Development and validation of an HPLC-UV-MS-MS method for identification and quantification of polyphenols in Artemisia pallens L.

Czasopismo : Acta Chromatographica
Tytuł artykułu : Development and validation of an HPLC-UV-MS-MS method for identification and quantification of polyphenols in Artemisia pallens L.

Autorzy :
Turło, J.
Medical University of Warsaw Department of Drug Technology 1 Banacha Street 02-097 Warsaw Poland Medical University of Warsaw Faculty of Pharmacy 1 Banacha Street 02-097 Warsaw Poland, jadwiga.turlo@wum.edu.p,
Komsta, Ł.
Medical University of Lublin Chair and Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland Medical University of Lublin Chair and Department of Pharmaceutical Botany, Faculty of Pharmacy Chodźki 1 20-093 Lublin Pola, lukasz.komsta@am.lublin.pl,
Vasta, J. D.
Lafayette College Department of Chemistry Easton PA 18042-1782 USA Lafayette College Department of Biology Easton PA 18042-1782 USA, shermaj@mail.lafayette.edu,
Sajewicz, M.
Silesian University Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland, kowalska@us.edu.pl,
Raju, Ch. B. V. N.
Aurobindo Pharma Limited Srikakulam Dist. Pydibhimavaram 532 409 India Andhra University Department of Inorganic and Analytical Chemistry Visakhapatnam 530 003 India, chbvn_5@yahoo.co.in,
Shende, P.
NMIMS University School of Pharmacy and Technology Management V.L. Mehta Road, Vile Parle (W) Mumbai 400056 India, shendepravin94@yahoo.co.in,
Dhaneshwar, S. R.
Bharati Vidyapeeth University, Poona College of Pharmacy Department of Pharmaceutical Chemistry Pune 411038 India, sunil.dhaneshwar@gmail.com,
Thakur, A. K.
University Institute of Chemical Technology 1A-286, Medicinal Natural Products Research Laboratory, Pharmaceutical Division Nathalal Parikh Marg, Matunga Mumbai 400 019 Maharastra India Pharmanza Herbal Pvt. Ltd Plot no 214, Near Vadadia Patiya, Borsad-T, achalthakur@gmail.com,
Niranjan, A.
National Botanical Research Institute (Council of Scientific and Industrial Research) Central Instrumentation Facility Lucknow 226 001 India, lehrinbri@yahoo.com,
Abstrakty : Artemisia pallens L. (Compositae) is used in Indian traditional medicine to treat diabetes mellitus, jaundice, hysteria, body pain, and bacterial and fungal infections. A major cause of a variety of diseases is oxidative stress which is reduced by antioxidants such as polyphenols. These secondary metabolites are generally ubiquitous in plants and extensively used in the pharmaceutical, cosmetic, and food industries. In this study a simple and sensitive HPLC-UV-MS-MS-based method was developed for separation, identification, and quantification of polyphenols, for example gallic, protocatechuic, chlorogenic, caffeic, and ferulic acids, rutin, quercetin, and kaempferol. Amounts of polyphenols detected in 50% methanol-water extracts of the plant varied from 0.005% (kaempferol) to 0.24% (protocatechuic acid). Separation of the polyphenols was achieved on a reversed-phase C 18 with a mobile phase prepared from 1% aqueous with acetic acid and acetonitrile at a flow rate of 0.6 mL min-1. The phenolic compounds were detected by UV absorption at 254 nm. The method was validated for linearity, accuracy, precision, LOD, LOQ, specificity, selectivity, and compound stability. Results from intra and inter-day validation (n = 6) showed the method was efficient and rapid. The optimized method was applied to extracts of A. pallens for identification and quantification of the polyphenols. The reference standards and their presence in A. pallens were confirmed by mass spectrometry.

Słowa kluczowe : method development, validation, HPLC-UV-MS-MS, polyphenols, Artemisia pallens,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2009
Numer : Vol. 21, no. 1
Strony : 105 – 116
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DOI :
Cytuj : Turło, J. ,Komsta, Ł. ,Vasta, J. D. ,Sajewicz, M. ,Raju, Ch. B. V. N. ,Shende, P. ,Dhaneshwar, S. R. ,Thakur, A. K. ,Niranjan, A. , Development and validation of an HPLC-UV-MS-MS method for identification and quantification of polyphenols in Artemisia pallens L.. Acta Chromatographica Vol. 21, no. 1/2009
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