First detailed quantification of silymarin components in the leaves of Silybum marianum cultivated in egypt during different growth stages

Czasopismo : Acta Chromatographica
Tytuł artykułu : First detailed quantification of silymarin components in the leaves of Silybum marianum cultivated in egypt during different growth stages

Autorzy :
Bhushan, R.
Indian Institute of Technology Roorkee Department of Chemistry Roorkee 247667 India, rbushfcy@iitr.ernet.in,
Dixit, S.
Indian Institute of Technology Roorkee Department of Chemistry Roorkee 247667 India,
Mátyus, M.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Kocsis, G.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Boldis, O.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Karvaly, G.
Scientific Institute Military Hospital-State Health Centre P.O. Box 1 H-1553 Budapest Hungary,
Magyari, E.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Fűrész, J.
Scientific Institute Military Hospital-State Health Centre P.O. Box 1 H-1553 Budapest Hungary,
Gachályi, A.
Hungarian Defence Forces Dr György Radó Military Medical Center Budapest Hungary,
Mantovani, L.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil,
Sayago, C. T. M.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil,
Camargo, V. B.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil,
Silveira, V. F.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil,
Garcia, C. V.
Universidade Federal do Rio Grande do Sul Programa de Pós-graduação em Ciências Farmacêuticas Av. Ipiranga 2752 Lab 402 CEP 90610-000 Porto Alegre-RS Brazil,
Schapoval, E. E. S.
Universidade Federal do Rio Grande do Sul Programa de Pós-graduação em Ciências Farmacêuticas Av. Ipiranga 2752 Lab 402 CEP 90610-000 Porto Alegre-RS Brazil,
Mendez, A. S. L.
Universidade Federal do Pampa Laboratório de Pesquisa e Desenvolvimento em Controle de Qualidade de Medicamentos BR 472 km 585 Prédio Administrativo CEP 97500-970 Uruguaiana-RS Brazil, andreaslmendez@yahoo.com.br,
Cao, L.-W.
Jinan University Department of Chemistry Guangzhou 510632 People’s Republic of China, lwcao@126.com,
Li, C.
Jinan University Department of Chemistry Guangzhou 510632 People’s Republic of China,
Desai, D.
The Maharaja Sayajirao University of Baroda Quality Assurance Laboratory, Centre of Relevance and Excellence in Novel Drug Delivery System, Pharmacy Department, G. H. Patel Pharmacy Building, Donor’s Plaza Fatehgunj, Vadodara 390 002 Gujarat India,
Patel, G.
The Maharaja Sayajirao University of Baroda Quality Assurance Laboratory, Centre of Relevance and Excellence in Novel Drug Delivery System, Pharmacy Department, G. H. Patel Pharmacy Building, Donor’s Plaza Fatehgunj, Vadodara 390 002 Gujarat India,
Shukla, N.
The Maharaja Sayajirao University of Baroda Quality Assurance Laboratory, Centre of Relevance and Excellence in Novel Drug Delivery System, Pharmacy Department, G. H. Patel Pharmacy Building, Donor’s Plaza Fatehgunj, Vadodara 390 002 Gujarat India,
Rajput, S.
The Maharaja Sayajirao University of Baroda Quality Assurance Laboratory, Centre of Relevance and Excellence in Novel Drug Delivery System, Pharmacy Department, G. H. Patel Pharmacy Building, Donor’s Plaza Fatehgunj, Vadodara 390 002 Gujarat India, sjrajput@gmail.com,
Joshi, S. S.
National Institute of Pharmaceutical Education and Research (NIPER) Balanagar, Hyderabad 500037 India,
Nahire, R. R.
National Institute of Pharmaceutical Education and Research (NIPER) Balanagar, Hyderabad 500037 India,
Shastri, N. R.
National Institute of Pharmaceutical Education and Research (NIPER) Balanagar, Hyderabad 500037 India, svcphod@yahoo.co.in,
Surendranath, K. V.
ICICI Knowledge Park United States Pharmacopeia India Private Limited, Reference Standard Laboratory Shameerpet, Hyderabad 500078 India,
Satish, J.
ICICI Knowledge Park United States Pharmacopeia India Private Limited, Reference Standard Laboratory Shameerpet, Hyderabad 500078 India,
Kobyłka, M.
Medical University of Lublin Department of Medicinal Chemistry Jaczewskiego 4 20-090 Lublin Poland,
Komsta, Ł.
Medical University of Lublin Department of Medicinal Chemistry Jaczewskiego 4 20-090 Lublin Poland, lukasz.komsta@umlub.pl,
Adegoke, O. A.
University of Ibadan Department of Pharmaceutical Chemistry, Faculty of Pharmacy Ibadan Nigeria, ao.adegoke@mail.ui.edu.ng,
Xiang, L. L.
Chinese Academy of Sciences Laboratory of Pharmaceutical Analysis, Shanghai Institute of Materia Medica 555 Zuchongzhi Road Shanghai 201203 PR China,
Idowu, O. S.
University of Ibadan Department of Pharmaceutical Chemistry, Faculty of Pharmacy Ibadan Nigeria,
Chen, D.-Y.
Chinese Academy of Sciences Laboratory of Pharmaceutical Analysis, Shanghai Institute of Materia Medica 555 Zuchongzhi Road Shanghai 201203 PR China, dychen@mail.shcnc.ac.cn,
Omar, A. A.
Alexandria University Department of Pharmacognosy, Faculty of Pharmacy Alexandria 21521 Egypt,
Hadad, G. M.
Suez Canal University Department of Analytical Chemistry, Faculty of Pharmacy Ismailia 41522 Egypt,
Badr, J. M.
King Abdulaziz University Department of Natural Products and Alternative Medicine, Faculty of Pharmacy Jeddah 21589 Kingdom of Saudi Arabia, jihanbadr2010@hotmail.com,
Abstrakty : A high performance liquid chromatographic method was developed for the quantitative determination of the active components of silymarin in the leaves of Silybum marianum during different growth stages. In this study, taxifolin and six main active constituents in silymarin, including silydianin, silychristin, diastereomers of silybin (silybin A and B) and diastereomers of isosilybin (isosilybin A and B) were completely separated on a 5-μm ODS column (Luna, Phenomenex, USA) with a mobile phase consisting of methanol and 5 mM NaH2PO4 (pH 3.5 adjusted with phosphoric acid) in a ratio of 45:55 v/v. Quantitation was performed with UV detection at 280 nm, based on peak area. The concentration of each component, as well as the total silymarin concentration was determined and compared with those of the seeds, aiming at optimizing the utilization of the cultivated plant. The developed method was validated with respect to linearity, range, specificity, accuracy, precision, and robustness. The leaves were found to contain such concentrations of silymarin components that the yield is better per field area than that from the seeds. Moreover, the extraction of these components from leaves is nonexpensive and simpler than the extraction from seeds.

Słowa kluczowe : Silybum marianum, flavolignans, leaves, growth stages, HPLC,
Wydawnictwo : University of Silesia in Katowice
Rocznik : 2012
Numer : Vol. 24, no. 3
Strony : 463 – 473
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DOI :
Cytuj : Bhushan, R. ,Dixit, S. ,Mátyus, M. ,Kocsis, G. ,Boldis, O. ,Karvaly, G. ,Magyari, E. ,Fűrész, J. ,Gachályi, A. ,Mantovani, L. ,Sayago, C. T. M. ,Camargo, V. B. ,Silveira, V. F. ,Garcia, C. V. ,Schapoval, E. E. S. ,Mendez, A. S. L. ,Cao, L.-W. ,Li, C. ,Desai, D. ,Patel, G. ,Shukla, N. ,Rajput, S. ,Joshi, S. S. ,Nahire, R. R. ,Shastri, N. R. ,Surendranath, K. V. ,Satish, J. ,Kobyłka, M. ,Komsta, Ł. ,Adegoke, O. A. ,Xiang, L. L. ,Idowu, O. S. ,Chen, D.-Y. ,Omar, A. A. ,Hadad, G. M. ,Badr, J. M. , First detailed quantification of silymarin components in the leaves of Silybum marianum cultivated in egypt during different growth stages. Acta Chromatographica Vol. 24, no. 3/2012
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