Journal : Acta Chromatographica
Article : Evaluation of HPLC and spectrophotometric methods for analysis of bioactive constituent berberine in stem extracts of Coscinium fenestratum

Authors :
Mansoor, S.
Georgia Institute of Technology School of Chemical and Biomolecular Engineering Atlanta GA 30332-0100 USA,
Tas, C.
Georgia Institute of Technology School of Chemical and Biomolecular Engineering Atlanta GA 30332-0100 USA, ctas@gata.edu.tr,
Patel, D. S.
Pramukh Swami Science and H.D. Patel Arts College Chemistry Department Sarva Vidyalaya Campus Kadi 382 715 Gujarat India,
Sharma, N.
BA Research India Ltd. Bio-Analytical Laboratory Bodakdev, Ahmedabad 380054 Gujarat India,
Patel, M. C.
Pramukh Swami Science and H.D. Patel Arts College Chemistry Department Sarva Vidyalaya Campus Kadi 382 715 Gujarat India,
Patel, B. N.
BA Research India Ltd. Bio-Analytical Laboratory Bodakdev, Ahmedabad 380054 Gujarat India, bhavinpatel27@rediffmail.com,
Shrivastav, P. S.
Gujarat University Department of Chemistry, School of Sciences Navrangpura, Ahmedabad 380009 Gujarat India, pranav_shrivastav@yahoo.com,
Sanyal, M.
St. Xavier’s College Department of Chemistry Navrangpura, Ahmedabad 380009 Gujarat India,
Wang, G. Y.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P. R. China,
Shi, Y. P.
Lanzhou University of Technology School of Petroleum and Chemical Engineering Lanzhou 730050 P. R. China, shiyp@licp.cas.cn,
Akowuah, G. A.
UCSI University Faculty of Applied Sciences No. 1, Jalan Menara Gading, UCSI Heights 56000 Kuala Lumpur Malaysia, wuahmy@yahoo.com,
Okechukwu, P. N.
UCSI University Faculty of Pharmaceutical Sciences No. 1, Jalan Menara Gading, UCSI Heights 56000 Kuala Lumpur Malaysia,
Chiam, N. C.
UCSI University Faculty of Pharmaceutical Sciences No. 1, Jalan Menara Gading, UCSI Heights 56000 Kuala Lumpur Malaysia,
Abstract : High-performance liquid chromatography (HPLC) and ultraviolet (UV) spectrophotometric methods were developed for analysis of berberine, the principal bioactive constituent, in Coscinium fenestratum extracts. The HPLC and UV spectrophotometric determinations were performed at 226, 270, and 340 nm. The methods were validated by assessing linearity, sensitivity, precision, and accuracy. HPLC detection was found to be more effective than spectrophotometric determinations. The content of berberine determined by HPLC method at detection wavelengths of 270 and 340 nm was found to be higher as compared to the value obtained at wavelength of 226 nm. The content of berberine determined by HPLC ranged from 0.98 to 0.99 (%w/w) in the methanol extract, 0.85 to 0.88 (%w/w) in the methanol-water (1:1) extract, and 0.24 to 0.25 in the water extract.

Keywords : berberine, Coscinium fenestratum, extracts, HPLC, spectrophotometric,
Publishing house : University of Silesia in Katowice
Publication date : 2014
Number : Vol. 26, no. 2
Page : 243 – 254

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DOI :
Qute : Mansoor, S. ,Tas, C. ,Patel, D. S. ,Sharma, N. ,Patel, M. C. ,Patel, B. N. ,Shrivastav, P. S. ,Sanyal, M. ,Wang, G. Y. ,Shi, Y. P. ,Akowuah, G. A. ,Okechukwu, P. N. ,Chiam, N. C. ,Chiam, N. C. , Evaluation of HPLC and spectrophotometric methods for analysis of bioactive constituent berberine in stem extracts of Coscinium fenestratum. Acta Chromatographica Vol. 26, no. 2/2014
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