Journal : Acta Chromatographica
Article : High-performance thin-layer chromatographic analysis of psoralen in marketed formulations and manufactured solid lipid nanoparticles (SLNs): Validation of the method

Authors :
Xu, L. N.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Gai, F. Y.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Mu, G. F.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Gao, Y.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Liu, C. J.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Luan, F.
Yantai University Department of Applied Chemistry Yantai 264005 P.R. China,
Mori, M.
Gunma University Department of Chemistry and Chemical Biology, Graduate School of Engineering 1-5-1 Tenjin-cho Kiryu 376-8515 Japan,
Ikeda, T.
Gunma University Department of Chemistry and Chemical Biology, Graduate School of Engineering 1-5-1 Tenjin-cho Kiryu 376-8515 Japan,
Itabashi, H.
Gunma University Department of Chemistry and Chemical Biology, Graduate School of Engineering 1-5-1 Tenjin-cho Kiryu 376-8515 Japan,
Soomro, R.
University of Sindh National Center of Excellence in Analytical Chemistry Jamshoro Pakistan,
Memon, S. Q.
University of Sindh Institute of Advance Research in Chemical Sciences Jamshoro Pakistan,
Ahmed, M. J.
University of Chittagong Laboratory of Analytical Chemistry, Department of Chemistry Chittagong 4331 Bangladesh,
Memon, N.
University of Sindh National Center of Excellence in Analytical Chemistry Jamshoro Pakistan, najmamemon@gmail.com,
Mallah, A.
University of Sindh M.A. Kazi Institute of Chemistry Jamshoro Pakistan,
Ravi, P. R.
BITS-Pilani Hyderabad Campus Pharmacy Department Ranga Reddy (Dist.) Jawaharnagar Andhra Pradesh India, rahulvats1983@gmail.com,
Aditya, N.
BITS-Pilani Hyderabad Campus Pharmacy Department Ranga Reddy (Dist.) Jawaharnagar Andhra Pradesh India, adityamurthy1212@gmail.com,
Vats, R.
BITS-Pilani Hyderabad Campus Pharmacy Department Ranga Reddy (Dist.) Jawaharnagar Andhra Pradesh India, rpunnarao@gmail.com,
Navaneethan, G.
Sona College of Technology (Anna University) Department of Sciences Salem 636 005 India,
Karunakaran, K.
Sona College of Technology (Anna University) Department of Sciences Salem 636 005 India, drkk@sonatech.ac.in,
Elango, K. P.
Gandhigram Rural Institute (Deemed University) Department of Chemistry Gandhigram 624 302 India,
Prichodko, A.
Vilnius University Department of Analytical and Environmental Chemistry Naugarduko 24 LT-03225 Vilnius Lithuania,
Janenaite, E.
Vilnius University Department of Analytical and Environmental Chemistry Naugarduko 24 LT-03225 Vilnius Lithuania,
Smitiene, V.
Vilnius University Department of Analytical and Environmental Chemistry Naugarduko 24 LT-03225 Vilnius Lithuania,
Vickackaite, V.
Vilnius University Department of Analytical and Environmental Chemistry Naugarduko 24 LT-03225 Vilnius Lithuania, vida.vickackaite@chf.vu.lt,
Akhtar, N.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India,
Faiyazuddin, M.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India, md.faiyazuddin@yahoo.co.in,
Mustafa, G.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India,
Sultana, Y.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India,
Baboota, S.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India,
Ali, J.
Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India,
Abstract : A quantitative method using precoated silica gel-60 Lichrosphere high-performance thin-layer chromatography (HPTLC) plates, automated band wise sample application, and n-hexane:acetone:formic acid (2:1:0.025 υ/υ/υ) as mobile phase, has been developed and validated for the analysis of psoralen in marketed formulations and novel solid lipid nanoparticles (SLNs). Densitometric analysis was performed at 250 nm in absorbance mode. Compact bands of psoralen were obtained at Rf 0.32 ± 0.02. The method was validated for linearity, precision, robustness, sensitivity, specificity, and recovery. Linearity (r2 = 0.995), limit of detection (8.0 ng band-1), limit of quantification (18.1 ng band)-1, recovery (98.06–99.64%), and precision (≤0.74) were satisfactory. Statistical analysis established that the developed method for quantification of psoralen in marketed formulation and from solid lipid nanoparticles is reproducible and selective.

Keywords : psoralen, Babchi oil, high-performance thin-layer chromatography, validation, solid lipid nanoparticles,
Publishing house : University of Silesia in Katowice
Publication date : 2012
Number : Vol. 24, no. 4
Page : 603 – 613

Bibliography
: 1 T.F. Anderson and J.J. Voorhees, Annual Rev. Pharmacol. Toxicol., 20, 235 (1980)
2 E.M. Farber and M.L. Nall, Dermatologica, 148, 1, (1974)
3 L. Chang, Psoriasis Phototherapy: UVA beats UVB, WebMD Medical News Daniel DeNoon. 18 (2006)
4 N.J. Lowe, V. Chizhevsky, and H. Gabriel, Clin. Dermatol., 15, 745 (1997)
5 J.E. Hearst, J. Invest. Dermatol., 77, 39 (1981)
6 L. Musajo, F. Bordin, and R. Bevilacqua, Photochem. Photobiol., 6, 927 (1967)
7 V.C. Leite, R.F. Santos, and L.C. Chen, J. Photochem. Photobiol. B: Biology., 76, 49 (2004)
8 C.A.L. Cardoso, N.K. Honda, and A. Barison, J. Pharm. Biomed. Anal. 27, 217 (2002)
9 N.T. Dong, K. Bae, Y.H. Kim, G.S. Hwang, O.S. Heo, S.E. Kim, and J.S. Kang, Arch. Pharm. Res., 26, 516 (2003)
10 A.E. Pires, N.K. Honda, and C.A.L. Cardoso, J. Pharm. Biomed. Anal., 36, 415 (2004)
11 Y. Yan, H.S. Tan, and C. Guinchard, Ann. Pharm. Fr. 52, 160 (1994)
12 Md. Faiyazuddin, S. Ahmad, Z. Iqbal, S. Talegaonkar, F.J. Ahmad, A. Bhatnagar, and R.K. Khar, Anal. Sci., 26, 1 (2010)
13 Md. Faiyazuddin, N. Ahmad, S. Baboota, J. Ali, S. Ahmad, and J. Akhter, J. Planar Chromatogr. JPC., 23, 3 (2010)
14 M. Faiyazuddin, N. Akhtar, J. Akhter, F. Shakeel, S. Shafiq, and G. Mustafa, Pharmazie, 65, 1 (2010)
DOI :
Qute : Xu, L. N. ,Gai, F. Y. ,Mu, G. F. ,Gao, Y. ,Liu, C. J. ,Luan, F. ,Mori, M. ,Ikeda, T. ,Itabashi, H. ,Soomro, R. ,Memon, S. Q. ,Ahmed, M. J. ,Memon, N. ,Mallah, A. ,Ravi, P. R. ,Aditya, N. ,Vats, R. ,Navaneethan, G. ,Karunakaran, K. ,Elango, K. P. ,Prichodko, A. ,Janenaite, E. ,Smitiene, V. ,Vickackaite, V. ,Akhtar, N. ,Faiyazuddin, M. ,Mustafa, G. ,Sultana, Y. ,Baboota, S. ,Ali, J. ,Ali, J. , High-performance thin-layer chromatographic analysis of psoralen in marketed formulations and manufactured solid lipid nanoparticles (SLNs): Validation of the method. Acta Chromatographica Vol. 24, no. 4/2012
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