Journal : Acta Chromatographica
Article : Development and validation of an HPTLC method for analysis of zerumbone, the anticancer marker from Zingiber zerumbet

Authors :
Komsta, Ł.
Medical University of Lublin Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland, lukasz.komsta@am.lublin.pl,
Zięba, A.
The Medical University of Silesia Department of Organic Chemistry ul. Jagiellońska 4 41-200 Sosnowiec Poland, zieba@edu.sum.pl,
Prus, W.
he University of Bielsko-Biała Institute of Environmental Protection and Engineering ul. Willowa 2 43-309 Bielsko-Biała Poland,
Pacenti, M.
University of Florence Occupational Health Division, Department of Public Health Viale Morgagni 48 50100 Florence Italy,
Dugheri, S.
University of Florence Occupational Health Division, Department of Public Health Viale Morgagni 48 50100 Florence Italy,
Gagliano-Candela, R.
University of Bari Forensic Toxicology Division, Department of Internal Medicine and Public Health P.zza G. Cesare 11 70124 Bari Italy,
Strisciullo, G.
University of Bari Forensic Toxicology Division, Department of Internal Medicine and Public Health P.zza G. Cesare 11 70124 Bari Italy,
Franchi, E.
University of Florence Organic Chemistry Department/ProtEra Srl (Spin Off of Magnetic Resonance Center) Via Lastruccia 13 50019 Sesto Fiorentino, Florence Italy,
Degli Esposti, F.
Chromline Srl Via Anita Garibaldi 40 59100 Prato Italy,
Perchiazzi, N.
Chromline Srl Via Anita Garibaldi 40 59100 Prato Italy,
Boccalon, P.
University of Florence Occupational Health Division, Department of Public Health Viale Morgagni 48 50100 Florence Italy,
Arcangeli, G.
University of Florence Occupational Health Division, Department of Public Health Viale Morgagni 48 50100 Florence Italy,
Cupelli, V.
University of Florence Occupational Health Division, Department of Public Health Viale Morgagni 48 50100 Florence Italy,
Szőke, É.
Semmelweis University Department of Pharmacognosy Budapest Hungary,
Petroianu, G.
Florida International University Department of Cell Biology Miami FL USA,
Tekes, K.
Semmelweis University Department of Pharmacodynamics Nagyvárad tér 4 1089 Budapest Hungary, drtekes@gmail.com,
Benkő, B.
Division of Pharmacology and Drug Safety Richter Gedeon Rt. Budapest Hungary,
Szegi, P.
Semmelweis University Department of Pharmacodynamics Nagyvárad tér 4 1089 Budapest Hungary,
Laufer, R.
Semmelweis University Department of Pharmacology and Pharmacotherapy Budapest Hungary,
Veress, G.
State Cardiology Hospital Balatonfüred Hungary,
Kuśmierek, K.
Military University of Technology Institute of Chemistry 2 Kaliskiego Str. 00-908 Warsaw Poland,
Bald, E.
University of Lodz Department of Environmental Chemistry 163 Pomorska Str. 90-236 Lodz Poland, ebald@uni.lodz.pl,
Ravi, S.
University of Science Malaysia School of Pharmaceutical Sciences 11800 Pulau Penang Malaysia ravigdk2002@yahoo.co.uk, ravigdk2002@yahoo.co.uk,
Darwis, Y.
University of Science Malaysia School of Pharmaceutical Sciences 11800 Pulau Penang Malaysia ravigdk2002@yahoo.co.uk,
Ravanić, N.
University of Belgrade Faculty of Pharmacy Serbia Yugoslavia,
Filipić, S.
University of Belgrade Faculty of Pharmacy Serbia Yugoslavia,
Nikolić, K.
University of Belgrade Faculty of Pharmacy Serbia Yugoslavia,
Popović, G.
University of Belgrade Faculty of Pharmacy Serbia Yugoslavia,
Vovk, I.
National Institute of Chemistry Laboratory for Food Chemistry Ljubljana Slovenia,
Simonovska, B.
National Institute of Chemistry Laboratory for Food Chemistry Ljubljana Slovenia,
Agbaba, D.
University of Belgrade Faculty of Pharmacy Serbia Yugoslavia, danica@pharmacy.bg.ac.yu,
Rout, K. K.
Utkal University Pharmacognosy and Phytochemistry Division, University Department of Pharmaceutical Sciences Bhubaneswar 751004 Orissa India, kd_rout@yahoo.co.in,
Mishra, S. K.
Utkal University Pharmacognosy and Phytochemistry Division, University Department of Pharmaceutical Sciences Bhubaneswar 751004 Orissa India,
Sherma, J.
Lafayette College Department of Chemistry Easton PA 18042-1782 USA,
Abstract : A simple, accurate, and rapid high-performance thin-layer chromatographic (HPTLC) method for quantification of the anticancer marker zerumbone in different parts of Zingiber zerumbet Smith has been established and validated. Chromatography was performed on aluminium foil-backed silica gel 60 F 254 HPTLC plates with the binary mobile phase ethyl acetate-hexane 1.5:8.5. Ultraviolet detection was performed densitometrically at the maximum absorbance wavelength, 250 nm. Regression analysis data for the calibration plot showed there was a linear relationship between response and concentration in the range 60–260 ng with a regression coefficient of 0.9997. The limits of detection and quantification were 20 and 60 ng, respectively. The instrumental precision and repeatability of the method were found to be 0.80 and 1.08%, respectively. Recovery ranging from 97.85 to 100.12% indicated the excellent accuracy of the method. The developed HPTLC method was successfully used for analysis of the marker in different parts of Z. zerumbet , and the maximum zerumbone content (1.81%) was found in the rhizome.

Keywords : high-performance thin-layer chromatography, HPTLC, densitometry, anticancer marker, zerumbone, Zingiber zerumbet,
Publishing house : University of Silesia in Katowice
Publication date : 2009
Number : Vol. 21, no. 3
Page : 443 – 452

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DOI :
Qute : Komsta, Ł. ,Zięba, A. ,Prus, W. ,Pacenti, M. ,Dugheri, S. ,Gagliano-Candela, R. ,Strisciullo, G. ,Franchi, E. ,Degli Esposti, F. ,Perchiazzi, N. ,Boccalon, P. ,Arcangeli, G. ,Cupelli, V. ,Szőke, É. ,Petroianu, G. ,Tekes, K. ,Benkő, B. ,Szegi, P. ,Laufer, R. ,Veress, G. ,Kuśmierek, K. ,Bald, E. ,Ravi, S. ,Darwis, Y. ,Ravanić, N. ,Filipić, S. ,Nikolić, K. ,Popović, G. ,Vovk, I. ,Simonovska, B. ,Agbaba, D. ,Rout, K. K. ,Mishra, S. K. ,Sherma, J. ,Sherma, J. , Development and validation of an HPTLC method for analysis of zerumbone, the anticancer marker from Zingiber zerumbet. Acta Chromatographica Vol. 21, no. 3/2009
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