Journal : Acta Chromatographica
Article : HPLC analysis of Phyllanthus amarus samples stored in stability chambers under different conditions and study of the effect on quantification of the phytomarkers phyllanthin and hypophyllanthin

Authors :
Gwarda, R. Ł.
Medical University of Lublin Department of Physical Chemistry, Chair of Chemistry 4a Chodźki Street 20-093 Lublin Poland, radoslaw.gwarda@umlub.pl,,
Tomczyszyn, A.
University of Warsaw Faculty of Chemistry 1 Pasteura Street 02-093 Warsaw Poland,
Misicka, A.
University of Warsaw Faculty of Chemistry 1 Pasteura Street 02-093 Warsaw Poland,
Dzido, T. H.
Medical University of Lublin Department of Physical Chemistry, Chair of Chemistry 4a Chodźki Street 20-093 Lublin Poland,
Xue, X. D.
Yunnan Normal University Department of Chemistry Kunming 650092 P.R. China,
Zhang, M.
East China Normal University Department of Chemistry Shanghai 200241 P.R. China,
Xie, S. M.
Yunnan Normal University Department of Chemistry Kunming 650092 P.R. China,
Yuan, L. M.
Yunnan Normal University Department of Chemistry Kunming 650092 P.R. China, yuan_limingpd@yahoo.com.cn,
Matta, M. K.
Jawaharlal Nehru Technological University University College of Pharmaceutical Sciences Hyderabad 500 085 India,
Pilli, N. R.
Jawaharlal Nehru Technological University University College of Pharmaceutical Sciences Hyderabad 500 085 India,
Rao, J. V. L. N. S.
Yalamarty College of Pharmaceutical Sciences Visakhapatnam 530 052 India, jvlnsrao@rediffmail.com,
Abdelhay, M. H.
University of Alexandria Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Elmessalah, Alexandria 21521 Egypt,
Gazy, A. A.
University of Alexandria Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Elmessalah, Alexandria 21521 Egypt,
Shaalan, R. A.
rasha_shaalan@yahoo.com,
Ashour, H. K.
University of Alexandria Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Elmessalah, Alexandria 21521 Egypt,
Krasucka, D.
National Veterinary Research Institute — National Research Institute Department of Veterinary Pharmacy Al. Partyzantów 57 24-100 Pulawy Poland,
Kowalski, C. J.
University of Life Sciences in Lublin Department of Pharmacology, Faculty of Veterinary Medicine Akademicka 12 20-033 Lublin Poland,
Osypiuk, M.
University of Life Sciences in Lublin Department of Pharmacology, Faculty of Veterinary Medicine Akademicka 12 20-033 Lublin Poland, monika.osypiuk@up.lublin.pl,
Opielak, G.
Medical University School of Lublin Human Anatomy Department Jaczewskiego 4 20-090 Lublin Poland,
Mahgoub, H.
King Abdulaziz University Department of Pharmaceutical Chemistry, Faculty of Pharmacy Jeddah Saudi Arabia, hodamahgoub@yahoo.com,
Hanafy, A.
King Abdulaziz University Department of Pharmacology and Toxicology, Faculty of Pharmacy Jeddah Saudi Arabia,
Bamane, F. H.
King Abdulaziz University Department of Biochemistry, Faculty of Medicine Jeddah Saudi Arabia,
Radwan, A. E.
University of Alexandria Department of Biotechnology, Institute of Graduate Studies and Research Alexandria Egypt,
Wen, C.
Wenzhou Medical College Laboratory Animal Centre Wenzhou 325035 China,
Zhang, Q.
Shanghai Institute of Pharmaceutical Industry Shanghai 200437 China,
He, Y.
Wenzhou Medical College Laboratory Animal Centre Wenzhou 325035 China,
Deng, M.
Wenzhou Medical College Laboratory Animal Centre Wenzhou 325035 China,
Wang, X.
Wenzhou Medical College Laboratory Animal Centre Wenzhou 325035 China,
Ma, J.
Wenzhou Medical College Function Experiment Teaching Center Wenzhou 325035 China, jianshema@gmail.com,
Wang, Y.
National Institutes for Food and Drug control (NIFDC) Beijing 100050 P.R. China,
Wang, M. J.
National Institutes for Food and Drug control (NIFDC) Beijing 100050 P.R. China,
Li, J.
National Institutes for Food and Drug control (NIFDC) Beijing 100050 P.R. China,
Yao, S. C.
National Institutes for Food and Drug control (NIFDC) Beijing 100050 P.R. China,
Xue, J.
National Institutes for Food and Drug control (NIFDC) Beijing 100050 P.R. China,
Zou, W. B.
National Institutes for Food and Drug control (NIFDC) Beijing 100050 P.R. China,
Hu, C.
National Institutes for Food and Drug control (NIFDC) Beijing 100050 P.R. China, hucq@nicpbp.org.cn,
Idris, A. M.
King Khalid University Department of Chemistry, College of Science Abha Saudi Arabia, abubakridris@hotmail.com,
Alnajjar, A. O.
King Faisal University Department of Chemistry, College of Science Hofuf Saudi Arabia,
Vichapong, J.
Khon Kaen University Materials Chemistry Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science Khon Kaen 40002 Thailand,
Burakham, R.
Khon Kaen University Materials Chemistry Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science Khon Kaen 40002 Thailand, rodjbu@kku.ac.th,
Srivastava, P.
Agharkar Research Institute Chemistry Group, Animal Science Division G. G. Agarkar Road Pune 411004 India, psri94@yahoo.com,
Raut, H. N.
Agharkar Research Institute Chemistry Group, Animal Science Division G. G. Agarkar Road Pune 411004 India,
Puntambekar, H. M.
Agharkar Research Institute Chemistry Group, Animal Science Division G. G. Agarkar Road Pune 411004 India,
Desai, A. C.
Indian Drug Research Association and Laboratory Drug Analysis 561/B, Shivajinagar Pune 411005 India,
Abstract : Storage of medicinal plants may cause deterioration of the active principles with time, thus, reducing the efficacy of plants. Therefore, quantification of the active principle is essential before using the crude drug. Phyllanthus amarus (PA) contains lignans, namely, phyllanthin and hypophyllanthin, which shows anti-hepatotoxic activity. In this paper, we highlight the effect of storage conditions on the quantification of bioactive markers by high-performance liquid chromatography (HPLC) analysis in the crude plant material of PA. HPLC analysis of crude PA samples stored for certain period at long-term study (LS, 30 °C and 65% RH), accelerated study (AS, 40°C and 75% RH), and real-time study (RT) conditions was carried out using the LiChroCART Purospher® STAR RP-18 endcapped (250 × 4.6 mm, 5 μm) column along with a Purospher STAR RP 18e (4.0 × 4.0 mm, 5 μm) guard column using methanol:water (70:30) at a flow rate of 0.7 mL min−1 with ultraviolet (UV) detection at 220 nm. The HPLC study indicated that PA samples kept under LS condition are rich in lignan contents as compared to the samples stored under AS and RT study conditions. Therefore, PA should be used fresh to get maximum concentration of active lignans or it should be stored under LS conditions up to 6 months.

Keywords : Phyllanthus amarus, quantitative estimation, phyllanthin, hypophyllanthin, stability studies, HPLC analysis,
Publishing house : University of Silesia in Katowice
Publication date : 2015
Number : Vol. 27, no. 1
Page : 147 – 156

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DOI :
Qute : Gwarda, R. Ł. ,Tomczyszyn, A. ,Misicka, A. ,Dzido, T. H. ,Xue, X. D. ,Zhang, M. ,Xie, S. M. ,Yuan, L. M. ,Matta, M. K. ,Pilli, N. R. ,Rao, J. V. L. N. S. ,Abdelhay, M. H. ,Gazy, A. A. ,Shaalan, R. A. ,Ashour, H. K. ,Krasucka, D. ,Kowalski, C. J. ,Osypiuk, M. ,Opielak, G. ,Mahgoub, H. ,Hanafy, A. ,Bamane, F. H. ,Radwan, A. E. ,Wen, C. ,Zhang, Q. ,He, Y. ,Deng, M. ,Wang, X. ,Ma, J. ,Wang, Y. ,Wang, M. J. ,Li, J. ,Yao, S. C. ,Xue, J. ,Zou, W. B. ,Hu, C. ,Idris, A. M. ,Alnajjar, A. O. ,Vichapong, J. ,Burakham, R. ,Srivastava, P. ,Raut, H. N. ,Puntambekar, H. M. ,Desai, A. C. ,Desai, A. C. , HPLC analysis of Phyllanthus amarus samples stored in stability chambers under different conditions and study of the effect on quantification of the phytomarkers phyllanthin and hypophyllanthin. Acta Chromatographica Vol. 27, no. 1/2015
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