Journal : Acta Chromatographica
Article : Development and validation of an HPTLC-densitometric method for the estimation of trigonelline in the leaves of Abrus precatorius L. and its formulation

Authors :
Sajewicz, M.
University of Silesia Department of General Chemistry and Chromatography 9 Szkolna Street 40-006 Katowice Poland,
Kowalska, T.
University of Silesia Department of General Chemistry and Chromatography 9 Szkolna Street 40-006 Katowice Poland, teresa.kowalska@us.edu.pl,
Szalka, M.
ICN Polfa Rzeszów S.A. Product Development Department 35-959 Rzeszów Poland,
Kostka, J.
Rzeszów University of Technology Department of Chemical and Process Engineering 35-959 Rzeszów,
Rokaszewski, E.
ICN Polfa Rzeszów S.A. Product Development Department 35-959 Rzeszów Poland,
Kaczmarski, K.
Rzeszów University of Technology Department of Chemical and Process Engineering 35-959 Rzeszów, kkaczmarski@prz.edu.pl,
Belal, F.
University of Mansoura Department of Analytical Chemistry, Faculty of Pharmacy 35516 Mansoura Egypt,
El-Brashy, A. M.
University of Mansoura Department of Analytical Chemistry, Faculty of Pharmacy 35516 Mansoura Egypt,
El-Enany, N.
University of Mansoura Department of Analytical Chemistry, Faculty of Pharmacy 35516 Mansoura Egypt,
Tolba, M. M.
University of Mansoura Department of Analytical Chemistry, Faculty of Pharmacy 35516 Mansoura Egypt,
Cielecka-Piontek, J.
Poznan University of Medical Sciences Department of Pharmaceutical Chemistry, Faculty of Pharmacy Grunwaldzka 6 60-780 Poznań Poland, jpiontek@ump.edu.pl,
Krause, A.
PozLab Contract Research Organization Poznań Poland,
Zalewski, P.
Poznan University of Medical Sciences Department of Pharmaceutical Chemistry, Faculty of Pharmacy Grunwaldzka 6 60-780 Poznań Poland,
Lunzer, A.
Tanaka Laboratory in Hokkaido University W 7, Kita-ku Sapporo 060-8638 Japan,
Jelińska, A.
Poznan University of Medical Sciences Department of Pharmaceutical Chemistry, Faculty of Pharmacy Grunwaldzka 6 60-780 Poznań Poland,
Sarju, N.
Universiti Teknologi Malaysia Department of Industrial Biotechnology, Faculty of Biosciences and Bioengineering 81310 Skudai, Johor Bahru, Johor Malaysia,
Abd Samad, A.
Universiti Teknologi Malaysia Department of Industrial Biotechnology, Faculty of Biosciences and Bioengineering 81310 Skudai, Johor Bahru, Johor Malaysia,
Abd Ghani, M.
Universiti Kebangsaan Malaysia School of Chemical Sciences and Food Technology 43600 UKM Bangi, Selangor Malaysia,
Ahmad, F.
Universiti Teknologi Malaysia Department of Chemistry, Faculty of Science 81310 Skudai, Johor Bahru, Johor Malaysia, farediah@kimia.fs.utm.my,
Yang, G. J.
China Pharmaceutical University School of Pharmacy Nanjing 210009 P. R. China, yanggongjun888@163.com,
Lv, L.
Yangzhou University College of Chemistry and Chemical Engineering Yangzhou 225002 P. R. China,
O’sullivan, C.
Lafayette College Department of Chemistry Easton PA 18042 USA,
Sherma, J.
Lafayette College Department of Chemistry Easton PA 18042 USA, shermaj@lafayette.edu,
Nagaraju, P. M.
Forensic Science Laboratory Madivala, Bangalore 560068 Karnataka India,
Sanganalmath, P. U.
Forensic Science Laboratory Madivala, Bangalore 560068 Karnataka India,
Kemparaju, K.
Mysore University Department of Studies in Biochemistry, Manasa Gangothri Mysore Karnataka India,
Mohan, B. M.
Forensic Science Laboratory Madivala, Bangalore 560068 Karnataka India, praveen_usp@yahoo.com,
Kumbhalkar, B. B.
Agharkar Research Institute Botany Group, Plant Science Division G.G. Agarkar Road Pune 411 004 India,
Rajopadhye, A. A.
Agharkar Research Institute Botany Group, Plant Science Division G.G. Agarkar Road Pune 411 004 India,
Upadhye, A. S.
Agharkar Research Institute Botany Group, Plant Science Division G.G. Agarkar Road Pune 411 004 India, upadhye.anuradha@gmail.com,
Abstract : A new, simple, and reproducible HPTLC-densitometric method has been established and validated for estimation of trigonelline in the leaves of Abrus precatorius L. and its herbal formulation Chatak® using ICH guidelines. Accelerated solvent extraction (ASE) as an alternative to conventional techniques was also explored for rapid extraction. The methanol extracts of leaves, its formulation, and standard solution of trigonelline were applied on silica gel F254 HPTLC plates and developed in a twin chamber using the mobile phase toluene-ethyl acetate-formic acid-methanol (2:3:1.8:3.8, υ/υ/υ/υ). The plates were scanned at 268 nm (λmax of trigonelline) using a Camag TLC scanner 3 with the CATS 4 software. A linear relationship was obtained between the response (peak area) and the amount of trigonelline in the range 40–200 ng per spot; the correlation coefficient was 0.9957. The ASE method gives higher extraction efficiency in less time compared to Soxhlet extraction. The HPTLC-densitometric method showed good linearity, recovery, and high precision. It is useful to analyze trigonelline in A. precatorius leaves and routine quality control of its marketed formulation.

Keywords : trigonelline, Abrus precatorius L, accelerated solvent extraction, HPTLC-densitometry, ICH guidelines,
Publishing house : University of Silesia in Katowice
Publication date : 2012
Number : Vol. 24, no. 2
Page : 263 – 273

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DOI :
Qute : Sajewicz, M. ,Kowalska, T. ,Szalka, M. ,Kostka, J. ,Rokaszewski, E. ,Kaczmarski, K. ,Belal, F. ,El-Brashy, A. M. ,El-Enany, N. ,Tolba, M. M. ,Cielecka-Piontek, J. ,Krause, A. ,Zalewski, P. ,Lunzer, A. ,Jelińska, A. ,Sarju, N. ,Abd Samad, A. ,Abd Ghani, M. ,Ahmad, F. ,Yang, G. J. ,Lv, L. ,O’sullivan, C. ,Sherma, J. ,Nagaraju, P. M. ,Sanganalmath, P. U. ,Kemparaju, K. ,Mohan, B. M. ,Kumbhalkar, B. B. ,Rajopadhye, A. A. ,Upadhye, A. S. ,Upadhye, A. S. , Development and validation of an HPTLC-densitometric method for the estimation of trigonelline in the leaves of Abrus precatorius L. and its formulation. Acta Chromatographica Vol. 24, no. 2/2012
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