Development and validation of a reversed-phase liquid chromatography method for the quantification of meloxicam in liposomes

Czasopismo : Acta Chromatographica
Tytuł artykułu : Development and validation of a reversed-phase liquid chromatography method for the quantification of meloxicam in liposomes

Autorzy :
Patil, A. N.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Nirmal, S. A.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India, nirmalsunil@rediffmail.com,
Chavan, A. K.
Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India,
Zhou, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Xu, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Luan, L.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Ma, J.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Gong, Y.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Qin, D.
Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China,
Pan, C.
China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China,
Jinlong, M.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Guobin, J.
Dalian Nationalities University Environment and Resources College Dalian 11660 China,
Bo, J.
Dalian Nationalities University Life Science College Dalian 116600 China,
Hua, J.
Zhejiang University Department of Chemical and Biological Engineering Hangzhou 310027 China,
Montejo, C.
Universidad CEU San Pablo Departamento de Ciencias Farmacéuticas y de la Alimentación, Área de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28668 Madrid Spain, montejo@ceu.es,
Civera, C.
Universidad Complutense de Madrid Departamento de Química Física II, Facultad de Farmacia 28040 Madrid Spain,
Barcia, E.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Negro, S.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Fernández-Carballido, A.
Universidad Complutense de Madrid Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia 28040 Madrid Spain,
Abstrakty : In the present study, we have developed and validated an analytical method for the determination of meloxicam in liposomes using high-performance liquid chromatography-ultraviolet (HPLC-UV). Chromatographic separation was carried out on an Ascentis RP amide C16 column selecting a mobile phase composed of acetonitrile-0.3% formic acid solution (40:60, v/v) adjusted at pH 2.8. The mobile phase flow rate selected was 0.5 mL min-1and UV detection at 355 nm. Piroxicam was chosen as internal standard. All the analyses were performed at temperatures of 40.0 ± 0.5°C. The calibration curve was linear over the range 18–420 ng mL-1. Relative standard deviation (RSD) for precision was <1.03%. Accuracy ranged between 98.53% and 101.41% with RSD lower than 1.5%. LOD and LOQ were 5 ng mL-1and 15 ng mL-1, respectively. The method was simple, rapid, and easy to apply, making it very suitable for routine analysis of meloxicam in liposomes. The method could also be used with reliability for the determination of meloxicam in other pharmaceutical dosage forms.

Słowa kluczowe : HPLC, liposomes, meloxicam, UV detection,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2013
Numer : Vol. 25, no. 4
Strony : 639 – 653
Bibliografia : 1 R.N. Rao, S. Meena, and A.R. Rao, J. Pharm. Biomed. Anal., 39, 349–363 (2005)
2 N.B. Naidu, K.P Chowdary, KVR Murthy, V. Satyanarayana, A.R. Hayman, and G. Becket, J. Pharm. Biomed. Anal., 35, 75–86 (2004)
3 A.P. Goldman, C.S. Williams, H.M. Sheng, L.W. Lamps, V.P. Williams, M. Pairet, J.D. Morrow, and R.N. DuBois, Carcinogenesis, 19, 2195–2199 (1998)
4 D. Turck, W. Roth, and U. Busch, Brit. J. Rheumatol., 35, 13–19 (1996)
5 H.J. Hussey and M.J. Tisdale, Int. J. Cancer, 87, 95–100 (2000)
6 Y. Tsubouchi, S. Mukai, Y. Kawahito, R. Yamada, M. Cono, K. Inoue, and H. Sano, Anticancer Res., 20, 2867–2872 (2000)
7 T. Naruse, Y. Nishida, K Hosono, and N. Ishiguro, Carcinogenesis, 27, 584–592 (2006)
8 T. Velpandian, J. Jaiswal, R.K. Bhardwaj, and S.K. Gupta, J. Chromatogr. B Biomed. Sci. Appl., 738, 431–436 (2000)
9 B. Dasandi, H. Shivaprakash, H, Saroj, and K.M. Bhat, J. Pharm. Biomed. Anal., 28, 999–1004 (2002)
10 J.W. Bae, M.J. Kim, C.G. Jang, and S.Y. Lee, J. Chromatogr. B, 859, 69–73 (2007)
11 A. Medvedovici, F. Albu, C. Georgita, C. Mircioiu, and V. David, Arzneimittelforschung, 55, 326–331 (2005)
12 J.L. Wiesner, A.D. de Jager, F.C. Sutherland, H.K. Hundt, K.J. Swart, and A.F. Hundt, J. Els, J. Chromatogr. B, 785, 115–121(2003)
13 H.M. Rigato, G.D. Mendes, N.C. Borges, and R.A. Moreno, Int. J. Clin. Pharmacol. Ther., 44, 489–498 (2006)
14 Y. Yuan, X. Chen, and D. Zhong, J. Chromatogr. B, 852, 650–654 (2007)
15 A. Madni, M. Ahmad, M. Usman, M.M. Zubair, M. Qamar-Uz-Zaman, H.M Shoaib, A. Munir, S.A. Khan, M.N. Amir, and M.S. Qureshi, J. Chem. Soc. Pak., 32, 654–661 (2012)
16 A.D. Bangham, M.M. Standish, and J.C. Watkins, J. Mol. Biol., 13, 238–252 (1965)
17 International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH), Q2B, Guideline on Validation of Analytical Procedure-Methodology, London, 1996
DOI :
Cytuj : Patil, A. N. ,Nirmal, S. A. ,Chavan, A. K. ,Zhou, L. ,Xu, J. ,Luan, L. ,Ma, J. ,Gong, Y. ,Qin, D. ,Pan, C. ,Jinlong, M. ,Guobin, J. ,Bo, J. ,Hua, J. ,Montejo, C. ,Civera, C. ,Barcia, E. ,Negro, S. ,Fernández-Carballido, A. , Development and validation of a reversed-phase liquid chromatography method for the quantification of meloxicam in liposomes. Acta Chromatographica Vol. 25, no. 4/2013
facebook