Journal : Acta Chromatographica
Article : HPLC analysis of microsomal metabolism of K-48

Authors :
Sherma, J.
Department of Chemistry, Lafayette College, Easton, PA 18042, USA,
Vrchotová, N.
Institute of Systems Biology and Ecology AS CR, Branišovská 31, 370 05 České Budějovice, Czech Republic,
Sivakumar, T.
Department of Pharmacy, Faculty of Engineering and Technology, Annamalai University, Annamalainagar, Tamil Nadu-608 002, India,
Petruczynik, A.
Department of Inorganic Chemistry, Medical University, Staszica 6, 20-081 Lublin, Poland,
Benkő, B.
PhD School of Semmelweis University, 1085 Budapest, Üllői út 26, Hungary, Division of Pharmacology and Drug Safety, Richter Gedeon Rt., Gyömrői út 21, 1475 Budapest, P.O. Box 27, Hungary, Department of Chemistry and Biochemistry, University of Pécs, Péc,
Abstract : K-27 and K-48, bisquaternary pyridinium aldoximes differing only in the number of methyl units in the alkyl bridges between the two quaternary nitrogen atoms, are promising candidates for use as potent and effective antidotes to organophosphate intoxication. K-27 and K-48 were treated with rat hepatic microsomal preparations from healthy (control) and diabetic animals. Microsomal metabolism was measured at different times up to 45 min. This treatment resulted in a decrease of approximately 30% in the concentration of K-48 whereas that of K-27 was unchanged. There was no significant difference between the effects of microsomal preparations from control and diabetic rat liver. K-27 and K-48 in a variety of media were analysed by reversed-phase HPLC with both ultraviolet and electrochemical detection. The method was validated by the usual procedures.

Keywords :
Publishing house : University of Silesia in Katowice
Publication date : 2007
Number : No. 19
Page : 61 – 72

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DOI :
Qute : Sherma, J. ,Vrchotová, N. ,Sivakumar, T. ,Petruczynik, A. ,Benkő, B. ,Benkő, B. , HPLC analysis of microsomal metabolism of K-48. Acta Chromatographica No. 19/2007
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