Journal : Acta Chromatographica
Article : Facile assay method for norfloxacin and ciprofloxacin by sequential injection chromatography

Authors :
Mansoor, S.
Georgia Institute of Technology School of Chemical and Biomolecular Engineering Atlanta GA 30332-0100 USA,
Tas, C.
Georgia Institute of Technology School of Chemical and Biomolecular Engineering Atlanta GA 30332-0100 USA, ctas@gata.edu.tr,
Patel, D. S.
Pramukh Swami Science and H.D. Patel Arts College Chemistry Department Sarva Vidyalaya Campus Kadi 382 715 Gujarat India,
Sharma, N.
BA Research India Ltd. Bio-Analytical Laboratory Bodakdev, Ahmedabad 380054 Gujarat India,
Patel, M. C.
Pramukh Swami Science and H.D. Patel Arts College Chemistry Department Sarva Vidyalaya Campus Kadi 382 715 Gujarat India,
Patel, B. N.
BA Research India Ltd. Bio-Analytical Laboratory Bodakdev, Ahmedabad 380054 Gujarat India, bhavinpatel27@rediffmail.com,
Shrivastav, P. S.
Gujarat University Department of Chemistry, School of Sciences Navrangpura, Ahmedabad 380009 Gujarat India, pranav_shrivastav@yahoo.com,
Sanyal, M.
St. Xavier’s College Department of Chemistry Navrangpura, Ahmedabad 380009 Gujarat India,
Wang, G. Y.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 P. R. China,
Shi, Y. P.
Lanzhou University of Technology School of Petroleum and Chemical Engineering Lanzhou 730050 P. R. China, shiyp@licp.cas.cn,
Akowuah, G. A.
UCSI University Faculty of Applied Sciences No. 1, Jalan Menara Gading, UCSI Heights 56000 Kuala Lumpur Malaysia, wuahmy@yahoo.com,
Okechukwu, P. N.
UCSI University Faculty of Pharmaceutical Sciences No. 1, Jalan Menara Gading, UCSI Heights 56000 Kuala Lumpur Malaysia,
Chiam, N. C.
UCSI University Faculty of Pharmaceutical Sciences No. 1, Jalan Menara Gading, UCSI Heights 56000 Kuala Lumpur Malaysia,
Raszewski, G.
Institute of Agricultural Medicine Department of Pathophysiology Lublin Poland, grasz@galen.imw.lublin.pl,
Juszczak, M.
Institute of Agricultural Medicine Department of Medical Biology Lublin Poland,
Lemieszek, M. K.
Institute of Agricultural Medicine Department of Medical Biology Lublin Poland,
Matysiak, J.
University of Life Sciences Department of Chemistry Lublin Poland,
Niewiadomy, A.
University of Life Sciences Department of Chemistry Lublin Poland,
Rzeski, W.
Maria Curie-Sklodowska University Department of Virology and Immunology, Institute of Microbiology and Biotechnology Lublin Poland,
Reddy, P. S.
IPDO Dr. Reddy’s Laboratories Ltd. Bachupally, Hyderabad 500072 A.P India, sunilpsr@yahoo.com,
Babu, K. S.
S. K. University Department of Chemistry Anantapur 515055 A.P. India,
Kumar, N.
IPDO Dr. Reddy’s Laboratories Ltd. Bachupally, Hyderabad 500072 A.P India,
Pareek, D.
Rajiv Gandhi Technological University School of Pharmaceutical Sciences Bhopal 462033 Madhya Pradesh India,
Jain, S.
Sagar Institute of Research and Technology Ayodhya bypass, Bhopal 462 041 MP India,
Basniwa, P. K.
Rajiv Gandhi Technological University School of Pharmaceutical Sciences Bhopal 462033 Madhya Pradesh India,
Jain, D.
Rajiv Gandhi Technological University School of Pharmaceutical Sciences Bhopal 462033 Madhya Pradesh India, deepti2515@yahoo.com,
Wróblewski, K.
Medical University of Lublin Department of Inorganic Chemistry 4a Chodźki Str. 20-093 Lublin Poland,
Petruczynik, A.
Medical University of Lublin Department of Inorganic Chemistry 4a Chodźki Str. 20-093 Lublin Poland,
Waksmundzka-Hajnos, M.
Medical University of Lublin Department of Inorganic Chemistry 4a Chodźki Str. 20-093 Lublin Poland, monika.hajnos@umlub.pl,
Jain, P. S.
R. C. Patel Institute of Pharmaceutical Education and Research Karwand Naka, Shirpur, Dist. Dhule 425 405 MS India, pritash79@yahoo.com,
Chaudhari, A. J.
R. C. Patel Institute of Pharmaceutical Education and Research Karwand Naka, Shirpur, Dist. Dhule 425 405 MS India,
Surana, S. J.
R. C. Patel Institute of Pharmaceutical Education and Research Karwand Naka, Shirpur, Dist. Dhule 425 405 MS India,
Elgorashe, R. E. E.
Sudan University of Science and Technology Department of Chemistry, College of Science Khartoum Sudan,
Idris, A. M.
King Khalid University Department of Chemistry, College of Science Abha Saudi Arabia, abubakridris@hotmail.com,
Abdelrahman, M. A.
Sudan University of Science and Technology Department of Chemistry, College of Science Khartoum Sudan,
Saeed, A. E. M.
Sudan University of Science and Technology Department of Chemistry, College of Science Khartoum Sudan,
Abstract : Sequential injection chromatography (SIC) is a simple, inexpensive, portable, reagent-saving, and environmentally benign separation technique. This paper reports the development of a facile assay method for norfloxacin (NF) and ciprofloxacin (CF) in their formulations (tablets and eye drops) using SIC. The analytes were rapidly chromatographed on a short C18 monolithic column (4.6 × 50 mm) at a flow rate of 30 μL s-1. An isocratic mode was adopted. The optimum mobile phase composition was 90% water, 10% acetonitrile, and 0.3% triethylamine at pH 3.0. Miniaturized fiber optic spectrometric devices were coupled to the SIC system for UV detection at 280 nm. Sufficient separation with a resolution of 1.6 and numbers of theoretical plates of more than 1500 was achieved. Additionally, acceptable peak shapes with peak symmetries of 0.89 and 0.92 for NF and CF, respectively, were obtained. The method was validated and found to be reliable (the recovery was 98.0–100.6%) and precise (the RSDs of intra-/inter-day precision were less than 2.0%). The method is also sensitive. The limits of detection were less than 0.27 μg mL-1. The provided method could be considered an industrial scale since the sample frequency was seven samples h-1and the total volume of consumed reagents was 11.5 mL.

Keywords : sequential injection chromatography, norfloxacin, ciprofloxacin, method validation,
Publishing house : University of Silesia in Katowice
Publication date : 2014
Number : Vol. 26, no. 2
Page : 321 – 334

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DOI :
Qute : Mansoor, S. ,Tas, C. ,Patel, D. S. ,Sharma, N. ,Patel, M. C. ,Patel, B. N. ,Shrivastav, P. S. ,Sanyal, M. ,Wang, G. Y. ,Shi, Y. P. ,Akowuah, G. A. ,Okechukwu, P. N. ,Chiam, N. C. ,Raszewski, G. ,Juszczak, M. ,Lemieszek, M. K. ,Matysiak, J. ,Niewiadomy, A. ,Rzeski, W. ,Reddy, P. S. ,Babu, K. S. ,Kumar, N. ,Pareek, D. ,Jain, S. ,Basniwa, P. K. ,Jain, D. ,Wróblewski, K. ,Petruczynik, A. ,Waksmundzka-Hajnos, M. ,Jain, P. S. ,Chaudhari, A. J. ,Surana, S. J. ,Elgorashe, R. E. E. ,Idris, A. M. ,Abdelrahman, M. A. ,Saeed, A. E. M. ,Saeed, A. E. M. , Facile assay method for norfloxacin and ciprofloxacin by sequential injection chromatography. Acta Chromatographica Vol. 26, no. 2/2014
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