Journal : Acta Chromatographica
Article : Development of an HPLC fingerprint for quality control and species differentiation of Uncaria rhynchophylla (Miq.) ex Havil

Authors :
Xu, L. N.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Gai, F. Y.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Mu, G. F.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Gao, Y.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Liu, C. J.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Luan, F.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Mori, M.
Gunma University Department of Chemistry and Chemical Biology, Graduate School of Engineering 1-5-1 Tenjin-cho Kiryu 376-8515 Japan,
Ikeda, T.
Gunma University Department of Chemistry and Chemical Biology, Graduate School of Engineering 1-5-1 Tenjin-cho Kiryu 376-8515 Japan,
Itabashi, H.
Gunma University Department of Chemistry and Chemical Biology, Graduate School of Engineering 1-5-1 Tenjin-cho Kiryu 376-8515 Japan,
Soomro, R.
University of Sindh National Center of Excellence in Analytical Chemistry Jamshoro Pakistan,
Memon, S. Q.
University of Sindh Institute of Advance Research in Chemical Sciences Jamshoro Pakistan,
Ahmed, M. J.
University of Chittagong Laboratory of Analytical Chemistry, Department of Chemistry Chittagong 4331 Bangladesh,
Memon, N.
University of Sindh National Center of Excellence in Analytical Chemistry Jamshoro Pakistan, najmamemon@gmail.com,
Mallah, A.
University of Sindh M.A. Kazi Institute of Chemistry Jamshoro Pakistan,
Ravi, P. R.
BITS-Pilani Hyderabad Campus Pharmacy Department Ranga Reddy (Dist.) Jawaharnagar Andhra Pradesh India, rahulvats1983@gmail.com,
Aditya, N.
BITS-Pilani Hyderabad Campus Pharmacy Department Ranga Reddy (Dist.) Jawaharnagar Andhra Pradesh India, adityamurthy1212@gmail.com,
Vats, R.
BITS-Pilani Hyderabad Campus Pharmacy Department Ranga Reddy (Dist.) Jawaharnagar Andhra Pradesh India, rpunnarao@gmail.com,
Navaneethan, G.
Sona College of Technology (Anna University) Department of Sciences Salem 636 005 India,
Karunakaran, K.
Sona College of Technology (Anna University) Department of Sciences Salem 636 005 India, drkk@sonatech.ac.in,
Elango, K. P.
Gandhigram Rural Institute (Deemed University) Department of Chemistry Gandhigram 624 302 India,
Prichodko, A.
Vilnius University Department of Analytical and Environmental Chemistry Naugarduko 24 LT-03225 Vilnius Lithuania,
Janenaite, E.
Vilnius University Department of Analytical and Environmental Chemistry Naugarduko 24 LT-03225 Vilnius Lithuania,
Smitiene, V.
Vilnius University Department of Analytical and Environmental Chemistry Naugarduko 24 LT-03225 Vilnius Lithuania,
Vickackaite, V.
Vilnius University Department of Analytical and Environmental Chemistry Naugarduko 24 LT-03225 Vilnius Lithuania, vida.vickackaite@chf.vu.lt,
Akhtar, N.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India,
Faiyazuddin, M.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India, md.faiyazuddin@yahoo.co.in,
Mustafa, G.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India,
Sultana, Y.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India,
Baboota, S.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India,
Ali, J.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India,
Wang, D.
Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China,
Bai, A.
inan Municipal Center For Disease Control and Prevention Jinan 250001 P. R. China,
Lin, X.
Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China,
Fang, L.
Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China,
Shu, X.
Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China,
Shi, X.
Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China,
Sun, Q.
Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China,
Wang, X.
Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China, wxjn1998@126.com,
Wu, J.
Xuzhou Medical College Key Laboratory of New Drug and Clinical Application Xuzhou Jiangsu 221004 China,
Xing, H.
Xuzhou Medical College Key Laboratory of New Drug and Clinical Application Xuzhou Jiangsu 221004 China,
Tang, D.
Xuzhou Medical College Key Laboratory of New Drug and Clinical Application Xuzhou Jiangsu 221004 China, tdq993@hotmail.com,
Gao, Y.
Xuzhou Medical College Key Laboratory of New Drug and Clinical Application Xuzhou Jiangsu 221004 China,
Yin, X.
Xuzhou Medical College Key Laboratory of New Drug and Clinical Application Xuzhou Jiangsu 221004 China,
Du, Q.
Xuzhou Medical College Key Laboratory of New Drug and Clinical Application Xuzhou Jiangsu 221004 China,
Jiang, X.
Xuzhou Medical College Key Laboratory of New Drug and Clinical Application Xuzhou Jiangsu 221004 China,
Yang, D.
Xuzhou Medical College Key Laboratory of New Drug and Clinical Application Xuzhou Jiangsu 221004 China,
Lu, W.
Xi’an Jiao tong University School of Medicine Xi’an 710061 P. R. China,
Yang, F.
Xi’an Jiao tong University School of Medicine Xi’an 710061 P. R. China,
Wang, S.
Xi’an Jiao tong University School of Medicine Xi’an 710061 P. R. China, wangsc@mail.xjtu.edu.cn,
Abstract : The stems of Uncaria rhynchophylla (Miq.) ex Havil have a long history of use in traditional Chinese medicine to treat diseases and improve health. There is evident evidence that alkaloids constituents are mainly responsible for the beneficial effects of this plant medicine. The amounts of the major bioactive alkaloids in this plant vary widely with species, habitat, and as such, and establishment of a high-performance liquid chromatography (HPLC) fingerprint for quality control of this herbal medicine is of particular importance. The most alkaloids are used for medicine treatment and research. On the basis of the chromatographic data, a consistent HPLC fingerprint pattern containing 15 common peaks was obtained. Among these common peaks, four were identified as rhynchophylline, isorhynchophylline, corynoxeine, and isocorynoxeine. On the basis of this HPLC fingerprint and principal-components analysis, the quality of fifteen samples from different producing areas of China was objectively assessed. To summarize, the data described in this study offer valuable information for quality control and proper use of U. rhynchophylla (Miq.) ex Havil.

Keywords : Uncaria rhynchophylla (Miq.) ex Havil, HPLC fingerprinting, Similarity Evaluation System,
Publishing house : University of Silesia in Katowice
Publication date : 2012
Number : Vol. 24, no. 4
Page : 643 – 651

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DOI :
Qute : Xu, L. N. ,Gai, F. Y. ,Mu, G. F. ,Gao, Y. ,Liu, C. J. ,Luan, F. ,Mori, M. ,Ikeda, T. ,Itabashi, H. ,Soomro, R. ,Memon, S. Q. ,Ahmed, M. J. ,Memon, N. ,Mallah, A. ,Ravi, P. R. ,Aditya, N. ,Vats, R. ,Navaneethan, G. ,Karunakaran, K. ,Elango, K. P. ,Prichodko, A. ,Janenaite, E. ,Smitiene, V. ,Vickackaite, V. ,Akhtar, N. ,Faiyazuddin, M. ,Mustafa, G. ,Sultana, Y. ,Baboota, S. ,Ali, J. ,Wang, D. ,Bai, A. ,Lin, X. ,Fang, L. ,Shu, X. ,Shi, X. ,Sun, Q. ,Wang, X. ,Wu, J. ,Xing, H. ,Tang, D. ,Gao, Y. ,Yin, X. ,Du, Q. ,Jiang, X. ,Yang, D. ,Lu, W. ,Yang, F. ,Wang, S. ,Wang, S. , Development of an HPLC fingerprint for quality control and species differentiation of Uncaria rhynchophylla (Miq.) ex Havil. Acta Chromatographica Vol. 24, no. 4/2012
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