Elmessalah, Alexandria 21521 Egypt,
Shaalan, R. A.
rasha_shaalan@yahoo.com,
Ashour, H. K.
University of Alexandria Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Elmessalah, Alexandria 21521 Egypt,
Krasucka, D.
National Veterinary Research Institute — National Research Institute Department of Veterinary Pharmacy Al. Partyzantów 57 24-100 Pulawy Poland,
Kowalski, C. J.
University of Life Sciences in Lublin Department of Pharmacology, Faculty of Veterinary Medicine Akademicka 12 20-033 Lublin Poland,
Osypiuk, M.
University of Life Sciences in Lublin Department of Pharmacology, Faculty of Veterinary Medicine Akademicka 12 20-033 Lublin Poland, monika.osypiuk@up.lublin.pl,
Opielak, G.
Medical University School of Lublin Human Anatomy Department Jaczewskiego 4 20-090 Lublin Poland,
Mahgoub, H.
King Abdulaziz University Department of Pharmaceutical Chemistry, Faculty of Pharmacy Jeddah Saudi Arabia, hodamahgoub@yahoo.com,
Hanafy, A.
King Abdulaziz University Department of Pharmacology and Toxicology, Faculty of Pharmacy Jeddah Saudi Arabia,
Bamane, F. H.
King Abdulaziz University Department of Biochemistry, Faculty of Medicine Jeddah Saudi Arabia,
Radwan, A. E.
University of Alexandria Department of Biotechnology, Institute of Graduate Studies and Research Alexandria Egypt,
Abstract : A sensitive and reproducible high-performance liquid chromatographic (HPLC) method for determination of talinolol (TAL) in rat plasma was developed and validated using pindolol (PIN) as an internal standard. Both TAL and PIN were separated on a Zorbax Eclipse XDB C18 column by gradient elution with 0.1% aqueous formic acid and acetonitrile as the mobile phase. Detection was performed using fluorescence measurement at λex 249 nm and λem 333 nm. The method was validated and found to be linear in the range of 10–2000 ng mL−1. The limit of quantification was 10 ng mL−1 based on 100 μL of plasma. The variations for intra- and inter-day precision were less than 10%, and the accuracy values were between 92% and 102.9%. The extraction recoveries were more than 82%. The assay was successfully applied to an in-vivo pharmacokinetic study of TAL in rats that were administered a single oral dose of 10 mg kg−1 TAL. The maximum concentration (Cmax) and the area under the plasma concentration-time curve (AUC0–12) were 0.369 ± 0.024 μg mL−1 and 1.429 ± 0.027 μg h mL−1, respectively. The modulatory effect of apricot juice on P-glycoprotein-related efflux carriers was also investigated. Co-administration of apricot juice resulted in a significant increase of the amount of TAL in plasma (Cmax and AUC0–12 were 0.679 ± 0.021 and 2.357 ± 0.079, respectively; p < 0.05).
Keywords : talinolol, HPLC, gradient elution, rat plasma, pharmacokinetic study, food-drug interaction, apricot juice, P-gp,
Publishing house : University of Silesia in Katowice
Publication date : 2015
Number : Vol. 27, no. 1
Page : 67 – 79

Bibliography
: 1 B. Trausch, R. Oertel, K. Richter, T. Gramatté, Biopharm Drug Dispos., 16, 403–414 (1995)
2 I. Campeanu, Eur. J. Heart Fail, 3(3), 377–380 (2001)
3 I. Assmann, Curr. Med. Res. Opin., 13, 325–342 (1995)
4 S. Döppenschmitt, P. Langguth, C.G. Regårdh, T.B. Andersson, C. Hilgendorf, and H. Spahn-Langguth, J. Exp. Pharmacol. Ther., 288, 348–357 (1999a)
5 H. Spahn-Langguth, G. Baktir, A. Radschuweit, A. Okyar, B. Terhaag, P. Ader, A. Hanafy, and P. Langguth, Int. J. Clin. Pharmacol. Ther., 36, 16–24 (1998)
6 PL. Carver, D. Fleisher, Y. Zhou, D. Kaul, P. Kazanjian, C. Li, Pharm. Res., 16, 718–724 (1999)
7 D. Fleisher, L. Cheng, Y. Zhou, LH. Pao, A. Karim, Clin. Pharmacokinet., 36, 233–254 (1999)
8 M. Shimizu, Nahrung, 43, S154–S158 (1999)
9 L. Williams, J.A. Davis, and D.T. Lowenthal, Clin. Nutr., 77, 815–829 (1993)
10 C.A. Plouzek, H.P. Ciolino, R. Clarke, and G.C. Yeh, Eur. J. Cancer, 35, 1541–1545 (1999)
11 H. Spahn-Langguth and P. Langguth, Eur. J. Pharm Sci., 12, 361–367 (2001)
12 H. Takanaga, A. Ohnishi, S. Yamada, H. Matsuo, S. Morimoto, Y. Shoyama, H. Ohtani, and Y. Sawada, J. Pharmacol. Exp. Ther., 293, 230–236 (2000)
13 S. Deferme, J. Van Gelder, and P. Augustijns, J. Pharm. Pharmacol., 54, 1213–1219 (2002)
14 Y. Mitsunaga, H. Takanaga, H. Matsuo, M. Naito, T. Tsuruo, H. Ohtani, and Y. Sawada, Eur. J. Pharmacol., 395, 193–201 (2000)
15 A.B. Shapiro, V. Ling, Biochem. Pharmacol., 53, 587–596 (1997)
16 J. Bharathi, N. Vaidya, and T. Walle, JPET, 307, 745–752 (2003)
17 N.C. Cooks and S. Samman, J. Nutr. Biochem., 7, 66 (1996)
18 J. Van Gelder, S. Deferme, P. Annaert, L. Naesens, E. De Clercq, G. Van den Mooter, R. Kinget, and P. Augustijns, Drug Metab. Dispos., 28, 394–1396 (2000)
19 S.M. Pathak, P.B. Musmade, K.M. Bhat, and N. Udupa, Bioanalysis, 2, 95–104 (2010)
20 P. Augustijns and R. Mols, J. Pharm. Biomed. Anal., 34, 971–978 (2004)
21 H. Juan, B. Terhaag, Y. Li-Ying, Z. Bi-Kui, S. Fen-Li, Z. Yun-Gui, S. Juan, T. Jing, L. Xiao-Lei, and P. Wen-Xing, J. Chromatogr., B, 853, 275–280 (2007)
22 Y. Li, Y. Shirasaka, P. Langguth, and I. Tamai, J. Chromatogr. Sci., 48, 367–370 (2010)
23 International Conference on Harmonization, Geneva, Validation of Analytical Procedures: Text and Methodology, Q2 (R1), EMEA (2006)
24 S. Deferme, R. Mols, W. Driessche, and P. Augustijns, J. Pharm. Sci., 91, 2539–2548 (2002)
25 I. Ameer and R.A. Weintraub, Clin. Pharmacokinet., 33, 103–121 (1997)
DOI :
Qute : Gwarda, R. Ł. ,Tomczyszyn, A. ,Misicka, A. ,Dzido, T. H. ,Xue, X. D. ,Zhang, M. ,Xie, S. M. ,Yuan, L. M. ,Matta, M. K. ,Pilli, N. R. ,Rao, J. V. L. N. S. ,Abdelhay, M. H. ,Gazy, A. A. ,Shaalan, R. A. ,Ashour, H. K. ,Krasucka, D. ,Kowalski, C. J. ,Osypiuk, M. ,Opielak, G. ,Mahgoub, H. ,Hanafy, A. ,Bamane, F. H. ,Radwan, A. E. ,Radwan, A. E. , Development and validation of a gradient elution high-performance liquid chromatographic method for determination of talinolol in rat plasma: Application to a preclinical food-drug interaction study. Acta Chromatographica Vol. 27, no. 1/2015
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