Journal : Acta Chromatographica
Article : Fingerprint of ethyl acetate extraction combined with qualitative and quantitative analysis on Patrinia scabra Bunge: Distinguish P. scabra Bunge from its confusable species

Authors :
Gwarda, R. Ł.
Medical University of Lublin Department of Physical Chemistry, Chair of Chemistry 4a Chodźki Street 20-093 Lublin Poland, radoslaw.gwarda@umlub.pl,,
Tomczyszyn, A.
University of Warsaw Faculty of Chemistry 1 Pasteura Street 02-093 Warsaw Poland,
Misicka, A.
University of Warsaw Faculty of Chemistry 1 Pasteura Street 02-093 Warsaw Poland,
Dzido, T. H.
Medical University of Lublin Department of Physical Chemistry, Chair of Chemistry 4a Chodźki Street 20-093 Lublin Poland,
Xue, X. D.
Yunnan Normal University Department of Chemistry Kunming 650092 P.R. China,
Zhang, M.
East China Normal University Department of Chemistry Shanghai 200241 P.R. China,
Xie, S. M.
Yunnan Normal University Department of Chemistry Kunming 650092 P.R. China,
Yuan, L. M.
Yunnan Normal University Department of Chemistry Kunming 650092 P.R. China, yuan_limingpd@yahoo.com.cn,
Matta, M. K.
Jawaharlal Nehru Technological University University College of Pharmaceutical Sciences Hyderabad 500 085 India,
Pilli, N. R.
Jawaharlal Nehru Technological University University College of Pharmaceutical Sciences Hyderabad 500 085 India,
Rao, J. V. L. N. S.
Yalamarty College of Pharmaceutical Sciences Visakhapatnam 530 052 India, jvlnsrao@rediffmail.com,
Abdelhay, M. H.
University of Alexandria Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Elmessalah, Alexandria 21521 Egypt,
Gazy, A. A.
University of Alexandria Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Elmessalah, Alexandria 21521 Egypt,
Shaalan, R. A.
rasha_shaalan@yahoo.com,
Ashour, H. K.
University of Alexandria Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Elmessalah, Alexandria 21521 Egypt,
Krasucka, D.
National Veterinary Research Institute — National Research Institute Department of Veterinary Pharmacy Al. Partyzantów 57 24-100 Pulawy Poland,
Kowalski, C. J.
University of Life Sciences in Lublin Department of Pharmacology, Faculty of Veterinary Medicine Akademicka 12 20-033 Lublin Poland,
Osypiuk, M.
University of Life Sciences in Lublin Department of Pharmacology, Faculty of Veterinary Medicine Akademicka 12 20-033 Lublin Poland, monika.osypiuk@up.lublin.pl,
Opielak, G.
Medical University School of Lublin Human Anatomy Department Jaczewskiego 4 20-090 Lublin Poland,
Mahgoub, H.
King Abdulaziz University Department of Pharmaceutical Chemistry, Faculty of Pharmacy Jeddah Saudi Arabia, hodamahgoub@yahoo.com,
Hanafy, A.
King Abdulaziz University Department of Pharmacology and Toxicology, Faculty of Pharmacy Jeddah Saudi Arabia,
Bamane, F. H.
King Abdulaziz University Department of Biochemistry, Faculty of Medicine Jeddah Saudi Arabia,
Radwan, A. E.
University of Alexandria Department of Biotechnology, Institute of Graduate Studies and Research Alexandria Egypt,
Wen, C.
Wenzhou Medical College Laboratory Animal Centre Wenzhou 325035 China,
Zhang, Q.
Shanghai Institute of Pharmaceutical Industry Shanghai 200437 China,
He, Y.
Wenzhou Medical College Laboratory Animal Centre Wenzhou 325035 China,
Deng, M.
Wenzhou Medical College Laboratory Animal Centre Wenzhou 325035 China,
Wang, X.
Wenzhou Medical College Laboratory Animal Centre Wenzhou 325035 China,
Ma, J.
Wenzhou Medical College Function Experiment Teaching Center Wenzhou 325035 China, jianshema@gmail.com,
Wang, Y.
National Institutes for Food and Drug control (NIFDC) Beijing 100050 P.R. China,
Wang, M. J.
National Institutes for Food and Drug control (NIFDC) Beijing 100050 P.R. China,
Li, J.
National Institutes for Food and Drug control (NIFDC) Beijing 100050 P.R. China,
Yao, S. C.
National Institutes for Food and Drug control (NIFDC) Beijing 100050 P.R. China,
Xue, J.
National Institutes for Food and Drug control (NIFDC) Beijing 100050 P.R. China,
Zou, W. B.
National Institutes for Food and Drug control (NIFDC) Beijing 100050 P.R. China,
Hu, C.
National Institutes for Food and Drug control (NIFDC) Beijing 100050 P.R. China, hucq@nicpbp.org.cn,
Idris, A. M.
King Khalid University Department of Chemistry, College of Science Abha Saudi Arabia, abubakridris@hotmail.com,
Alnajjar, A. O.
King Faisal University Department of Chemistry, College of Science Hofuf Saudi Arabia,
Vichapong, J.
Khon Kaen University Materials Chemistry Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science Khon Kaen 40002 Thailand,
Burakham, R.
Khon Kaen University Materials Chemistry Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science Khon Kaen 40002 Thailand, rodjbu@kku.ac.th,
Srivastava, P.
Agharkar Research Institute Chemistry Group, Animal Science Division G. G. Agarkar Road Pune 411004 India, psri94@yahoo.com,
Raut, H. N.
Agharkar Research Institute Chemistry Group, Animal Science Division G. G. Agarkar Road Pune 411004 India,
Puntambekar, H. M.
Agharkar Research Institute Chemistry Group, Animal Science Division G. G. Agarkar Road Pune 411004 India,
Desai, A. C.
Indian Drug Research Association and Laboratory Drug Analysis 561/B, Shivajinagar Pune 411005 India,
Wang, F.
Central South University Xiangya Hospital Changsha Hunan 410008 China,
Liu, S.
Central South University Xiangya Hospital Changsha Hunan 410008 China, liushao999@hotmail.com,
Luo, M. Y.
Shanghai Medical College of Fudan University Shanghai 200032 China,
Qin, Y.
Jiangsu Shenlong Pharmaceutical Co., Ltd. Yanchen Jiangsu 224200 China,
Lei, P.
Central South University Xiangya Hospital Changsha Hunan 410008 China,
Liu, Y. H.
Central South University Xiangya Hospital Changsha Hunan 410008 China,
Liang, Y. Z.
Central South University College of Chemistry and Chemical Engineering Changsha Hunan 410083 China,
Liu, W.
China Pharmaceutical University Department of Pharmaceutical Analysis 24 Tongjiaxiang Nanjing 210009 China,
Wei, M. L.
China Pharmaceutical University Department of Pharmaceutical Analysis 24 Tongjiaxiang Nanjing 210009 China,
Wu, C. Y.
China Pharmaceutical University Department of Pharmaceutical Analysis 24 Tongjiaxiang Nanjing 210009 China,
Zhu, H. Y.
China Pharmaceutical University Department of Pharmaceutical Analysis 24 Tongjiaxiang Nanjing 210009 China,
Feng, F.
China Pharmaceutical University Department of Natural Medicinal Chemistry 24 Tongjiaxiang Nanjing 210009 China, liuwenyuan8506@163.com,
Xie, N.
Jiangxi Qingfeng Pharmaceutical Ltd. Ganzhou 341000 China,
Abstract : Patrinia scabra Bunge has long been used in clinic as a traditional Chinese medicine for treating leukemia and cancer and regulating host immune response. Despite their wide use in China, no report on system analysis on their chemical constituents is available so far. The current study was designed to profile the fingerprint of ethyl acetate extract of it, and in addition, to characterize the major fingerprint peaks and determine their quantity. Therefore, a detailed gradient high-performance liquid chromatography was described to separate more than 30 compounds with satisfactory resolution in P. scabra Bunge. Based on the chromatograms of 10 batches samples, a typical high-performance liquid chromatographic (HPLC) fingerprint was established with 23 chromatographic peaks being assigned as common fingerprint peaks. Furthermore, a quadrupole time of flight mass spectrometry (Q-TOF/MS) was coupled for the characterization of major compound. As (+)-nortrachelogenin was the most predominant compound in P. scabra Bunge, the quantification on it was also carried out with the method being validated. As a result, (+)-nortrachelogenin was found to be from 1.33 to 2.21 mg g−1 in this plant material. This rapid and effective analytical method could be employed for quality assessment of P. scabra Bunge, as well as pharmaceutical products containing this herbal material.

Keywords : Patrinia scabra Bunge, fingerprint construction, quantitative analysis, method validation, HPLC-Q-TOF/MS,
Publishing house : University of Silesia in Katowice
Publication date : 2015
Number : Vol. 27, no. 1
Page : 177 – 187

Bibliography
: 1 R.H. Liu, W.D. Zhang, Z.B Gu, C. Zhang, J. Su, and X.K. Xu, Nat. Prod. Res., 20, 866–870 (2006)
2 H. Sun, C. Sun, Y. Pan, Chem. Biodiversity, 2, 1351–1357 (2005)
3 J.H. Chen and X.X. Wang, J. Chin. Med. Mater., 31, 1689–1691 (2008)
4 Y. Konishi, T. Kiyota, C. Draghici, J.M. Gao, F. Yeboah, S. Acoca, S. Jarussophon, and E. Purisima, Anal. Chem., 79, 1187–1197 (2007)
5 F.L. Liu, F. Feng, and W.Y. Liu, Pharm. Clin. Res., 18, 356–359 (2010)
6 A. Kouno, I. Yasuda, H. Mizoshiri, T. Tanaka, N. Marubayashi, and D.M. Yang, Phytochemistry, 37, 467–472 (1994)
7 Q. Wu, Q. Yuan, E.H. Liu, L.W. Qi, Z.M. Bi, and P. Li, Biomed. Chromatogr., 24, 808–819 (2010)
8 G.L. Li, Y.A. Liu, J.B. Chang, C.L. Zhang, and J.X. Xie, J. Chin. Mass. Spectrom. Soc., 18, 27–34 (1997)
9 Z.B. Gu, X.J. Chen, G.J. Yang, T.Z. Li, W.Y. Liu, and W.D. Zhang, J. Chin. Med. Mater., 25, 178–180 (2002)
10 B. Vermes, O. Seligmann, and H. Wagner, Phytochemistry, 30, 3087–3089 (1991)
11 G.J. Yang, Z.B. Gu, W.Y. Liu, Y. Qiu, T.Z. Li, and W.D. Zhang, J. Asian Nat. Prod. Res., 6, 277–280 (2004)
12 J.Q. Mao, T.J. Li, Y. Qiu, Y.C. Rui, and Z.B. Gu, J. Pharm. Pract., 25, 10–12 (2007)
13 Z.B. Gu, G.J. Yang, H.Y. Cong, Y.X. Xu, H.S. Chen, and W.D. Zhang, Chin. Tradit. Herb. Drugs, 33, 781–782 (2002)
DOI :
Qute : Gwarda, R. Ł. ,Tomczyszyn, A. ,Misicka, A. ,Dzido, T. H. ,Xue, X. D. ,Zhang, M. ,Xie, S. M. ,Yuan, L. M. ,Matta, M. K. ,Pilli, N. R. ,Rao, J. V. L. N. S. ,Abdelhay, M. H. ,Gazy, A. A. ,Shaalan, R. A. ,Ashour, H. K. ,Krasucka, D. ,Kowalski, C. J. ,Osypiuk, M. ,Opielak, G. ,Mahgoub, H. ,Hanafy, A. ,Bamane, F. H. ,Radwan, A. E. ,Wen, C. ,Zhang, Q. ,He, Y. ,Deng, M. ,Wang, X. ,Ma, J. ,Wang, Y. ,Wang, M. J. ,Li, J. ,Yao, S. C. ,Xue, J. ,Zou, W. B. ,Hu, C. ,Idris, A. M. ,Alnajjar, A. O. ,Vichapong, J. ,Burakham, R. ,Srivastava, P. ,Raut, H. N. ,Puntambekar, H. M. ,Desai, A. C. ,Wang, F. ,Liu, S. ,Luo, M. Y. ,Qin, Y. ,Lei, P. ,Liu, Y. H. ,Liang, Y. Z. ,Liu, W. ,Wei, M. L. ,Wu, C. Y. ,Zhu, H. Y. ,Feng, F. ,Xie, N. ,Xie, N. , Fingerprint of ethyl acetate extraction combined with qualitative and quantitative analysis on Patrinia scabra Bunge: Distinguish P. scabra Bunge from its confusable species. Acta Chromatographica Vol. 27, no. 1/2015
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