Journal : Acta Chromatographica
Article : Development and validation of high-performance thin-layer chromatography method for determination of vicine in herbal extract and formulation

Authors :
Fatemi, M. H.
University of Mazandaran Faculty of Chemistry Babolsar Iran,
Hadjmohammadi, M. R.
University of Mazandaran Faculty of Chemistry Babolsar Iran, hdjmr@umz.ac.ir,
Shakeri, P.
University of Mazandaran Faculty of Chemistry Babolsar Iran,
Bhushan, R.
Indian Institute of Technology Roorkee Department of Chemistry Roorkee 247667 India, rbushfcy@iitr.ernet.in,
Lal, M.
Indian Institute of Technology Roorkee Department of Chemistry Roorkee 247667 India,
Thomas, S.
Jubilant Life Sciences Ltd. Analytical Research Department R&D Centre, C-26, Sector-59 Noida Uttar Pradesh 201 301 India, saji_thomas@jubl.com,
Paul, S. K.
Jubilant Life Sciences Ltd. Analytical Research Department R&D Centre, C-26, Sector-59 Noida Uttar Pradesh 201 301 India,
Agarwal, A.
Jubilant Life Sciences Ltd. Analytical Research Department R&D Centre, C-26, Sector-59 Noida Uttar Pradesh 201 301 India,
Mathela, C. S.
Kumaun University Department of Chemistry DSB Campus Nainital Uttarakhand 263 001 India,
Elgizawy, S. M.
Assuit University Analytical Chemistry Department, Faculty of Pharmacy 71515 Assuit Egypt,
Hassan, N. Y.
Cairo University Analytical Chemistry Department, Faculty of Pharmacy Kasr-El Aini Street 11562 Cairo Egypt,
Lotfy, H. M.
Cairo University Analytical Chemistry Department, Faculty of Pharmacy Kasr-El Aini Street 11562 Cairo Egypt,
Saleh, S. S.
October University for Modern Sciences and Arts (MSA) Analytical Chemistry Department, Faculty of Pharmacy 11787 6th October City Egypt, drsarahsalah@gmail.com,
Shakya, A.
Al-Ahliyya Amman University Faculty of Pharmacy and Medical Sciences PO Box 263 Amman 19328 Jordan, ashokshakya@hotmail.com,
Arafat, T.
Petra University Faculty of Pharmacy PO Box 961343 Amman Jordan,
Hakooz, N. M.
Zarqa University Faculty of Pharmacy Zarqa 13132 Jordan,
Abuawwad, A. N.
Jordan Center for Pharmaceutical Research PO Box 950435 Amman 11495 Jordan,
Al-Hroub, H.
Jordan Center for Pharmaceutical Research PO Box 950435 Amman 11495 Jordan,
Melhim, M.
Jordan Center for Pharmaceutical Research PO Box 950435 Amman 11495 Jordan,
Haliński, Ł.
University of Gdańsk Department of Environmental Analysis, Institute for Environmental and Human Health Protection, Faculty of Chemistry Wita Stwosza 63 80-952 Gdańsk Poland, lukaszh@chem.univ.gda.pl,
Śmigiel, D.
University of Gdańsk Department of Environmental Analysis, Institute for Environmental and Human Health Protection, Faculty of Chemistry Wita Stwosza 63 80-952 Gdańsk Poland,
Czerwicka, M.
University of Gdańsk Department of Environmental Analysis, Institute for Environmental and Human Health Protection, Faculty of Chemistry Wita Stwosza 63 80-952 Gdańsk Poland,
Paszkiewicz, M.
University of Gdańsk Department of Environmental Analysis, Institute for Environmental and Human Health Protection, Faculty of Chemistry Wita Stwosza 63 80-952 Gdańsk Poland,
Kumirska, J.
University of Gdańsk Department of Environmental Analysis, Institute for Environmental and Human Health Protection, Faculty of Chemistry Wita Stwosza 63 80-952 Gdańsk Poland,
Stepnowski, P.
University of Gdańsk Department of Environmental Analysis, Institute for Environmental and Human Health Protection, Faculty of Chemistry Wita Stwosza 63 80-952 Gdańsk Poland,
Odeh, I.
Al-Quds University Chemistry and Chemical Technology Department P.O. Box 20002 Abu-Deis Palestine,
Abu-Lafi, S.
Al-Quds University Faculty of Pharmacy P.O. Box 20002 Abu-Deis Palestine, sabulafi@science.alquds.edu,
Al-Najjar, I.
Al-Quds University Chemistry and Chemical Technology Department P.O. Box 20002 Abu-Deis Palestine,
Znini, M.
University My Ismail Laboratoire des Substances Naturelles & Synthèse et Dynamique Moléculaire, Faculté des Sciences et Techniques Errachidia Morocco,
Majidi, L.
University My Ismail Laboratoire des Substances Naturelles & Synthèse et Dynamique Moléculaire, Faculté des Sciences et Techniques Errachidia Morocco, lmajidi@yahoo.fr,,
Desjobert, J. M.
Université de Corse UMR CNRS 6134, Laboratoire de Chimie des Produits Naturels, Faculté des Sciences et Techniques Corse France,
Paolini, J.
Université de Corse UMR CNRS 6134, Laboratoire de Chimie des Produits Naturels, Faculté des Sciences et Techniques Corse France,
Costa, J.
Université de Corse UMR CNRS 6134, Laboratoire de Chimie des Produits Naturels, Faculté des Sciences et Techniques Corse France,
Xie, J.
Beijing University of Chinese Medicine School of Chinese Materia Medica Beijing 100102 China,
Zhang, Y.
Tianjin University of Commerce Department of Pharmaceutical Engineering Tianjin 300134 China,
Wang, W.
Beijing University of Chinese Medicine School of Chinese Materia Medica Beijing 100102 China, wwq57@126.com,
Hou, J.
Beijing University of Chinese Medicine School of Chinese Materia Medica Beijing 100102 China,
Zhu, M.
The First Affiliated Hospital of Nanjing University of Chinese Medicine West Phoenix Street, 150-1 210036 Nanjing China,
Duan, J.
Nanjing University of Chinese Medicine Jiangsu Key Laboratory for High Technology Research of TCM Formulae Xianlin Road 138 210023 Nanjing China,
Tang, Y. P.
Nanjing University of Chinese Medicine Jiangsu Key Laboratory for High Technology Research of TCM Formulae Xianlin Road 138 210023 Nanjing China,
Guo, J. M.
Nanjing University of Chinese Medicine Jiangsu Key Laboratory for High Technology Research of TCM Formulae Xianlin Road 138 210023 Nanjing China,
Shang, E. X.
Nanjing University of Chinese Medicine Jiangsu Key Laboratory for High Technology Research of TCM Formulae Xianlin Road 138 210023 Nanjing China,
Zhu, Z. H.
Nanjing University of Chinese Medicine Jiangsu Key Laboratory for High Technology Research of TCM Formulae Xianlin Road 138 210023 Nanjing China, zhumin0902@163.com,
Wang, T.
Hebei Normal University of Science and Technology Hebei Chestnut Engineering Technology Research Center Qinhuangdao, Hebei 066600 China, trueyeoman2008@yahoo.com.cn,
Yang, Y. D.
Hebei Normal University of Science and Technology Hebei Chestnut Engineering Technology Research Center Qinhuangdao, Hebei 066600 China,
Du, B.
Hebei Normal University of Science and Technology Hebei Chestnut Engineering Technology Research Center Qinhuangdao, Hebei 066600 China, bindufood@yahoo.com.cn,
Yu, S.
Hebei Normal University of Science and Technology Hebei Chestnut Engineering Technology Research Center Qinhuangdao, Hebei 066600 China,
Hou, W. L.
Hebei Normal University of Science and Technology Hebei Chestnut Engineering Technology Research Center Qinhuangdao, Hebei 066600 China,
Bedi, R. S.
ISF College of Pharmacy Department of Pharmacognosy Moga 142001 India,
Gauttam, V. K.
ISF College of Pharmacy Department of Pharmacognosy Moga 142001 India,
Kalia, A. N.
ISF College of Pharmacy Department of Pharmacognosy Moga 142001 India, ankalia_47@rediffmail.com,
Abstract : A simple, selective, precise, and reproducible high-performance thin-layer chromatography (HPTLC) method for the analysis of vicine in herbal extracts and formulations containing Momordica charantia was developed. The study was performed on aluminum-based pre-coated TLC plates (silica gel 60F254). The chromatograms of samples were developed in twin trough glass chamber pre-saturated with mobile phase comprised of ethyl acetate-methanol-water-formic acid (7.5:3:1:0.1, v/v/v/v) at room temperature (25 ± 2°C). The densitometric analysis was carried out in absorbance mode at 278 nm. The optimized mobile phase showed a compact spot of vicine (RF = 0.31 ± 0.02) from the other constituents present in the samples. The linear regression analysis data for the calibration plots showed good linearity (r2 = 0.9990) with respect to peak area in the range of 200–1400 ng spot-1. The method was validated as per International Conference on Harmonization (ICH) guidelines. The limits of detection and quantification (50 and 150 ng spot−1, respectively) were also established. The proposed method has shown the excellent recovery (98.01–99.33%), which supports the suitability of the method for the analysis. Statistical analysis of the obtained data showed the selectivity of the proposed method for vicine estimation in the herbal samples.

Keywords : herbal extract, Momordica charantia, validation, vicine,
Publishing house : University of Silesia in Katowice
Publication date : 2014
Number : Vol. 26, no. 3
Page : 551 – 562

Bibliography
: 1 J. K. Grover, and S. P. Yadav, J. Ethnopharmacol., 93, 123 (2004)
2 J. Virdi, S. Sivakami, S. Shahani, A.C. Suthar, M.M. Banavalikar, and M.K. Biyani, J. Ethnopharmacol., 88, 107 (2003)
3 M. Tan, J. Ye, N. Turner, C. Hohnen-Behrens, C. Ke, C. Tang, T. Chen, H. Weiss, E. Gesing, A. Rowland, D. E. James, and Y. Ye, Chem. Biol., 15, 263 (2008)
4 S. Omar, Z. Ansari, and M. Nehal, Fitoterapia, 78, 46 (2007)
5 J. E. Cunnick, K. Sakamoto, S. K. Chapes, G. W. Fortner, and D. J. Takemoto, Cell Immunol., 126, 278 (1990)
6 J. H. Kim, E. M. Ju, D. K. Lee, and H. J. Hwang, Anticancer Res., 22, 1885 (2002)
7 Y. Hwang, J. W. Lee, E. Hahm, K. C. Jung, J. H. Lee, C. H. Park, H. S. Rhee, J. M. Ryu, H. Kim, and C. Yang, Biochem. Biophys. Res. Commun., 337, 815 (2005)
8 I. Ahmed, M. S. Lakhani, M. Gillett, A. John, and H. Raza, Diabetes Res. Clin. Pract., 51, 155 (2001)
9 P. Chaturvedi, Br. J. Biomed. Sci., 62, 124 (2005)
10 P. Nerurkar, Y. K. Lee, E. H. Linden, S. Lim, L. Pearson, J. Frank, and V. R. Nerurkar, Br. J. Pharmacol., 148, 1156 (2006)
11 N. M. Ansari, L. Houlihan, B. Hussain, and A. Pieroni, Phytother. Res., 19, 907 (2005)
12 I. Gurbuz, C. Akyuz, E. Yesilada, and B. Sener, J. Ethnopharmacol., 7, 77 (2000)
13 A. R. Biswas, S. Ramaswamy, and J. S. Bapna, J. Ethnopharmacol., 31, 115 (1991)
14 J. Choi, K. T. Lee, H. Jung, H. S. Park, and H. J. Park, Arch. Pharm. Res., 25, 336 (2002)
15 A. Sharanabasappa, B. Vijayakumar Saraswati, and B. Patil, Pharm. Biol., 40, 501 (2002)
16 V. P. Dixit, P. Khanna, and S. K. Bhargava, Planta Med., 34, 280 (1978)
17 A. Raman, and C. Lau, Phytomedicine, 2, 349 (1996)
18 S. I. Kim, E. Hoehn, N. A. M. Eskin, and F. Ismail, J. Agric. Food Chem., 30, 144 (1982)
19 H. S. Olsen, and J. H. Andersen, J. Sci. Food Agric., 29, 323 (1978)
20 H. Zhang, Y. Wang, X. Zhang, M. Liu, and Z. Hu, Anal. Lett., 36, 1597 (2003)
21 E. A. Abourashed, and J. S. Mossa, J. Pharm. Biomed. Anal., 36, 617 (2004)
22 H. Agarawal, N. Kaul, A. R. Paradkar, and K. R. Mahadik, J. Pharm. Biomed. Anal., 36, 33 (2004)
23 V.B. Patravale, S. D’souza, and Y. Narkar, J. Pharm. Biomed. Anal., 25, 685 (2001)
24 International Conference on Harmonization, Q2A, Geneva, 2005
DOI :
Qute : Fatemi, M. H. ,Hadjmohammadi, M. R. ,Shakeri, P. ,Bhushan, R. ,Lal, M. ,Thomas, S. ,Paul, S. K. ,Agarwal, A. ,Mathela, C. S. ,Elgizawy, S. M. ,Hassan, N. Y. ,Lotfy, H. M. ,Saleh, S. S. ,Shakya, A. ,Arafat, T. ,Hakooz, N. M. ,Abuawwad, A. N. ,Al-Hroub, H. ,Melhim, M. ,Haliński, Ł. ,Śmigiel, D. ,Czerwicka, M. ,Paszkiewicz, M. ,Kumirska, J. ,Stepnowski, P. ,Odeh, I. ,Abu-Lafi, S. ,Al-Najjar, I. ,Znini, M. ,Majidi, L. ,Desjobert, J. M. ,Paolini, J. ,Costa, J. ,Xie, J. ,Zhang, Y. ,Wang, W. ,Hou, J. ,Zhu, M. ,Duan, J. ,Tang, Y. P. ,Guo, J. M. ,Shang, E. X. ,Zhu, Z. H. ,Wang, T. ,Yang, Y. D. ,Du, B. ,Yu, S. ,Hou, W. L. ,Bedi, R. S. ,Gauttam, V. K. ,Kalia, A. N. ,Kalia, A. N. , Development and validation of high-performance thin-layer chromatography method for determination of vicine in herbal extract and formulation. Acta Chromatographica Vol. 26, no. 3/2014
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