Journal : Acta Chromatographica
Article : Evaluation of separation parameters for selected organophosphorus fungicides of forensic importance by RP-HPTLC

Authors :
Sajewicz, M.
University of Silesia Department of General Chemistry and Chromatography 9 Szkolna Street 40-006 Katowice Poland,
Kowalska, T.
University of Silesia Department of General Chemistry and Chromatography 9 Szkolna Street 40-006 Katowice Poland, teresa.kowalska@us.edu.pl,
Szalka, M.
ICN Polfa Rzeszów S.A. Product Development Department 35-959 Rzeszów Poland,
Kostka, J.
Rzeszów University of Technology Department of Chemical and Process Engineering 35-959 Rzeszów,
Rokaszewski, E.
ICN Polfa Rzeszów S.A. Product Development Department 35-959 Rzeszów Poland,
Kaczmarski, K.
Rzeszów University of Technology Department of Chemical and Process Engineering 35-959 Rzeszów, kkaczmarski@prz.edu.pl,
Belal, F.
University of Mansoura Department of Analytical Chemistry, Faculty of Pharmacy 35516 Mansoura Egypt,
El-Brashy, A. M.
University of Mansoura Department of Analytical Chemistry, Faculty of Pharmacy 35516 Mansoura Egypt,
El-Enany, N.
University of Mansoura Department of Analytical Chemistry, Faculty of Pharmacy 35516 Mansoura Egypt,
Tolba, M. M.
University of Mansoura Department of Analytical Chemistry, Faculty of Pharmacy 35516 Mansoura Egypt,
Cielecka-Piontek, J.
Poznan University of Medical Sciences Department of Pharmaceutical Chemistry, Faculty of Pharmacy Grunwaldzka 6 60-780 Poznań Poland, jpiontek@ump.edu.pl,
Krause, A.
PozLab Contract Research Organization Poznań Poland,
Zalewski, P.
Poznan University of Medical Sciences Department of Pharmaceutical Chemistry, Faculty of Pharmacy Grunwaldzka 6 60-780 Poznań Poland,
Lunzer, A.
Tanaka Laboratory in Hokkaido University W 7, Kita-ku Sapporo 060-8638 Japan,
Jelińska, A.
Poznan University of Medical Sciences Department of Pharmaceutical Chemistry, Faculty of Pharmacy Grunwaldzka 6 60-780 Poznań Poland,
Sarju, N.
Universiti Teknologi Malaysia Department of Industrial Biotechnology, Faculty of Biosciences and Bioengineering 81310 Skudai, Johor Bahru, Johor Malaysia,
Abd Samad, A.
Universiti Teknologi Malaysia Department of Industrial Biotechnology, Faculty of Biosciences and Bioengineering 81310 Skudai, Johor Bahru, Johor Malaysia,
Abd Ghani, M.
Universiti Kebangsaan Malaysia School of Chemical Sciences and Food Technology 43600 UKM Bangi, Selangor Malaysia,
Ahmad, F.
Universiti Teknologi Malaysia Department of Chemistry, Faculty of Science 81310 Skudai, Johor Bahru, Johor Malaysia, farediah@kimia.fs.utm.my,
Yang, G. J.
China Pharmaceutical University School of Pharmacy Nanjing 210009 P. R. China, yanggongjun888@163.com,
Lv, L.
Yangzhou University College of Chemistry and Chemical Engineering Yangzhou 225002 P. R. China,
O’sullivan, C.
Lafayette College Department of Chemistry Easton PA 18042 USA,
Sherma, J.
Lafayette College Department of Chemistry Easton PA 18042 USA, shermaj@lafayette.edu,
Nagaraju, P. M.
Forensic Science Laboratory Madivala, Bangalore 560068 Karnataka India,
Sanganalmath, P. U.
Forensic Science Laboratory Madivala, Bangalore 560068 Karnataka India,
Kemparaju, K.
Mysore University Department of Studies in Biochemistry, Manasa Gangothri Mysore Karnataka India,
Mohan, B. M.
Forensic Science Laboratory Madivala, Bangalore 560068 Karnataka India, praveen_usp@yahoo.com,
Abstract : Studies on the evaluation of separation efficacies are of primary interest to forensic analysts for newly available pesticides. In the present work, a method is described for the separation evaluation of three selected organophosphorus fungicides (OPFs) ditalimfos (D), edifenfos (E), and tolclofos-methyl (TM) by reversed-phase high-performance thin-layer chromatography (RP-HPTLC). The method is based on the separation of three OPFs on silica gel 60 RP-18WF254 plates with varying mobile phase compositions (10:0–6:4) of methanol-water. The relationship between the mobile phase composition and retention parameters (RF and RM) as well as separation parameters (ΔRF, RS, α, RFα) was analyzed. The three OPFs are completely separated from each other with ΔRF ≥ 0.04, except for the pair of compounds E-TM in the mobile phase composition 10:0, 7:3, 6.5:3.5, and 6:4. Under the chromatographic conditions used, TM was adsorbed the strongest, followed by E and D, which is indicated by the higher RM values obtained for TM. Peak resolution (RS) values greater than 1.5 were obtained over the entire range of mobile phase composition, except for the pair of compounds E-TM. The UV apex of maximum absorption was 200 nm for all the three OPFs as measured by multiwave-length scan in the UV range, and higher values of peak area and peak height were obtained at the same wavelength. The present study clearly indicates that the system of RPHPTLC with mobile phase methanol-water in a volume composition 8:2 (υ/υ) provides the optimum conditions for the separation of the three OPFs. However, the complete separation of E from TM was not achieved using this system.

Keywords : densitometry, RP-HPTLC, organophosphorus fungicides, separation parameters,
Publishing house : University of Silesia in Katowice
Publication date : 2012
Number : Vol. 24, no. 2
Page : 253 – 262

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DOI :
Qute : Sajewicz, M. ,Kowalska, T. ,Szalka, M. ,Kostka, J. ,Rokaszewski, E. ,Kaczmarski, K. ,Belal, F. ,El-Brashy, A. M. ,El-Enany, N. ,Tolba, M. M. ,Cielecka-Piontek, J. ,Krause, A. ,Zalewski, P. ,Lunzer, A. ,Jelińska, A. ,Sarju, N. ,Abd Samad, A. ,Abd Ghani, M. ,Ahmad, F. ,Yang, G. J. ,Lv, L. ,O’sullivan, C. ,Sherma, J. ,Nagaraju, P. M. ,Sanganalmath, P. U. ,Kemparaju, K. ,Mohan, B. M. ,Mohan, B. M. , Evaluation of separation parameters for selected organophosphorus fungicides of forensic importance by RP-HPTLC. Acta Chromatographica Vol. 24, no. 2/2012
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