Journal : Acta Chromatographica
Article : Efficient method for extraction and isolation of shionone from Aster tataricus L. f. by supercritical fluid extraction and high-speed counter-current chromatography

Authors :
Xu, L. N.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Gai, F. Y.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Mu, G. F.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Gao, Y.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Liu, C. J.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Luan, F.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Mori, M.
Gunma University Department of Chemistry and Chemical Biology, Graduate School of Engineering 1-5-1 Tenjin-cho Kiryu 376-8515 Japan,
Ikeda, T.
Gunma University Department of Chemistry and Chemical Biology, Graduate School of Engineering 1-5-1 Tenjin-cho Kiryu 376-8515 Japan,
Itabashi, H.
Gunma University Department of Chemistry and Chemical Biology, Graduate School of Engineering 1-5-1 Tenjin-cho Kiryu 376-8515 Japan,
Soomro, R.
University of Sindh National Center of Excellence in Analytical Chemistry Jamshoro Pakistan,
Memon, S. Q.
University of Sindh Institute of Advance Research in Chemical Sciences Jamshoro Pakistan,
Ahmed, M. J.
University of Chittagong Laboratory of Analytical Chemistry, Department of Chemistry Chittagong 4331 Bangladesh,
Memon, N.
University of Sindh National Center of Excellence in Analytical Chemistry Jamshoro Pakistan, najmamemon@gmail.com,
Mallah, A.
University of Sindh M.A. Kazi Institute of Chemistry Jamshoro Pakistan,
Ravi, P. R.
BITS-Pilani Hyderabad Campus Pharmacy Department Ranga Reddy (Dist.) Jawaharnagar Andhra Pradesh India, rahulvats1983@gmail.com,
Aditya, N.
BITS-Pilani Hyderabad Campus Pharmacy Department Ranga Reddy (Dist.) Jawaharnagar Andhra Pradesh India, adityamurthy1212@gmail.com,
Vats, R.
BITS-Pilani Hyderabad Campus Pharmacy Department Ranga Reddy (Dist.) Jawaharnagar Andhra Pradesh India, rpunnarao@gmail.com,
Navaneethan, G.
Sona College of Technology (Anna University) Department of Sciences Salem 636 005 India,
Karunakaran, K.
Sona College of Technology (Anna University) Department of Sciences Salem 636 005 India, drkk@sonatech.ac.in,
Elango, K. P.
Gandhigram Rural Institute (Deemed University) Department of Chemistry Gandhigram 624 302 India,
Prichodko, A.
Vilnius University Department of Analytical and Environmental Chemistry Naugarduko 24 LT-03225 Vilnius Lithuania,
Janenaite, E.
Vilnius University Department of Analytical and Environmental Chemistry Naugarduko 24 LT-03225 Vilnius Lithuania,
Smitiene, V.
Vilnius University Department of Analytical and Environmental Chemistry Naugarduko 24 LT-03225 Vilnius Lithuania,
Vickackaite, V.
Vilnius University Department of Analytical and Environmental Chemistry Naugarduko 24 LT-03225 Vilnius Lithuania, vida.vickackaite@chf.vu.lt,
Akhtar, N.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India,
Faiyazuddin, M.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India, md.faiyazuddin@yahoo.co.in,
Mustafa, G.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India,
Sultana, Y.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India,
Baboota, S.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India,
Ali, J.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India,
Wang, D.
Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China,
Bai, A.
inan Municipal Center For Disease Control and Prevention Jinan 250001 P. R. China,
Lin, X.
Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China,
Fang, L.
Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China,
Shu, X.
Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China,
Shi, X.
Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China,
Sun, Q.
Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China,
Wang, X.
Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China, wxjn1998@126.com,
Abstract : Supercritical fluid extraction (SFE) was used to extract shionone from Aster tataricus L. f. The effect of various parameters, i.e., temperature, pressure and sample particle size on yield was investigated with an analytical-scale SFE system to find the optimal conditions. The process was then scaled up by 50 times with a preparative SFE system under the optimized conditions of temperature 40 °C, pressure 30 MPa, and a sample particle size of 40–60 mesh. Then preparative high-speed counter-current chromatography was successfully used for isolation and purification of shionone from the SFE extract with a two-phase solvent system composed of n-hexane-methanol (2:1, volume ratio). The separation produced a total of 75 mg of shionone from 500 mg of the crude extract in one step separation with the purity of 98.7%, respectively, as determined by high-performance liquid chromatography (HPLC) and 92% recovery. The structure of shionone was identified by electrospray ionization-mass spectrometry (ESI-MS), hydrogen-1 nuclear magnetic resonance (1H-NMR), and carbon-13 nuclear magnetic resonance (13C-NMR).

Keywords : supercritical fluid extraction, high-speed counter-current chromatography, Shionone, Aster tataricus L. f.,
Publishing house : University of Silesia in Katowice
Publication date : 2012
Number : Vol. 24, no. 4
Page : 615 – 625

Bibliography
: 1 T.B. Ng, F. Liu, Y. Lu, C.H.K. Cheng, and Z. Wang. Comp. Biochem. Physiol. C, 136, 109–115 (2003)
2 X. Tang, X. Liu, Y. Tang, Y. Liu, and K. Xu, JTCVM, 1, 16–18 (2006)
3 Y. Lu, Y. Dai, Z. Wang, and L. Wang, Chin. Tradit. Herb. Drugs, 30, 360–362 (1999)
4 Editorial Committee of the Pharmacopoeia of People’s Republic of China, The Pharmacopoeia of People’s Republic of China (Part I), Beijing, Chemical Industry Press, P239 (1999)
5 G. Wang, T. Wu, P. Lin, G. Chou, and Z. Wang, Chin. Tradit. Herb. Drugs, 34, 875–876 (2003)
6 J. Jin, C. Zhang, and M. Zhang, Mod. Chin. Med., 10, 20–22 (2008)
7 T. Akihisa, Y. Kimura, K. Koike, T. Tai, K. Yasukawa, K. Arai, Y. Suzuki, and T. Nikaido, Chem. Pharm. Bull., 46, 1824–1826 (1998)
8 Y. Geng, J. Liu, R. Lv, J. Yuan, Y. Lin, and X. Wang, Sep. Purif. Technol., 57, 237–241 (2007)
9 X. Wang, Y. Wang, J. Yuan, Q. Sun, J. Liu, and C. Zheng, J. Chromatogr. A, 1055, 135–140 (2004)
10 D. Gutzeit, V. Wray, P. Winterhalter, and G. Jerz, Chromatographia, 65, 1–7 (2007)
11 P. Dong, C. Xue, and Q. Du, Acta Chromatographica, 20, 269–276 (2008)
12 Q. Du, L. Zhang, H. Cui, K. Wang, L. Xiu, and L. Sheng, Acta Chromatographica, 20, 489–496 (2008)
13 R. Liu, A. Li, A. Sun, J. Cui, and L. Kong, J. Chromatogr. A, 1064, 53–57 (2005)
14 Ito Y, J. Chromatogr. A, 1065, 145–168 (2005)
15 H. Li, K. Fan, and F. Chen, J. Sep. Sci., 29, 699–703 (2006)
16 R. Roscher, and P. Winterhalter, J. Agric. Food Chem., 41, 1452–1457 (1993)
DOI :
Qute : Xu, L. N. ,Gai, F. Y. ,Mu, G. F. ,Gao, Y. ,Liu, C. J. ,Luan, F. ,Mori, M. ,Ikeda, T. ,Itabashi, H. ,Soomro, R. ,Memon, S. Q. ,Ahmed, M. J. ,Memon, N. ,Mallah, A. ,Ravi, P. R. ,Aditya, N. ,Vats, R. ,Navaneethan, G. ,Karunakaran, K. ,Elango, K. P. ,Prichodko, A. ,Janenaite, E. ,Smitiene, V. ,Vickackaite, V. ,Akhtar, N. ,Faiyazuddin, M. ,Mustafa, G. ,Sultana, Y. ,Baboota, S. ,Ali, J. ,Wang, D. ,Bai, A. ,Lin, X. ,Fang, L. ,Shu, X. ,Shi, X. ,Sun, Q. ,Wang, X. ,Wang, X. , Efficient method for extraction and isolation of shionone from Aster tataricus L. f. by supercritical fluid extraction and high-speed counter-current chromatography. Acta Chromatographica Vol. 24, no. 4/2012
facebook