Development of gradient HPLC-DAD method for assay of ternary mixture containing amebicide and analgesic drugs

Czasopismo : Acta Chromatographica
Tytuł artykułu : Development of gradient HPLC-DAD method for assay of ternary mixture containing amebicide and analgesic drugs

Autorzy :
Komsta, Ł.
Medical University of Lublin Chair and Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland, lukasz.komsta@am.lublin.pl,
Gowin, E.
Medical University of Lublin Chair and Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland,
Djordjević Filijović, N.
Hemofarm AD Vrsac Serbia,
Pavlović, A.
Hemofarm AD Vrsac Serbia,
Nikolić, K.
University of Belgrade, Faculty of Pharmacy Department of Pharmaceutical Chemistry Vojvode Stepe 450 PO Box 146 11000 Belgrade Serbia,
Agbaba, D.
University of Belgrade, Faculty of Pharmacy Department of Pharmaceutical Chemistry Vojvode Stepe 450 PO Box 146 11000 Belgrade Serbia, danica@pharmacy.bg.ac.rs,
Sawant, S.
Sinhgad Technical Education Society’s Smt. Kashibai Navale College of Pharmacy, Kondhwa (Bk.) Pune 411 048 Maharashtra India, sawant99@rediffmail.com,
Barge, V.
PDEA’s Seth Govind Raghunath Sable College of Pharmacy Saswad, Tal-Purandar, Dist-Pune 412301 Maharashtra India,
Tiwari, R.
School of Pharmacy and Technology Department of Pharmaceutical Chemistry, SVKM’s NMIMS Management Shirpur Dist. Dhule 425405 Maharashtra India, ravisun4@rediffmail.com,
Bonde, C.
School of Pharmacy and Technology Department of Pharmaceutical Chemistry, SVKM’s NMIMS Management Shirpur Dist. Dhule 425405 Maharashtra India,
Bothara, K.
Sinhgad Institute of Pharmacy Department of Pharmaceutical Chemistry Narhe Road Pune 410041 Maharashtra India,
Nawaz, M.
United Arab Emirates University Department of Chemistry Al-Ain 17551 UAE, nawwaz@gmail.com,
Arayne, M. S.
University of Karachi Department of Chemistry Karachi 75270 Pakistan,
Sultana, N.
University of Karachi Research Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, Faculty of Pharmacy Karachi 75270 Pakistan,
Haider, A.
University of Karachi Department of Chemistry Karachi 75270 Pakistan,
Hisaindee, S.
United Arab Emirates University Department of Chemistry Al-Ain 17551 UAE,
Youssef, R. M.
University of Alexandria Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry El-Messalah, Alexandria 21521 Egypt, rmmy1973@yahoo.com,
Abstrakty : Validated gradient high-performance liquid chromatography-diode array detection (HPLC-DAD) method has been developed for simultaneous determination of diloxanide furoate (DIL), mebeverine hydrochloride (MBV), and metronidazole (MET) in their pharmaceutical preparation. This method was based on a reversed-phase HPLC separation of the cited drugs on a C-18 column (150 mm × 4.6 mm, 5 μm) with a gradient elution system of 0.05 M phosphate buffer with 1% (v/v) of triethylamine adjusted to pH 3.0-methanol as the mobile phase at the flow rate of 1.0 mL min-1. The separation was carried out at ambient temperature. Quantitation was achieved with DAD detection at 260 nm for DIL and MEB, and at 320 for MET based on peak area with linear calibration curves at concentration ranges 4–30, 1–20, and 1–30 μg mL-1for DIL, MEB, and MET, respectively. The proposed chromatographic method was successfully applied to the determination of the investigated drugs in pharmaceutical preparation. It was validated in compliance with International Conference on Harmonization (ICH) guidelines, in terms of linearity, accuracy, precision, robustness, limits of detection, and quantitation and other aspects of analytical validation.

Słowa kluczowe : HPLC-DAD, diloxanide furoate, mebeverine hydrochloride, metronidazole, gradient elution,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2014
Numer : Vol. 26, no. 1
Strony : 67 – 80
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DOI :
Cytuj : Komsta, Ł. ,Gowin, E. ,Djordjević Filijović, N. ,Pavlović, A. ,Nikolić, K. ,Agbaba, D. ,Sawant, S. ,Barge, V. ,Tiwari, R. ,Bonde, C. ,Bothara, K. ,Nawaz, M. ,Arayne, M. S. ,Sultana, N. ,Haider, A. ,Hisaindee, S. ,Youssef, R. M. , Development of gradient HPLC-DAD method for assay of ternary mixture containing amebicide and analgesic drugs. Acta Chromatographica Vol. 26, no. 1/2014
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