Journal : Acta Chromatographica
Article : HPLC behavior of sulfonamides on molecularly imprinted polymeric stationary phases

Authors :
Bartnik, M.
Department of Pharmacognosy with Medicinal Plant Laboratory, Medical University, 1, Chodźki Str., 20-093 Lublin, Poland,
Kowalski, R.
Subdepartment of Food Quality Assessment and Central Apparatus Laboratory, Agricultural University, 20-950 Lublin, Akademicka 13, Poland,
Polak, B.
Department of Physical Chemistry, Faculty of Pharmacy, Medical University, Lublin, Poland,
Turło, J.
Department of Drug Technology, Medical University of Warsaw, 1, Banacha Street, 02-097 Warsaw, Poland,
Szyrwińska, K.
Department of Inorganic and Analytical Chemistry, Faculty of Pharmacy, Poznań University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, Poland,
Czauderna, M.
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland,
Bączek, T.
Medical University of Gdańsk, Department of Biopharmaceutics and Pharmacodynamics, Gdańsk, Poland,
Chilmonczyk, Z.
National Medicines Institute, 30/34, Chełmska Str., 00-725 Warsaw, Poland,
Hung, Ch. Y.
Department of Biotechnology, National Formosa University, Huwei, Yunlin, 632, Taiwan,
Abstract : The purpose of this study was to develop an HPLC procedure for determination of sulfamethazine (SMZ) and sulfachloropyridazine (SCP) on a molecularly imprinted polymeric (MIP) stationary phase. The MIPs were prepared by noncovalent polymerization. Sulfamethazine (SMZ), methacrylic acid (MAA), and ethylene glycoldimethacrylate (EGDMA) were used as template, functional monomer, and cross-linker, respectively, in the presence of chloroform as solvent. The MIPs obtained were packed into a stainless steel column (150 mm × 4.6 mm i.d.) and used as the stationary phase for HPLC. The mobile phase was 23 mM NaH2PO4 buffer solution–acetonitrile, 2:1 (v/v), at a flow-rate of 1 mL min-1. The sample volume injected was 20 µL and detection was by UV absorbance at 272 nm. The retention times of SMZ and SCP under these conditions were approximately 7.015 ± 0.182 min and 11.282 ± 0.925 min. The total run time for each sample was 12 min. The selectivity of the MIPs was evaluated by analysis of different substances with molecular structures similar to that of SMZ. Separation factors (α) were used to compare chromatographic data from the stationary phases. The values of α obtained, in the range 1.57–1.72, showed that use of the MIPs enabled recognition of subtle structural differences from the template molecule. The limits of detection (LOD) of SMZ and SCP were 0.035 and 0.041 µg L-1, respectively, and the respective limits of quantitation (LOQ) were 0.117 and 0.137 µg L-1. The results showed that the SMZ-selective polymer was suitable for separating both SMZ and SCP.

Keywords :
Publishing house : University of Silesia in Katowice
Publication date : 2007
Number : No. 18
Page : 106 – 115

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Qute : Bartnik, M. ,Kowalski, R. ,Polak, B. ,Turło, J. ,Szyrwińska, K. ,Czauderna, M. ,Bączek, T. ,Chilmonczyk, Z. ,Hung, Ch. Y. ,Hung, Ch. Y. , HPLC behavior of sulfonamides on molecularly imprinted polymeric stationary phases. Acta Chromatographica No. 18/2007