Journal : Acta Chromatographica
Article : HPLC analysis and detection of l-deprenyl

Authors :
Kuc, J.
Cracow University of Technology Department of Chemical Engineering and Technology, Department of Analytical Chemistry Warszawska 24 31-155 Cracow Poland, jkuc@chemia.pk.edu.pl,
Grochowalski, A.
Cracow University of Technology Department of Chemical Engineering and Technology, Department of Analytical Chemistry Warszawska 24 31-155 Cracow Poland,
Mach, S.
Institute of Public Health Ostrava Centre of Hygienic Laboratories Dobrá 240 739 51 Frýdek-Místek Czech Republic,
Placha, D.
VŠB-Technical University of Ostrava Centrum Nanotechnology 17 Listopadu 15/2172 708 33 Ostrava Czech Republic,
Furusawa, N.
Osaka City University Graduate School of Human Life Science Osaka 558-8585 Japan,
Stoilova, N.
Bulgarian Food Safety Agency Central Laboratory of Veterinary Control and Ecology (CLVCE) 5 “Iskarsko shousse” str., 1528 Sofia Bulgaria, nadqstoilova@abv.bg,
Surleva, A.
University of Chemical Technology and Metallurgy Analytical Chemistry Department 8 “St. Kl. Ohridski” blvd., 1756 Sofia Bulgaria,
Stoev, G.
Bulgarian Food Safety Agency Central Laboratory of Veterinary Control and Ecology (CLVCE) 5 “Iskarsko shousse” str., 1528 Sofia Bulgaria,
Popovic, N.
Lafayette College Department of Chemistry Easton PA USA,
Sherma, J.
Lafayette College Department of Chemistry Easton PA USA, shermaj@lafayette.edu,
Dixit, S.
Yeungnam University Department of Chemistry Gyeongsan 712-749 South Korea, drdixit.shuchi@gmail.com,
Dubey, R.
riturajdubey0@gmail.com,
Bhushan, R.
Indian Institute of Technology Roorkee Department of Chemistry Roorkee 247667 India, rbushfcy@iitr.ernet.in,
Fouad, M. A.
Cairo University Pharmaceutical Chemistry Department, Faculty of Pharmacy Kasr El-Aini St. Cairo 11562 Egypt, merro911@yahoo.com,
Elkady, E. F.
Cairo University Pharmaceutical Chemistry Department, Faculty of Pharmacy Kasr El-Aini St. Cairo 11562 Egypt,
Csomor, V.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Laufer, R.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Pöstényi, Z.
Semmelweis University Department of Pharmacology and Pharmacotherapy Budapest Hungary,
Kalász, H.
Semmelweis University Department of Pharmacology and Pharmacotherapy Budapest Hungary, huba.kalasz@gmail.com,
Adeghate, E.
United Arab Emirates University Department of Anatomy Al Ain UAE,
Nurulain, S. M.
United Arab Emirates University Department of Pharmacology and Therapeutics Al Ain UAE,
Tekes, K.
Semmelweis University Department of Pharmacodynamics Budapest Hungary,
Adem, A.
United Arab Emirates University Department of Pharmacology and Therapeutics Al Ain UAE,
Abstract : l-Deprenyl and its major metabolites were subjected to reversed-phase high-performance liquid chromatography (HPLC). The separation was monitored using both ultraviolet (UV) and electrochemical detectors. Ultraviolet absorbance detected l-deprenyl, l-nordeprenyl, l-methamphetamine, and l-amphetamine. Amperometric detection was specific and sensitive to the parent compound (l-deprenyl, a tertiary amine) only. Peaks of the major L-deprenyl metabolites did not give any comparable signal using amperometric monitoring.

Keywords : l-deprenyl, HPLC, amperometric detection, UV detection, metabolites,
Publishing house : University of Silesia in Katowice
Publication date : 2014
Number : Vol. 26, no. 4
Page : 649 – 656

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DOI :
Qute : Kuc, J. ,Grochowalski, A. ,Mach, S. ,Placha, D. ,Furusawa, N. ,Stoilova, N. ,Surleva, A. ,Stoev, G. ,Popovic, N. ,Sherma, J. ,Dixit, S. ,Dubey, R. ,Bhushan, R. ,Fouad, M. A. ,Elkady, E. F. ,Csomor, V. ,Laufer, R. ,Pöstényi, Z. ,Kalász, H. ,Adeghate, E. ,Nurulain, S. M. ,Tekes, K. ,Adem, A. ,Adem, A. , HPLC analysis and detection of l-deprenyl. Acta Chromatographica Vol. 26, no. 4/2014
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