Journal : Acta Chromatographica
Article : Evaluation of a CGC-FID method for qualitative and quantitative analysis of azole antifungal drugs

Authors :
Dejaegher, B.
Vrije Universiteit Brussel — VUB Analytical Chemistry and Pharmaceutical Technology (FABI), Pharmaceutical Institute Laarbeeklaan 103 1090 Brussels Belgium,
Vander Heyden, Y.
Vrije Universiteit Brussel — VUB Analytical Chemistry and Pharmaceutical Technology (FABI), Pharmaceutical Institute Laarbeeklaan 103 1090 Brussels Belgium, yvanvdh@vub.ac.be,
Durón, R. R.
Universidad Autónoma de Nuevo León Departamento de Química Analítica, Facultad de Medicina P.O. Box 2316 Sucursal Tecnológico 64841 Monterrey Nuevo León México,
Almaguer, L. C.
Universidad Autónoma de Nuevo León Departamento de Química Analítica, Facultad de Medicina P.O. Box 2316 Sucursal Tecnológico 64841 Monterrey Nuevo León México,
Garza-Juárez, A.
Universidad Autónoma de Nuevo León Departamento de Química Analítica, Facultad de Medicina P.O. Box 2316 Sucursal Tecnológico 64841 Monterrey Nuevo León México,
Salazar Cavazos, M. L.
Universidad Autónoma de Nuevo León Departamento de Química Analítica, Facultad de Medicina P.O. Box 2316 Sucursal Tecnológico 64841 Monterrey Nuevo León México,
Waksman de Torres, N.
Universidad Autónoma de Nuevo León Departamento de Química Analítica, Facultad de Medicina P.O. Box 2316 Sucursal Tecnológico 64841 Monterrey Nuevo León México, nwaksman@fm.uanl.mx,
Garza-Juárez, A.
UANL, México Department of Analytical Chemistry, Facultad de Medicina Maderoy Aguirre Pequeño Col. Mitras Centro C.P. 64460 Mty, N.L. México,
Waksman de Torres, N.
UANL, México Department of Analytical Chemistry, Facultad de Medicina Maderoy Aguirre Pequeño Col. Mitras Centro C.P. 64460 Mty, N.L. México,
Salazar Cavazos, M. L.
UANL, México Department of Analytical Chemistry, Facultad de Medicina Maderoy Aguirre Pequeño Col. Mitras Centro C.P. 64460 Mty, N.L. México, masalaz52@yahoo.com,
Durón, R. R.
UANL, México Department of Analytical Chemistry, Facultad de Medicina Maderoy Aguirre Pequeño Col. Mitras Centro C.P. 64460 Mty, N.L. México,
Briciu, R. D.
Babeş-Bolyai University Faculty of Chemistry and Chemical Engineering Arany Janos Str. No 11 RO-400028 Cluj Napoca Romania,
Kot-Wasik, A.
Gdańsk University of Technology Chemical Faculty Narutowicza Str. No 11/12 80-952 Gdańsk Poland,
Namieśnik, J.
Gdańsk University of Technology Chemical Faculty Narutowicza Str. No 11/12 80-952 Gdańsk Poland,
Sârbu, C.
Babeş-Bolyai University Faculty of Chemistry and Chemical Engineering Arany Janos Str. No 11 RO-400028 Cluj Napoca Romania, costelsrb@yahoo.co.uk,
Sławik, T.
Medical University of Lublin Department of Medicinal Chemistry, Pharmaceutical Faculty Jaczewskiego 4 20-090 Lublin Poland,
Skibiński, R.
Medical University of Lublin Department of Medicinal Chemistry, Pharmaceutical Faculty Jaczewskiego 4 20-090 Lublin Poland, robert.skibinski@am.lublin.pl,
Paw, B.
Medical University of Lublin Department of Medicinal Chemistry, Pharmaceutical Faculty Jaczewskiego 4 20-090 Lublin Poland,
Działo, G.
Medical University of Lublin Department of Medicinal Chemistry, Pharmaceutical Faculty Jaczewskiego 4 20-090 Lublin Poland,
Sajewicz, M.
Silesian University Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Matlengiewicz, M.
Silesian University Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Kronenbach, D.
Silesian University Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Gontarska, M.
Silesian University Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland,
Kowalska, T.
Silesian University Institute of Chemistry 9 Szkolna Street 40-006 Katowice Poland, kowalska@us.edu.pl,
Ekiert, R. J.
Jagiellonian University Department of Inorganic and Analytical Chemistry, Collegium Medicum 9 Medyczna St 30-688 Kraków Poland,
Krzek, J.
Jagiellonian University Department of Inorganic and Analytical Chemistry, Collegium Medicum 9 Medyczna St 30-688 Kraków Poland, jankrzek@cm-uj.krakow.pl,
Czekaj, J. S.
Jagiellonian University Department of Inorganic and Analytical Chemistry, Collegium Medicum 9 Medyczna St 30-688 Kraków Poland,
Hubicka, U.
Jagiellonian University Department of Inorganic and Analytical Chemistry, Collegium Medicum 9 Medyczna St 30-688 Kraków Poland,
Abstract : This paper presents a new method for identification and quantitative analysis of six azole antifungal drugs — bifonazole, clotrimazole, econazole, fluconazole, ketoconazole, and miconazole — by capillary gas chromatography (CGC) combined with flame ionization detection (FID). The chromatographic separation conditions were established and the method was validated for precision (RSD = 1.49–3.55%), recovery (98.6–101.2%), and linearity within the range under investigation (∼1.0–33.3 ng). The results obtained show the newly developed procedure is suitable for qualitative and quantitative pharmaceutical analysis of the six azoles.

Keywords : azole antifungal drugs, pharmaceutical analysis, capillary gas chromatography, flame-ionization detection,
Publishing house : University of Silesia in Katowice
Publication date : 2009
Number : Vol. 21, no. 2
Page : 273 – 282

Bibliography
: 1 J.A. Maertens, Clin. Microbiol. Infect., 10 (Suppl. 1), 1 (2004)
2 D.J. Sheehan, C.A. Hitchcock, and C.M. Sibley, Clin. Microbiol. Rev., 12 , 40 (1999)
3 W.E. Dismukes, Clin. Infect. Dis., 30 , 653 (2000)
4 P.Y. Khashaba, S.R. El-Shabouri, K.M. Emara, and A.M. Mohamed, J. Pharm. Biomed. Anal., 22 , 363 (2000)
5 O. Lortholary, M. Nicolas, S. Sorea, L. Improvisi, O. Ronin, O. Petitjean, B. Dupont, M. Tod, and F. Dromer, J. Antimicrob. Chemother., 43 , 817 (1999)
6 T. Peng, Q. Cheng, and C.F. Yang, Fresenius J. Anal. Chem., 370 , 1082 (2001)
7 E.M. Abdel-Moety, F.I. Khattab, K.M. Kelani, and A.M. AbouAl-Alamein, Il Farmaco, 57 , 931 (2003)
8 R.J. Ekiert, J. Krzek, and W. Rzeszutko, Chromatographia, 67 , 995 (2008)
9 E. Kublin and T. Kaniewska, Chem. Anal. (Warsaw), 41 , 19 (1996)
10 M. Peschka, P.H. Roberts, and T.P. Knepper, Anal. Bioanal. Chem., 389 , 959 (2007)
11 J.J.P. Heykants, R.J.H. Woestenborghs, M.P.J.M. Bisschop, and J.M.W.M. Merkus, Eur. J. Clin. Pharmacol., 23 , 331 (1982)
12 S.C. Szathmary and I. Luhmann, J. Chromatogr., 425 , 193 (1988)
13 T.K. Daneshmend, J. Antimicrob. Chemother., 18 , 507 (1986)
14 S. Khundker, J.R. Dean, and P. Jones, J. Pharm. Biomed. Anal., 13 , 1441 (1995)
15 D.M. Lima, G.P. Nunes Jr, P.H. Ferri, and S.C. Santos, Rev. Bras. Cienc. Farm., 41 , 223 (2005)
16 D. Debruyne, J.P. Ryckelynck, M.C. Bigot, and M. Moulin, J. Pharm. Sci., 77 , 534 (1988)
17 S.C. Harris, J.E. Wallace, G. Foulds, and M.G. Rinaldi, Antimicrob. Agents Chemother., 33 , 714 1989)
18 A.B. Rege, J.Y. Walker-Cador, R.A. Clark, J.J.L. Lertora, N.E. Hyslop Jr, and W.J. George, Antimicrob. Agents Chemother., 36 , 647 (1992)
19 P.R. Wood and M.H. Tarbit, J. Chromatogr., 383 , 179 (1986)
20 ICH Harmonised Tripartite Guideline, Validation of Analytical Procedures: Text and Methodology. Q2(R1), 2005
21 European Pharmacopoeia 6.0, Council of Europe, Strasbourg, 2008, pp. 1098, 1333, 1493, 1872, 1900, and 2043
22 United States Pharmacopeia 31, The United States Pharmacopeial Convention, Rockville, 2008, pp. 1825, 1826, 1827, 1828, 1829, 2037, 2171, 2488, 2489, 2711, 2712, 2713
DOI :
Qute : Dejaegher, B. ,Vander Heyden, Y. ,Durón, R. R. ,Almaguer, L. C. ,Garza-Juárez, A. ,Salazar Cavazos, M. L. ,Waksman de Torres, N. ,Garza-Juárez, A. ,Waksman de Torres, N. ,Salazar Cavazos, M. L. ,Durón, R. R. ,Briciu, R. D. ,Kot-Wasik, A. ,Namieśnik, J. ,Sârbu, C. ,Sławik, T. ,Skibiński, R. ,Paw, B. ,Działo, G. ,Sajewicz, M. ,Matlengiewicz, M. ,Kronenbach, D. ,Gontarska, M. ,Kowalska, T. ,Ekiert, R. J. ,Krzek, J. ,Czekaj, J. S. ,Hubicka, U. ,Hubicka, U. , Evaluation of a CGC-FID method for qualitative and quantitative analysis of azole antifungal drugs. Acta Chromatographica Vol. 21, no. 2/2009
facebook