Evaluation of thin-layer chromatography systems for analysis of amino acids in complex mixtures

Czasopismo : Acta Chromatographica
Tytuł artykułu : Evaluation of thin-layer chromatography systems for analysis of amino acids in complex mixtures

Autorzy :
Turło, J.
Medical University of Warsaw Department of Drug Technology 1 Banacha Street 02-097 Warsaw Poland Medical University of Warsaw Faculty of Pharmacy 1 Banacha Street 02-097 Warsaw Poland, jadwiga.turlo@wum.edu.p,
Komsta, Ł.
Medical University of Lublin Chair and Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland Medical University of Lublin Chair and Department of Pharmaceutical Botany, Faculty of Pharmacy Chodźki 1 20-093 Lublin Pola, lukasz.komsta@am.lublin.pl,
Vasta, J. D.
Lafayette College Department of Chemistry Easton PA 18042-1782 USA Lafayette College Department of Biology Easton PA 18042-1782 USA, shermaj@mail.lafayette.edu,
Abstrakty : Different thin-layer chromatography (TLC) systems were evaluated for analysis of 21 biologically important essential and nonessential amino acids in complex mixtures such as biological tissues and fluids. Amino acids were visualized on the layers by derivatization with ninhydrin reagent, and RFvalues were determined by slit-scanning densitometry. The five systems found to be most useful for analysis of amino acids were cellulose and silica gel high-performance TLC (HPTLC) plates developed with either 2-butanol-pyridine-glacial acetic acid-deionized water, 39:34:10:26, or 2-butanol-pyridine-25% ammonia-deionized water, 39:34:10:26, and ion exchange TLC plates developed with citrate buffer, pH 3.3. Using these five systems with ninhydrin detection, identification of all amino acids except for leucine and isoleucine in complex mixtures is possible, and quantification can be achieved if the amino acid to be quantified is well separated from adjacent components of the mixture. Example chromatograms are illustrated for separation and identification of amino acids in a snail tissue sample on a cellulose HPTLC plate.

Słowa kluczowe : amino acids, thin-layer chromatography, TLC, biological samples, Biomphalaria glabrata,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2009
Numer : Vol. 21, no. 1
Strony : 29 – 38
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DOI :
Cytuj : Turło, J. ,Komsta, Ł. ,Vasta, J. D. , Evaluation of thin-layer chromatography systems for analysis of amino acids in complex mixtures. Acta Chromatographica Vol. 21, no. 1/2009
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