Development and validation of an HPTLC method for determination of minocycline in human plasma

Czasopismo : Acta Chromatographica
Tytuł artykułu : Development and validation of an HPTLC method for determination of minocycline in human plasma

Autorzy :
Sherma, J.
Department of Chemistry, Lafayette College, Easton, PA 18042, USA,
Vrchotová, N.
Institute of Systems Biology and Ecology AS CR, Branišovská 31, 370 05 České Budějovice, Czech Republic,
Sivakumar, T.
Department of Pharmacy, Faculty of Engineering and Technology, Annamalai University, Annamalainagar, Tamil Nadu-608 002, India,
Petruczynik, A.
Department of Inorganic Chemistry, Medical University, Staszica 6, 20-081 Lublin, Poland,
Benkő, B.
PhD School of Semmelweis University, 1085 Budapest, Üllői út 26, Hungary, Division of Pharmacology and Drug Safety, Richter Gedeon Rt., Gyömrői út 21, 1475 Budapest, P.O. Box 27, Hungary, Department of Chemistry and Biochemistry, University of Pécs, Péc,
Mori, M.
Department of Chemistry, Faculty of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu, 376-8515, Japan,
Solangi, A. R.
National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan,
Idris, A. M.
Department of Chemistry, College of Science, King Faisal University, Hofuf 31982, Saudi Arabia,
Milán-Segovia, R.
Laboratorio de Farmacia, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Ave. Manuel Nava No. 6, 78290 San Luis Potosí, S.L.P., Mexico,
Qutab, S. S.
Schazoo Laboratories (Pvt) Ltd, 45 G.T. Road, Lahore-54000, Pakistan,
Agil, M.
Faculty of Pharmacy, Hamdard University New Delhi, 110 062, India,
Dhaneshwar, S. S.
Department of Pharmaceutical Chemistry, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, Pune-411 038, India,
Gannu, R.
Centre for Biopharmaceutics and Pharmacokinetics, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Andhra Pradesh, India,
Popović, G.
Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, P.O. Box 146, 11000 Belgrade, Serbia,
Mhaske, D. V.
Department of Quality Assurance Techniques and Pharmaceutical Chemistry, Bharati Vidyapeeth University, Poona College of Pharmacy, Centre for Advanced Pharmaceutical Research, Erandwane, Pune 411038, Maharashtra State, India,
Mohammad, A.
Analytical Research Laboratory, Department of Applied Chemistry, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh 202002, India,
Jain, G. K.
Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi, 110062, India,
Abstrakty : A new high-performance thin-layer chromatographic (HPTLC) method has been established for determination of minocycline in human plasma. Chromatography was performed on aluminium plates coated with silica gel 60F254; the mobile phase was methanol–acetonitrile–isopropanol–water 5:4:0.5:0.5 (v/v). Densitometric analysis was performed at 345 nm. The method is rapid (single-step extraction with methanol), sensitive (limit of quantification 15.4 ng per zone), precise (CV ≤ 4.61 %), accurate (drug recovery 95.08–100.6%), and linear over the range 100–1200 ng per zone. Recovery of minocycline from plasma samples was 95.8 ± 4.5%. The halflife of minocycline in plasma was 9.9 h at 4°C and 6.3 h at 20°C. Minocycline is stable in human plasma for at least two months at -20°C and can tolerate two freeze–thaw cycles with losses <10%. The method was successfully used to determine therapeutic levels of minocycline.
Słowa kluczowe :
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2007
Numer : No. 19
Strony : 197 – 205
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DOI :
Cytuj : Sherma, J. ,Vrchotová, N. ,Sivakumar, T. ,Petruczynik, A. ,Benkő, B. ,Mori, M. ,Solangi, A. R. ,Idris, A. M. ,Milán-Segovia, R. ,Qutab, S. S. ,Agil, M. ,Dhaneshwar, S. S. ,Gannu, R. ,Popović, G. ,Mhaske, D. V. ,Mohammad, A. ,Jain, G. K. , Development and validation of an HPTLC method for determination of minocycline in human plasma. Acta Chromatographica No. 19/2007
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