Journal : Acta Chromatographica
Article : Effect of sample-preparation methods on the HPLC quantitation of some phenolic acids in plant materials

Authors :
Sherma, J.
Department of Chemistry, Lafayette College, Easton, PA 18042, USA,
Vrchotová, N.
Institute of Systems Biology and Ecology AS CR, Branišovská 31, 370 05 České Budějovice, Czech Republic,
Sivakumar, T.
Department of Pharmacy, Faculty of Engineering and Technology, Annamalai University, Annamalainagar, Tamil Nadu-608 002, India,
Petruczynik, A.
Department of Inorganic Chemistry, Medical University, Staszica 6, 20-081 Lublin, Poland,
Benkő, B.
PhD School of Semmelweis University, 1085 Budapest, Üllői út 26, Hungary, Division of Pharmacology and Drug Safety, Richter Gedeon Rt., Gyömrői út 21, 1475 Budapest, P.O. Box 27, Hungary, Department of Chemistry and Biochemistry, University of Pécs, Péc,
Mori, M.
Department of Chemistry, Faculty of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu, 376-8515, Japan,
Solangi, A. R.
National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan,
Idris, A. M.
Department of Chemistry, College of Science, King Faisal University, Hofuf 31982, Saudi Arabia,
Milán-Segovia, R.
Laboratorio de Farmacia, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Ave. Manuel Nava No. 6, 78290 San Luis Potosí, S.L.P., Mexico,
Qutab, S. S.
Schazoo Laboratories (Pvt) Ltd, 45 G.T. Road, Lahore-54000, Pakistan,
Agil, M.
Faculty of Pharmacy, Hamdard University New Delhi, 110 062, India,
Dhaneshwar, S. S.
Department of Pharmaceutical Chemistry, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, Pune-411 038, India,
Gannu, R.
Centre for Biopharmaceutics and Pharmacokinetics, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Andhra Pradesh, India,
Popović, G.
Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, P.O. Box 146, 11000 Belgrade, Serbia,
Mhaske, D. V.
Department of Quality Assurance Techniques and Pharmaceutical Chemistry, Bharati Vidyapeeth University, Poona College of Pharmacy, Centre for Advanced Pharmaceutical Research, Erandwane, Pune 411038, Maharashtra State, India,
Mohammad, A.
Analytical Research Laboratory, Department of Applied Chemistry, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh 202002, India,
Jain, G. K.
Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi, 110062, India,
Dembitsky, V. M.
Department of Medicinal Chemistry and Natural Products, School of Pharmacy, P.O. Box 12065, The Hebrew University of Jerusalem, Jerusalem 91120, Israel,
Qiao, C. F.
Chinese Medicine Laboratory, Hong Kong Jockey Club Institute of Chinese Medicine, Hong Kong, China,
Waksmundzka-Hajnos, M.
Department of Inorganic Chemistry, Medical University of Lublin, Staszica 6, 20-081 Lublin, Poland,
Abstract : The extraction yield of phenolic acids from Sambucus nigra L. inflorescence and Polygonum aviculare foliage has been determined for different methods of liquid–solid extraction – Soxhlet extraction, ultrasonicfication (USAE), microwave-assisted extraction (MASE), and accelerated solvent extraction (ASE). Methanol was used as extractant. The crude extracts were evaporated to dryness and prepared for fractionation of the phenolic acids by liquid–liquid extraction (LLE) or solid-phase extraction (SPE). Samples containing the phenolic acid fraction were analysed by RP-HPLC. Quantitative analysis was performed by the external standard method and use of a calibration plot for each standard. It was found that yield depends primarily on the plant material from which the phenolic acid fraction was extracted. For Sambucus nigra L. inflorescence the most effective was exhaustive extraction in a Soxhlet apparatus. For Polygonum aviculare foliage the most effective methods were MASE and USAE. Both LLE and SPE gave similar recoveries and repeatability.

Keywords :
Publishing house : University of Silesia in Katowice
Publication date : 2007
Number : No. 19
Page : 227 – 237

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Qute : Sherma, J. ,Vrchotová, N. ,Sivakumar, T. ,Petruczynik, A. ,Benkő, B. ,Mori, M. ,Solangi, A. R. ,Idris, A. M. ,Milán-Segovia, R. ,Qutab, S. S. ,Agil, M. ,Dhaneshwar, S. S. ,Gannu, R. ,Popović, G. ,Mhaske, D. V. ,Mohammad, A. ,Jain, G. K. ,Dembitsky, V. M. ,Qiao, C. F. ,Waksmundzka-Hajnos, M. ,Waksmundzka-Hajnos, M. , Effect of sample-preparation methods on the HPLC quantitation of some phenolic acids in plant materials. Acta Chromatographica No. 19/2007