Journal : Acta Chromatographica
Article : Effect of chromatographic conditions on separation and system efficiency for HPLC of selected alkaloids on amide C16 stationary phases

Authors :
Sherma, J.
Department of Chemistry, Lafayette College, Easton, PA 18042, USA,
Vrchotová, N.
Institute of Systems Biology and Ecology AS CR, Branišovská 31, 370 05 České Budějovice, Czech Republic,
Sivakumar, T.
Department of Pharmacy, Faculty of Engineering and Technology, Annamalai University, Annamalainagar, Tamil Nadu-608 002, India,
Petruczynik, A.
Department of Inorganic Chemistry, Medical University, Staszica 6, 20-081 Lublin, Poland,
Abstract : Retention data for 29 alkaloids were determined on an amide embedded RP silica column with different aqueous mobile phases – mixtures of acetonitrile with water, mixtures of acetonitrile with aqueous buffers of pH 3 or pH 7.8, and mixtures of acetonitrile with aqueous buffers containing ionpair reagents or silanol blockers. Improved peak symmetry and separation selectivity for basic solutes were observed when ion-pair reagents or silanol blockers were used as mobile phase additives. The best separation selectivity and most symmetric peaks for the alkaloids were obtained by use of mobile phases containing diethylamine. The effect of diethylamine concentration on retention, peak symmetry, and theoretical plate number was investigated.

Keywords :
Publishing house : University of Silesia in Katowice
Publication date : 2007
Number : No. 19
Page : 48 – 60

: 1 L.A. Ciolino, J.A. Turner, H.A. McCauley, A.W. Smallwood, and T.Y. Yi, J. Chromatogr., 852, 451 (1999)
2 T. Mroczek, K. Głowniak, and A. Właszczyk, J. Chromatogr., 949, 249 (2002)
3 K. Putzbach, C.A. Rimmer, K.E. Sharpless, and L.C. Sander, J. Chromatogr., 1156, 304 (2007)
4 R.J.M. Vervoort, A.J. Debets, H.A. Classens, C.A. Cramers, and G.J. de Jong, J. Chromatogr., 897, 1 (2000)
5 T. Czajkowska, I. Hrabovsky, B. Buszewski, R.K. Gilpin, and M. Jaroniec, J. Chromatogr., 691, 217 (1995)
6 N.S. Wilson, J. Gilroy, J.W. Dolan, and L.R. Snyder, J. Chromatogr., 1026, 91 (2004)
7 P. Kasturi, B. Buszewski, M. Jaroniec, and R.K. Gilpin, J. Chromatogr., 659, 261 (1994)
8 A. Jakab, M. Prodan, and E. Forgacs, J. Pharm. Biomed. Anal., 27, 913 (2002)
9 F. Gritti and G. Guiochon, J. Chromatogr., 1103, 69 (2006)
10 X. Liu, A. Bordunov, M. Tracy, R. Slingsby, N. Avdalovic, and C. Pohl, J. Chromatogr., 1119, 120 (2006)
11 T. Czajkowska and M. Jaroniec, J. Chromatogr., 762, 147 (1997)
12 J. Layne, J. Chromatogr., 957, 149 (2002)
13 D.V. McCalley, J. Chromatogr., 844, 23 (1999)
14 R.J.M. Vervoort, F.A. Maris, and H. Hindriks, J. Chromatogr., 623, 207 (1992)
15 M.R. Euerby and P. Petersson, J. Chromatogr., 1088, 1 (2005)
Qute : Sherma, J. ,Vrchotová, N. ,Sivakumar, T. ,Petruczynik, A. ,Petruczynik, A. , Effect of chromatographic conditions on separation and system efficiency for HPLC of selected alkaloids on amide C16 stationary phases. Acta Chromatographica No. 19/2007